Flow cytometer fluorescence collection lens and light path system thereof
A flow cytometer and fluorescence technology, which is applied in the field of flow cytometry detection equipment, can solve the problems of poor compatibility and high cost of the collection lens, achieve good chromatic aberration correction capabilities, save coating costs, and save instrument costs Effect
Active Publication Date: 2020-11-13
北京指真生物科技有限公司
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Problems solved by technology
[0007] Because flow cytometers have strict requirements on the technical specifications of the collection lens, the existing collection lens generally requires targeted design, which is difficult to obtain directly on the market, and the compatibility of the collection lens is poor and the cost is high
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[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0031] On the one hand, if figure 1 As shown, the embodiment of the present invention discloses a fluorescence collection lens of a flow cytometer, and the lens includes: a plano-convex lens 11, a meniscus lens 12, a double-convex lens 13, a first doublet lens 14 and a second doublet lens along the propagation direction of the optical path. A cemented lens 15, and a plano-convex lens 11, a meniscus lens 12, a biconvex lens 13, a first doublet lens 14 and a sec...
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The invention discloses a flow cytometer fluorescence collection lens and an optical path system thereof, the lens realizes a large numerical aperture through the combination of a plano-convex lens, apositive meniscus lens, a biconvex lens and two doublet lenses, and can collect fluorescence scattering light in a half-angle range of 45 degrees; the lens meets the requirement that the working distance is larger than 2 mm, and can be compatible with a flow chamber used on a common flow cytometer; good chromatic aberration correction capability is realized on the wavelength range of 400 to 820nm; parallel light can be output, the beam diameter is within 5 mm, the lens meets the use requirement that the dichroic mirror and the color filter are sensitive to the incident angle of the light beam, meanwhile, the color filter and the dichroic mirror achieve light beam transmission with the small size, the coating cost and the instrument cost are greatly saved, and the lens is simple, easy to obtain and suitable for common flow cytometry.
Description
technical field [0001] The invention relates to the technical field of detection equipment using flow cytometry, and more specifically relates to a fluorescence collection lens of a flow cytometer and an optical path system thereof. Background technique [0002] Currently, in flow cytometry, cells pass through the instrument and are detected at a rate of 10,000 cells per second (or more), providing a wide range of statistical capabilities for cell biology. The cells pass through the laser beam of the instrument to generate specific fluorescent signals, which are then collected by the collection lens, and then further divided by dichroic mirrors and color filters according to the wavelength bands, and then converged to the photodetector to complete the signal reception. Existing flow cytometry fluorescence collection lenses need to meet the following important technical indicators: [0003] First, because the larger the numerical aperture (Numerical aperture, NA) of the coll...
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Login to View More IPC IPC(8): G01N15/14G01N21/01G01N21/64G02B7/02G02B13/00G02B13/18
CPCG01N15/1434G01N21/6458G01N21/01G02B13/006G02B13/18G02B7/025G01N2021/6478G01N2021/0112
Inventor 杨波马永波秦晓琨李根
Owner 北京指真生物科技有限公司