Kit for rapidly detecting malignant transformation of passage stem cells and application of kit

A kit and stem cell technology, applied in the field of stem cells, can solve problems such as difficult diagnosis, and achieve the effects of good accuracy, high sensitivity and simple operation

Pending Publication Date: 2020-11-13
成都睿杰森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with previous statistical methods, this method fully considers the methylation degree and influence of different genes, however, this method requires statistical analysis of a large number of samples, so it is difficult to apply to instant diagnosis of a single sample or a small number of samples

Method used

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  • Kit for rapidly detecting malignant transformation of passage stem cells and application of kit
  • Kit for rapidly detecting malignant transformation of passage stem cells and application of kit
  • Kit for rapidly detecting malignant transformation of passage stem cells and application of kit

Examples

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Embodiment 1

[0034] The Taq enzyme used in this example was purchased from Dalian Bao Biological Company (TaKaRa); the cell genome extraction kit was AxyPrepTM Multisource Genomic DNA Miniprep Kit (Axygen Scientific, Inc. CA, USA); the vulcanization kit was EZ DNA Methylation kit (Zymo Research, Orange, CA, USA); other reagents were domestic analytical reagents.

[0035] The stem cells used in this example are stem cells isolated from different cancer tissues.

[0036] The primers involved in this embodiment are:

[0037] Target gene P16 forward primer: TTATTAGAGGGTGGGGCGGATCGC

[0038] Target gene P16 reverse primer: GACCCCGAACCGCGACCGTAA

[0039] Target gene RASSF1A forward primer: GACCTCTGTGGCGACTTCATCTG

[0040] Target gene RASSF1A reverse primer: GACCTAGTCCTCGGGAGCTGTC

[0041] Target gene GSTP1 forward primer: TTCGGGGTGTAGCGGTCGTC

[0042] Target gene GSTP1 reverse primer: GCCCCAATACTAAATCACGACG

[0043] Target gene CDH1 forward primer: TTAGGTTAGAGGGTTATCGCGT

[0044] Target g...

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Abstract

The invention provides a kit for rapidly detecting malignant transformation of passage stem cells and application of the kit, belongs to the technical field of stem cells, the kit comprises primers corresponding to four target genes of P16, RASSF1A, GSTP1 and CDH1, and a reference gene beta-actin primer, the kit is applied to rapid detection of malignant transformation of passage stem cells, and has the advantages of simple operation, good accuracy and high sensitivity in detection of malignant transformation of stem cells.

Description

technical field [0001] The present invention is a divisional application with application number 201710483461.2. The filing date of the original application is 2017.06.23. The title of the invention is a kit for rapidly detecting the malignant transformation of passaged stem cells. [0002] The invention belongs to the technical field of stem cells, and in particular relates to a kit for rapidly detecting the malignant transformation of passaged stem cells and its application. Background technique [0003] Currently, stem cell development is in full swing. The development of stem cell therapy will help humans realize their dreams of repairing trauma and pathological tissues and curing terminal diseases, and is expected to solve many major medical problems facing humans. [0004] The mechanism of tumorigenesis and the origin of cells are hotspots and difficulties in oncology research. The theory of cancer stem cells holds that tumors originate from the malignant transformat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6858
CPCC12Q1/6858C12Q1/6886C12Q2600/154C12Q2600/166C12Q2531/113C12Q2545/101
Inventor 喻堃徐国锋
Owner 成都睿杰森生物科技有限公司
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