PCR detection reagent for detecting liver cancer core signal molecules and application thereof

Abstract

The invention provides a PCR detection reagent for detecting liver cancer core signal molecules and an application thereof. The PCR detection reagent comprises PCR reaction primers for detecting ADAM17 genes, AKT1 genes, ANGPT2 genes, BAX genes, BCL2 genes, BCL2L1 genes and other related genes, reference genes and the like. According to the invention, liver cancer core signal molecules are concentrated on a flat plate; by performing one-time real-time fluorescent quantitative PCR reaction, the survival state of cells is reflected, and compared with human liver cancer Huh7 and normal liver cells, possible ways of liver cancer core signal molecules in the human liver cancer and the normal liver cells are discussed, and most direct evidences are provided for researching regulation and controlof key proteins; according to the invention, the liver cancer core signal molecule is rapidly and accurately found from the transcriptional level, and a powerful tool is provided for mechanism discussion of new targeting drugs, development of targeting liver cancer inhibitors and the like.

Description

Technical field [0001] The invention belongs to the field of molecular biology, and particularly relates to a PCR detection reagent for detecting core signal molecules of liver cancer and its application. Background technique [0002] Hepatocellular carcinoma (HCC) is the fifth most common tumor in the world and the third in tumor-related deaths, especially in Asia and Africa, which are severely affected by hepatitis viruses. Although there are many treatment methods for liver cancer, such as surgical treatment, transhepatic artery chemoembolization, local ablation and radiotherapy, the overall curative effect is poor. Most of the liver cancer patients in my country occur on the basis of liver cirrhosis, and most patients have advanced liver cancer at the time of treatment. Less than 20% can be surgically removed, and there are limited patients suitable for local ablation. In recent years, with the in-depth research and development of molecular biology, it has been found that ma...

AI Technical Summary

Problems solved by technology

Although there are many treatment methods for liver cancer, such as surgery, transarterial chemoembolization, local ablation, and radiotherapy, the overall curative effect is poor.

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