Method for preparing astaxanthin oil from Haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin oil, which is applied in the direction of organic chemistry, etc., can solve the problems of low production efficiency, complex process, many organic solvents required for adsorption, elution, and regeneration, etc. Filter rate, the effect of removing interference

Active Publication Date: 2022-04-26
YUNNAN AIERKANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The column chromatography system requires many organic solvents for adsorption, elution, and regeneration, and the process is complicated
and low production efficiency

Method used

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  • Method for preparing astaxanthin oil from Haematococcus pluvialis

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0036] Get 180kg of fresh Haematococcus pluvialls, with a water mass percentage of 55%, which is equivalent to 81.0kg of Haematococcus pluvialls dry base, and a mass percentage of astaxanthin of 4.54%, and put it into 270kg of 1.5mol / L dilute sulfuric acid solution. Haematococcus pluvialis has a mass percentage of 18.0% on a dry basis, stirs and slurries, heats up to 65°C and maintains the temperature, continues stirring to break the wall, breaks the wall for 25min, adds 22.0kg of calcium carbonate for neutralization, and the neutralization end point is PH6.0, transferred to the plate and frame filter press to obtain 201.6kg broken wall Haematococcus pluvialis cake, filtration rate 0.15m3 / (m2 h), sampling inspection, the water content mass percentage of Haematococcus pluvialls broken wall cake was 37.5 %, the wall breaking rate is 99.2%, the mass percentage of astaxanthin is 2.70%, and the recovery rate of astaxanthin is 92.5%.

[0037] Take 200kg of broken Haematococcus pluvi...

example 2

[0043] Get 170kg of fresh algae of Haematococcus pluvialls, with a water mass percentage of 53%, which is equivalent to 79.9 kg of Haematococcus pluvialls dry base, and a mass percentage of astaxanthin of 4.14%, and put it into 193 kg of 2.5mol / L dilute phosphoric acid solution, Haematococcus pluvialis has a mass percentage of 22.0% on a dry basis, stirs and slurries, heats up to 75°C and maintains the temperature, continues stirring to break the wall, breaks the wall for 60min, adds 23.0kg of calcium carbonate for neutralization, and the neutralization end point is PH6.5, transferred to the plate and frame filter press to obtain 203.3kg broken wall Haematococcus pluvialis cake, filtration rate 0.17m3 / (m2 h), sampling inspection, the water content mass percentage of Haematococcus pluvialls broken wall cake was 38.5 %, the wall breaking rate is 99.5%, the mass percentage of astaxanthin is 2.45%, and the recovery rate of astaxanthin is 92.5%.

[0044] Take 200kg of broken Haemat...

example 3

[0050] Get the fresh algae 188kg of Haematococcus pluvialls, the water mass percentage 56%, be equivalent to Haematococcus pluvialls dry base 80.84kg, astaxanthin mass percentage 4.36%, drop into the dilute sulfur-phosphorus mixed acid (sulfuric acid and 2.0mol / L of 216kg) Phosphoric acid (mass ratio: 1:1) solution, Haematococcus pluvialis in the algae pulp is 20.0% by mass percentage on a dry basis, stirred and pulped, heated to 70°C and maintained at this temperature, continued to stir to break the wall, break the wall 40min, add 22.0kg of calcium carbonate for neutralization, the neutralization end point is PH6.2, transfer to plate and frame filter press to obtain 203.5kg broken Haematococcus pluvialis cake, filtration rate 0.18m3 / (m2 h), sampling According to the detection, the water mass percentage of the broken wall cake of Haematococcus pluvialis is 36.5%, the wall breaking rate is 99.5%, the mass percentage of astaxanthin is 2.55%, and the recovery rate of astaxanthin i...

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Abstract

The invention discloses a method for preparing astaxanthin oil from Haematococcus pluvialls, in particular a method for preparing astaxanthin oil from Haematococcus pluvialls by alcohol extraction and purification, comprising the following steps: A) taking Put Haematococcus pluvialis into dilute acid solution, stir to break the wall, add calcium carbonate to neutralize, press filter to obtain the broken wall Haematococcus pluvialls cake; B) take the broken wall Haematococcus pluvialls cake, put it into alcohol, Stir and extract, centrifuge to obtain the extract; C) take the extract and distill under reduced pressure to obtain crude astaxanthin oil; D) take crude astaxanthin oil, put it into alcohol, stir and disperse, and settle and separate to obtain astaxanthin oil in the lower layer , desolvating under reduced pressure to obtain the finished product of astaxanthin oil. The invention not only has a high wall-breaking rate and a high recovery rate of astaxanthin, but also effectively removes the interference of chlorophyll and other β-carotenoids in astaxanthin, and effectively reduces the acid value of astaxanthin oil, petroleum ether insolubles, and heavy metal arsenic content, and greatly increase the content of astaxanthin in astaxanthin oil.

Description

technical field [0001] The invention relates to a method for preparing astaxanthin, in particular to a method for preparing astaxanthin oil by using Haematococcus pluvialis as a raw material through alcohol extraction and purification, and belongs to the technical field of beta-carotenoids. Background technique [0002] Astaxanthin is a fat-soluble β-carotenoid, which has a strong activity of scavenging free radicals and quenching reactive oxygen species, as well as good coloring properties. It has broad applications in food, aquaculture, medicine and cosmetics industries. application. [0003] Haematococcus pluvialis is currently the most important source of natural astaxanthin, and the content of astaxanthin in its chlamydospores reaches up to 7% of dry cell weight. Therefore, it is of great benefit to use Haematococcus pluvialis to produce astaxanthin. [0004] Astaxanthin exists in the form of esterification in Haematococcus pluvialis and coexists with algal oil of Haem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16
Inventor 张勇沈家会李顺友杨小萱
Owner YUNNAN AIERKANG BIOTECH
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