Ascomycete fermentation method
A fungal, wide-ranging technology for use in fermentation, biochemical equipment and methods, fungi, etc.
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specific Embodiment approach A
[0046] A method for fermentation of Aureobasiaceae, comprising the steps of:
[0047] a) providing a medium having a dry matter content selected from the range of 0.1 to 0.3 wt.-%, and containing less than 0.1 wt.-% of an organic nitrogen source;
[0048] b) with at least one fungus belonging to the species Aureobasidium pullulans selected from 0.01 g CDW / L to 50g CDW Inoculate the culture medium at an inoculation density in the / L range;
[0049] c) adding at least one carbon source in a concentration of 1 to 20 wt.-%;
[0050] d) mixing the culture medium and at least one fungus according to step d) for a period of 24 to 400 hours;
[0051] e) removing 30 to 90 wt.-% of the culture medium and at least one fungus;
[0052] f) adding 30 to 90 wt.- of the medium as defined in step a) additionally comprising a carbon source;
[0053] g) repeat steps c) to f) 2 to 100 times;
[0054]wherein at least one of steps c) to g) is carried out at a relative gas content selected fr...
specific Embodiment approach B
[0056] A method for fermentation of Aureobasiaceae, comprising the steps of:
[0057] a) providing a medium having a dry matter content selected from the range of 0.1 to 0.3 wt.-%, and containing less than 0.1 wt.-% of an organic nitrogen source;
[0058] b) with at least one fungus belonging to the species Aureobasidium pullulans selected from 0.01 g CDW / L to 50g CDW Inoculate the culture medium at an inoculation density in the / L range;
[0059] c) adding sucrose at a concentration of 1 to 20 wt.-%;
[0060] d) mixing the culture medium and at least one fungus according to step d) for a period of 24 to 400 hours;
[0061] e) removing 30 to 90 wt.-% of the culture medium and at least one fungus;
[0062] f) adding 30 to 90 wt.- of the medium as defined in step a) additionally comprising a carbon source;
[0063] g) repeat steps c) to f) 2 to 100 times;
[0064] wherein at least one of steps c) to g) is carried out at a relative gas content selected from the range of 0....
specific Embodiment approach C
[0066] A method for fermentation of Aureobasiaceae, comprising the steps of:
[0067] a) providing a medium having a dry matter content selected from the range of 0.1 to 0.3 wt.-%, and containing less than 0.1 wt.-% of an organic nitrogen source;
[0068] b) with at least one fungus belonging to the species Aureobasidium pullulans selected from 0.01 g CDW / L to 50g CDW Inoculate the culture medium at an inoculation density in the / L range;
[0069] c) adding sucrose at a concentration of 1 to 20 wt.-%;
[0070] d) mixing the culture medium and at least one fungus according to step d) for a period of 24 to 400 hours;
[0071] e) removing 30 to 90 wt.-% of the culture medium and at least one fungus;
[0072] f) adding 30 to 90 wt.- of the medium as defined in step a) additionally comprising a carbon source;
[0073] g) repeat steps c) to f) 2 to 100 times;
[0074] Wherein at least one of steps c) to g) is carried out at a relative gas content selected from the range of 0....
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