A flow cytometer fluorescence collection lens and its optical path system
A flow cytometer and fluorescence technology, which is applied in the field of flow cytometry detection equipment, can solve the problems of high cost, poor compatibility and use performance of the collection lens, etc., and achieve the advantages of saving coating costs, good color correction ability, and saving instruments cost effect
Active Publication Date: 2022-03-25
北京指真生物科技有限公司
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Problems solved by technology
[0007] Because flow cytometers have strict requirements on the technical specifications of the collection lens, the existing collection lens generally requires targeted design, which is difficult to obtain directly on the market, and the compatibility of the collection lens is poor and the cost is high
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[0030] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0031] On the one hand, if figure 1 As shown in the figure, the embodiment of the present invention discloses a fluorescence collection lens for a flow cytometer. The lens includes a plano-convex lens 11 , a meniscus lens 12 , a biconvex lens 13 , a first doublet lens 14 and a second doublet lens along the optical path propagation direction. A cemented lens 15, and the plano-convex lens 11, the meniscus lens 12, the biconvex lens 13, ...
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Abstract
The invention discloses a flow cytometer fluorescence collection lens and its optical path system. The lens realizes a relatively large numerical aperture through the combination of a plano-convex lens, a positive meniscus lens, a double-convex lens and two doublet lenses, and can Collect fluorescence scattered light within the range of half-angle 45°; and the lens meets the requirement that the working distance is greater than 2mm, and is compatible with the flow chambers used in common flow cytometers; it has a wavelength range of 400-820nm Good chromatic aberration correction ability, and can output parallel light, the beam diameter is within 5mm, which meets the requirements of the use of dichroic mirrors and color filters that are sensitive to the incident angle of the beam, and at the same time, makes the color filters and dichroic mirrors The beam is cleared with a smaller size, which greatly saves the cost of coating and instrumentation. The lens is simple and easy to obtain, and is applicable to common flow cytometers.
Description
technical field [0001] The invention relates to the technical field of detection equipment using flow cytometry technology, and more particularly to a fluorescence collection lens of a flow cytometer and an optical path system thereof. Background technique [0002] Currently, in flow cytometry, cells are passed through the instrument and detected at a rate of 10,000 cells per second (or more), providing a wide range of statistical capabilities for cell biology. The cells can generate specific fluorescence signals through the laser beam of the instrument, and then collect them through the collection lens, and then further divide the fluorescence according to the wavelength band through the dichroic mirror and color filter, and then converge to the photodetector to complete the signal reception. Existing flow cytometer fluorescence collection lenses need to meet the following important technical indicators: [0003] First, because the larger the numerical aperture (NA) of the...
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IPC IPC(8): G01N15/14G01N21/01G01N21/64G02B7/02G02B13/00G02B13/18
CPCG01N15/1434G01N21/6458G01N21/01G02B13/006G02B13/18G02B7/025G01N2021/6478G01N2021/0112
Inventor 杨波马永波秦晓琨李根
Owner 北京指真生物科技有限公司