PCR detection reagent for detecting liver cancer core signal molecules and application thereof
A core signal and detection reagent technology, applied in the field of molecular biology, can solve the problem of poor overall curative effect
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[0293] The present invention is exemplified by human liver cancer Huh7 and normal liver cell liver cell, but the present invention is not limited to these two types of cells. The detection reagent of the present invention can be applied to liver cancer cell lines including SMMC7721, HCCLM3, etc., and can also detect clinical Liver cancer tissues and adjacent tissues, etc.
[0294] In the present invention, two cell lines are cultured: human liver cancer Huh7 and normal liver cell liver cell, and then the core angiogenic molecule changes in the two cell lines are detected by a liver cancer core molecule detection reagent.
[0295] The human liver cancer Huh7 and normal hepatocyte liver cells used in the present invention were purchased from ATCC, Shanghai Cell Bank, DMEM medium, and fetal bovine serum were purchased from Gibco, USA, RNAiso for extracting total RNA, reverse transcription kit, 2× Premix Ex Taq kit was purchased from TaKaRa.
[0296] Using the PCR detection reagent of ...
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