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PCR detection reagent for detecting liver cancer core signal molecules and application thereof

A core signal and detection reagent technology, applied in the field of molecular biology, can solve the problem of poor overall curative effect

Pending Publication Date: 2020-10-30
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many treatment methods for liver cancer, such as surgery, transarterial chemoembolization, local ablation, and radiotherapy, the overall curative effect is poor.

Method used

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  • PCR detection reagent for detecting liver cancer core signal molecules and application thereof
  • PCR detection reagent for detecting liver cancer core signal molecules and application thereof
  • PCR detection reagent for detecting liver cancer core signal molecules and application thereof

Examples

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Embodiment 1

[0293] The present invention is exemplified by human liver cancer Huh7 and normal liver cell liver cell, but the present invention is not limited to these two types of cells. The detection reagent of the present invention can be applied to liver cancer cell lines including SMMC7721, HCCLM3, etc., and can also detect clinical Liver cancer tissues and adjacent tissues, etc.

[0294] In the present invention, two cell lines are cultured: human liver cancer Huh7 and normal liver cell liver cell, and then the core angiogenic molecule changes in the two cell lines are detected by a liver cancer core molecule detection reagent.

[0295] The human liver cancer Huh7 and normal hepatocyte liver cells used in the present invention were purchased from ATCC, Shanghai Cell Bank, DMEM medium, and fetal bovine serum were purchased from Gibco, USA, RNAiso for extracting total RNA, reverse transcription kit, 2× Premix Ex Taq kit was purchased from TaKaRa.

[0296] Using the PCR detection reagent of ...

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PUM

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Abstract

The invention provides a PCR detection reagent for detecting liver cancer core signal molecules and an application thereof. The PCR detection reagent comprises PCR reaction primers for detecting ADAM17 genes, AKT1 genes, ANGPT2 genes, BAX genes, BCL2 genes, BCL2L1 genes and other related genes, reference genes and the like. According to the invention, liver cancer core signal molecules are concentrated on a flat plate; by performing one-time real-time fluorescent quantitative PCR reaction, the survival state of cells is reflected, and compared with human liver cancer Huh7 and normal liver cells, possible ways of liver cancer core signal molecules in the human liver cancer and the normal liver cells are discussed, and most direct evidences are provided for researching regulation and controlof key proteins; according to the invention, the liver cancer core signal molecule is rapidly and accurately found from the transcriptional level, and a powerful tool is provided for mechanism discussion of new targeting drugs, development of targeting liver cancer inhibitors and the like.

Description

Technical field [0001] The invention belongs to the field of molecular biology, and particularly relates to a PCR detection reagent for detecting core signal molecules of liver cancer and its application. Background technique [0002] Hepatocellular carcinoma (HCC) is the fifth most common tumor in the world and the third in tumor-related deaths, especially in Asia and Africa, which are severely affected by hepatitis viruses. Although there are many treatment methods for liver cancer, such as surgical treatment, transhepatic artery chemoembolization, local ablation and radiotherapy, the overall curative effect is poor. Most of the liver cancer patients in my country occur on the basis of liver cirrhosis, and most patients have advanced liver cancer at the time of treatment. Less than 20% can be surgically removed, and there are limited patients suitable for local ablation. In recent years, with the in-depth research and development of molecular biology, it has been found that ma...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/686C12N15/11
CPCC12Q1/6886C12Q1/686C12Q2600/158C12Q2561/113C12Q2563/107C12Q2545/114
Inventor 刘世海贺桂芳刘畅畅蔡铎潘华政
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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