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Anti-human SIRP alpha monoclonal antibody and application thereof

A monoclonal antibody and antibody technology, applied in the direction of antibodies, anti-animal/human immunoglobulins, applications, etc., can solve the problems of limited specificity of anti-SIRPα antibodies, negative effects on T cell proliferation and recruitment, and achieve novel sequences Effect

Active Publication Date: 2020-11-27
BIOSION INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Binding to SIRPγ expressed on T cells may negatively affect T cell proliferation and recruitment
Other anti-SIRPα antibodies also have limited specificity

Method used

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  • Anti-human SIRP alpha monoclonal antibody and application thereof
  • Anti-human SIRP alpha monoclonal antibody and application thereof
  • Anti-human SIRP alpha monoclonal antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 obtains the mouse monoclonal antibody of specific anti-SIRPα by fusion hybridoma technology

[0042] 1.1 Animal immunity

[0043] Mice were immunized according to the general method in the literature (E Harlow, D. Lane, Antibody: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1998). 免疫原为C端含有人IgG1 Fc标签的重组人SIRPα(氨基酸Met 1-Arg 370)蛋白(公司自产,所用质粒为CD172-ECD-Fc;载体为pCP;插入氨基酸序列为MEPAGPAPGRLGPLLCLLLAASCAWSGVAGEEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER)。 Use recombinant human SIRPα protein with his tag (produced by the company itself, the plasmid used is SRPA-ECD-His; the vector is pCMV-C-His; the amino acid sequence inserted between EcoR I an...

Embodiment 2

[0057] Example 2 In vitro analysis method for measuring the functional activity of SIRPα monoclonal antibody

[0058] 2.1 Determination of antibody binding ability based on capture ELSIA

[0059] Prepare goat anti-mouse IgG F(ab')2-specific secondary antibody (Jackson ImmunoResearch Laboratories, Inc., Cat#115-005-072) in 1xPBS to a final concentration of 2 μg / ml, add 100 μl / well to 96-well ELISA plates were coated at 37°C for 2 hours. After coating, wash the plate once with 1xPBST, add 200 μl / well of PBST solution containing 5% skimmed milk powder and place at 37 degrees Celsius for 2 hours to block. Wash the plate again, add 100 μl / well of diluted antibody solution and Benchmark (that is, KWAR23 antibody, produced by the company, and its heavy chain amino acid is

[0060] EVQLQQSGAELVKPGASVKLSCTASGFNIKDYYIHWVQQRTEQGLEWIGRIDPEDGETKYAPKFQDKATITADTSSNTAYLHLSSLTSEDTAVYYCARWGAYWGQGTLVTVSAASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSN...

Embodiment 3

[0081] Example 3 Binding Activity of Anti-SIRPα Mouse Monoclonal Antibody

[0082] According to the analysis method described in Example 2, the evaluation of the binding activity of the SIRPα antibody is summarized in Table 3 below. Among them, SIRPα Benchmark was used as a control. It can be seen from Table 3 that the binding activity of the anti-human SIRPα monoclonal antibody of the present invention to human SIRPα antigen is slightly better than that of Benchmark, and can cross-react with SIRPβ antigen at the same time.

[0083] Table 3 Binding activity of SIRPα antibodies

[0084]

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Abstract

The invention belongs to the technical field of biology, relates to an anti-human SIRP alpha monoclonal antibody and application thereof, and also provides a coding nucleic acid molecule, an expression vector and a host cell of the antibody, and a method for expressing the antibody. The invention also provides an antibody immunoconjugates comprising the antibody of the invention, bispecific molecules, a chimeric antigen receptor, a pharmaceutical composition, and a diagnosis and treatment method. According to the invention, the human SIRP alpha monoclonal antibody can be specifically recognized, the affinity of the antibody and the human SIRP alpha is higher than that of an existing anti-human SIRP alpha monoclonal antibody, and the sequence is novel.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a high-affinity and functional anti-human SIRPα monoclonal antibody or antibody fragment. The present invention also provides the encoding nucleic acid molecules of the antibody, expression vectors, host cells, and methods for expressing the antibody. Also provided are antibody immunoconjugates, bispecific molecules, chimeric antigen receptors, pharmaceutical compositions, and diagnostic and therapeutic methods comprising antibodies of the invention. Background technique [0002] Signal regulatory protein α (Signal regulatory protein α), also known as SHPS-1 or SIRPα, is a typical inhibitory immune receptor in the SIRP family, mainly expressed in myeloid cells such as macrophages and dendritic cells, And the surface of nerve cell membrane, while other cells in the body express less (Kharitonenkov, A. et al. (1997). "Afamily of proteins that inhibit signaling through tyrosine kinase rec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C12N15/13A61K39/395A61P35/00
CPCC07K16/2896A61P35/00C07K2317/51C07K2317/515C07K2317/56C07K2317/565C07K2317/33C07K2317/76C07K2317/92A61K2039/505
Inventor 陈明久
Owner BIOSION INC
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