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A kind of method for preparing low pesticide residue plateau anthocyanin grape seed extract

A grape seed and extract technology, which is applied in the field of preparation of grape seed extract with low pesticide residues and plateau anthocyanins, can solve the problem of excessive pesticide residues and other issues

Active Publication Date: 2022-08-05
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved in the present invention is to provide a method for preparing grape seed extracts with low pesticide residues and high proanthocyanidins, which can solve the problem of excessive pesticide residues in grape seed extracts, and can also ensure the yield of proanthocyanidins and polyphenols

Method used

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  • A kind of method for preparing low pesticide residue plateau anthocyanin grape seed extract

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Experimental program
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Effect test

Embodiment 1

[0029] A method for preparing low pesticide residue grape seed extract, comprising the steps of:

[0030] S1. Cleaning: 1000 kilograms of grape seeds are treated with negative pressure pulse device, the pressure is 96kPa, the temperature is 55℃, and the treatment is 1h;

[0031] S2, extraction: add the cleaned grape seeds into the extractor, add 4000L of 60% ethanol solvent to extract at 50°C, and extract three times to obtain an extract;

[0032] S3, centrifugation: the extract in step S2 is concentrated under reduced pressure at 70°C, the vacuum degree is -0.09MPa, the concentrated liquid is centrifuged under the condition of 4000r / min, and the supernatant is collected;

[0033] S4. Chromatography: the supernatant obtained in step S3 is adsorbed by LSA-12 resin and analyzed with 70% ethanol to obtain a solution;

[0034] S5, activated carbon treatment: add 6 kilograms of activated carbon to the analytical solution obtained in step S4, wherein, activated carbon from fruit sh...

Embodiment 2

[0037] A method for preparing low pesticide residue grape seed extract, comprising the steps of:

[0038] S1. Cleaning: 1000 kilograms of grape seeds are treated with a negative pressure pulse device, the pressure is 128kPa, the temperature is 45°C, and the treatment is 1.5h;

[0039] S2, extraction: add the cleaned grape seeds into the extractor, add 5000L of 70% ethanol solvent to extract at 50°C, and extract three times to obtain an extract;

[0040] S3, centrifugation: the crude extract of grape seed in step S2 is concentrated under reduced pressure at 70°C, the vacuum degree is -0.07MPa, the concentrated liquid is centrifuged under the condition of 4000r / min, and the supernatant is collected;

[0041] S4, chromatography: the supernatant obtained in step S3 is adsorbed on LX-1600 resin, and analyzed with 75% ethanol;

[0042] S5. Activated carbon treatment: adding 6.5 kg of activated carbon to the analytical solution obtained in step S4, wherein the nutshell activated car...

Embodiment 3

[0045] A method for preparing low pesticide residue grape seed extract, comprising the steps of:

[0046] S1. Cleaning: 1000 kilograms of grape seeds are treated with a negative pressure pulse device, the pressure is 128kPa, the temperature is 55°C, and the treatment is 1.5h;

[0047] S2. Extraction: add the cleaned grape seeds into the extractor, add 5000L of 70% ethanol solvent to extract at 60°C, and extract three times to obtain an extract;

[0048] S3, centrifugation: the crude extract of grape seed in step S2 is concentrated under reduced pressure at 70°C, the vacuum degree is -0.07MPa, the concentrated liquid is centrifuged under the condition of 4000r / min, and the supernatant is collected;

[0049] S4, chromatography: the supernatant obtained in step S3 is adsorbed on LX-1600 resin, and desorbed with 75% ethanol;

[0050] S5, activated carbon treatment: add 6.5 kg of nutshell activated carbon: wood activated carbon=1:2 activated carbon to the analytical solution obtai...

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Abstract

The invention discloses a method for preparing a low-pesticide high-altitude anthocyanin grape seed extract, comprising the following steps: S1, cleaning: cleaning the grape seeds with a negative pressure pulse device; S2, extracting: adding the cleaned grape seeds into an extractor Add a solvent for extraction to obtain an extract; S3, centrifugation: concentrate the extract in step S2 to obtain a concentrate, and centrifuge the concentrate to collect the supernatant; S4, chromatography: pass the supernatant obtained in step S3 through a macropore Adsorption resin column, adsorption analysis to obtain analysis solution; S5, activated carbon treatment: adding activated carbon to the analysis solution obtained in step S4, stirring and adsorption, and filtering to obtain filtrate; S6, drying: drying the filtrate in step S5 to obtain low pesticide residue grape seed extract The invention can solve the problem of excessive pesticide residues in the grape seed extract, and can ensure the yield of proanthocyanidins and polyphenols at the same time.

Description

technical field [0001] The invention relates to a method for preparing a grape seed extract with low pesticide residues and plateau anthocyanin, belonging to the field of plant extracts. Background technique [0002] Grape seed extract has certain ability of scavenging free radicals and anti-oxidation. The proanthocyanidins extracted from grape seeds with high anti-oxidative activity are the water-soluble substances with the strongest anti-oxidative ability in natural products so far. The human body cannot synthesize proanthocyanidins, but proanthocyanidins can effectively scavenge free radicals, and have strong anti-aging, beauty, and immune-enhancing effects. [0003] The current extraction methods include steam distillation, ultrasonic extraction and supercritical fluid extraction, etc., all of which have the problem of excessive pesticide residues. [0004] Patent 2009100386203 discloses a method for removing pesticide residues in tea leaves. It utilizes ozone water or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/87A61P39/06A61P37/04A61P17/00A61K131/00
CPCA61K36/87A61P39/06A61P37/04A61P17/00A61K2236/13A61K2236/333A61K2236/39A61K2236/55
Inventor 蔡亚园安晓东连运河高伟杨丽微
Owner CHENGUANG BIOTECH GRP CO LTD