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Electrochemical logic gates based on glutathione, glutathione reductase and glucose-6-phosphate dehydrogenase

A technology of glutathione reductase and phosphate dehydrogenase, which is applied in the field of functional biomaterials and biosensing, can solve the problems of limited analysis and detection, and achieve the effects of fast analysis, less reagent consumption and simple operation

Active Publication Date: 2020-11-03
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past few decades, a variety of glutathione analysis and detection methods have emerged, mainly including colorimetry, fluorescence, mass spectrometry, high performance liquid chromatography, etc., and most of the analysis methods focus on the direct detection of GSH. Detection, while the associated glutathione reductase (GSR) uses reduced nicotinamide adenine dinucleotide phosphate (NADPH) to catalyze the reaction of oxidized GSH (GSSG) into reduced GSH (GSH), its Deletion makes cells more sensitive to oxidants and antibiotics) and glucose-6-phosphate dehydrogenase (G6PD, which catalyzes the dehydrogenation of glucose-6-phosphate to form 6-phosphogluconate and NADPH) assays are very limited

Method used

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  • Electrochemical logic gates based on glutathione, glutathione reductase and glucose-6-phosphate dehydrogenase
  • Electrochemical logic gates based on glutathione, glutathione reductase and glucose-6-phosphate dehydrogenase
  • Electrochemical logic gates based on glutathione, glutathione reductase and glucose-6-phosphate dehydrogenase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of GSH-Ag(I) CP and preparation of sensor

[0033] (1) Preparation of GSH-Ag(I)CP

[0034] Take 5 μL of 1 mM silver nitrate aqueous solution and 5 μL of 1 mM glutathione aqueous solution in turn, mix well and add phosphate buffer solution (10 mM, pH 7.0, Na 2 HPO 4 / NaH 2 PO 4 ) was prepared into a 60 μL solution, and the solution was slowly shaken at 37°C for 11 minutes to obtain GSH-Ag(I) CP. Before use, the CP solution was mixed with 60 μL 0.02% wt Nafion solution and allowed to stand for 12 minutes.

[0035] (2) Preparation of electrochemical biosensors

[0036] a. Polish the glassy carbon electrode (GCE, 3 mm in diameter) on the suede with Al2O3 powder with a particle size of 0.3 μm and 0.05 μm for 2 minutes. After polishing, place the electrode in an ultrasonic cleaner and use ultrapure water for ultrasonication Wash for 3 minutes, then blow dry with N2, and record it as GCE;

[0037] b. Using cyclic voltammetry, set the potential range...

Embodiment 2

[0039] Embodiment 2 Construction of Ag(I) "YES" logic gate

[0040] According to the preparation of the GSH-Ag(I) CP of Example 1, the sensor made by the polymer constructed when there is Ag(I) and no Ag(I) is compared to H 2 o 2 The electrochemical response of Ag(I) is constructed as the "YES" logic gate, as shown in the following table:

[0041]

[0042] The result is as figure 2 As shown, when it is proved that Ag(I) exists, for H 2 o 2 It has good electrochemical response and meets the characteristics of "YES" logic gate.

Embodiment 3

[0043] Embodiment 3 Construction of GSH "YES" logic gate

[0044] According to the preparation of the GSH-Ag(I) CP of Example 1, the sensor made by the polymer constructed when contrasting GSH exists and does not exist GSH is paired with H 2 o 2 The electrochemical response of GSH, construct the "YES" logic gate of GSH, as shown in the following table:

[0045]

[0046] The result is as image 3 As shown, when it is proved that GSH exists, for H 2 o 2 It has good electrochemical response and meets the characteristics of "YES" logic gate.

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Abstract

The invention discloses a preparation method of an electrochemical sensor based on silver ions and glutathione and logic gate application of the electrochemical sensor, and the preparation method comprises the following specific steps: firstly, preparing a graphite oxide modified electrode GO / GCE, then, reacting Ag (I) with GSH to generate a compound, and successfully preparing the sensor based onthe compound. generating NADPH by utilizing glucose 6-phosphoric acid (G6P) and reduced coenzyme II (NADP +) in an oxidation state under the catalysis of G6PD, wherein GSH can be generated by NADPH and GSSG under the catalysis of GSR, a coordination polymer is generated by utilizing the reaction of the generated GSH and Ag (I), and an obvious electrocatalytic signal is generated for H2O2. Based on the biological relationship between the two enzymes and GSH, different signal inputs and outputs are designed to construct four logic gates (YES, AND, AND-AND and AND-AND-AND), and a new thought isprovided for the diagnosis and treatment of GSH-related diseases. The sensor has the advantages of good specificity, high sensitivity, high detection speed, accurate and reliable result and low cost.

Description

technical field [0001] The present invention relates to electrochemical methods and their applications in logic gates, in particular to glutathione, glutathione reductase and glucose-6-phosphate dehydrogenase-related logic circuits based on the electrocatalytic activity of coordination polymers Design, which belongs to the field of functional biomaterials and biosensing technology. Background technique [0002] Logic gates are the basic building blocks of integrated circuits. Simple logic gates generally consist of transistors. The high and low levels representing the two signals can be combined by transistors in the integrated circuit to generate a high or low level signal. These two signals can be represented by "true" and "false" or 1 and 0 in binary, respectively. Among them, the binary structure requires fewer originals, and the operation rules are simple, which is convenient for logical operations. Boolean logic is usually used to represent a series of logical oper...

Claims

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Application Information

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IPC IPC(8): G01N27/27G01N27/327G01N27/30
CPCG01N27/27G01N27/3278G01N27/308
Inventor 张青青胡宇芳詹甜玉
Owner NINGBO UNIV
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