African swine fever virus droplet type digital PCR detection method and application thereof

Abstract

The invention discloses an African swine fever virus droplet type digital PCR detection method and application thereof. The African swine fever virus droplet type digital PCR detection method comprises the following steps: (1) designing and synthesizing primers, probes and recombinant plasmids; and (2) adopting a droplet digital PCR reaction to obtain a qualitative or quantitative result. The African swine fever virus droplet type digital PCR detection method is applied to detection of the African swine fever virus. Three pairs of primers and probes are optimized after design according to twoconserved sequences of African swine fever virus nucleic acid, the African swine fever virus ddPCR detection method is established, and the African swine fever virus ddPCR detection method has the advantages of strong specificity, higher sensitivity, higher accuracy, stronger anti-interference ability and better repeatability, and can effectively avoid the occurrence of false negative and false positive results; understanding and control of the African swine fever epidemic situation are facilitated, and strict control of pork dead of African swine fever and marketing of pork products in the market is also facilitated.

Description

technical field [0001] The invention relates to an African swine fever virus detection method and its application, in particular to a African swine fever virus droplet digital PCR detection method and its application. Background technique [0002] African swine fever (African swine fever, ASF) is an acute, hemorrhagic infectious disease of pigs caused by African swine fever virus (ASFV). The World Organization for Animal Health (Office International des Epizooties, OIE) listed it as a legally reported infectious disease, and my country listed it as a first-class infectious disease. The "National Medium and Long-term Animal Disease Prevention and Control Plan (2012-2020)" issued by the General Office of the State Council Among them, African swine fever is also one of the 13 foreign animal diseases that should be guarded against. African swine fever broke out in Georgia in April 2007. In November of the same year, the first case of African swine fever occurred in Russia, which ...

AI Technical Summary

Problems solved by technology

The most commonly used detection technology is qPCR, but this technology cannot achieve accurate quantification, and false negative and false positive results may occur in actual work, which brings great inconvenience to the epidemic prevention work of African swine fever

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