Cooling and rewarming system for vitrification preservation of biological material
A biological material and vitrification technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of untraceable preservation process, low preservation efficiency, and difficult operation, so as to achieve traceability, reduce Small safety hazards, reduce the effect of operation difficulty
Active Publication Date: 2020-12-04
UNIV OF ELECTRONICS SCI & TECH OF CHINA
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Problems solved by technology
[0003] In order to overcome the problems of poor consistency, difficult operation, low storage efficiency, and untraceable storage process in the existing low-temperature storage methods for biological materials, the present invention provides a cooling and rewarming system for vitrification storage of biological materials
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[0030] The present invention will be further described through the embodiments below in conjunction with the accompanying drawings.
[0031] see figure 1 , a cooling and reheating system for vitrification preservation of biological materials, including a reheating and cooling workbench mechanism, a gas source tank 1, a cooling working medium container 2, a rewarming working medium container 3, an external liquid storage tank 4, and an external liquid supply tank 5 and electric air pump 20.
[0032] see figure 2 , the reheating and cooling workbench mechanism includes a fluidic mechanism, a clamping mechanism, a controller and a workbench casing 81 .
[0033] The jet flow mechanism includes a pair of jet flow generating mechanisms, that is, an upper jet flow generating mechanism and a lower jet flow generating mechanism.
[0034] The upper jet generating mechanism includes an upper jet generator 813 , an upper clamping plate 83 and a transition plate 85 . The transition pl...
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The invention relates to a cooling and rewarming system for vitrification preservation of a biological material, and belongs to the technical field of preservation of biological materials. The systemis characterized in that a rewarming and cooling workbench mechanism, an air source tank, a cooling working medium container, a rewarming working medium container, an outer liquid storage tank, an outer liquid supply tank, an electric air pump and the like form a loop; the rewarming and cooling workbench mechanism comprises a jet mechanism, a clamping mechanism, a controller and a workbench shell.During working, the air source tank is opened, high-pressure gas in the air source tank is introduced into the cooling working medium container or the rewarming working medium container to press outa working medium, and the working medium flows into a jet flow generator in the rewarming and cooling workbench mechanism through a second three-way valve, so that working medium undergoes high-speedjet flow impinging to a sample box, and liquid nitrogen jet cooling or warm water jet rewarming operation is symmetrically carried out on the sample box. According to the invention, the problem of poor consistency caused by manual operation is reduced, and the storage efficiency is improved.
Description
technical field [0001] The invention belongs to the technical field of preservation of biological materials, and in particular relates to a cooling or rewarming system for low-temperature vitrification preservation of biological materials such as cells, tissues and organs. Background technique [0002] Low temperature preservation is currently the only effective long-term preservation method for many biological materials, and vitrification preservation is one of the important methods, which avoids the traditional slow-speed preservation by transforming liquid substances into a highly viscous amorphous state (glass state). The dehydration process of the cryopreservation method prevents the internal tissues of biological samples from producing (or only produces a small amount of) ice crystals during the preservation process, so that the cells are less damaged by low temperature. The key to achieving vitrification is to quickly cool down and rewarm biological samples. The exist...
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IPC IPC(8): A01N1/02
CPCA01N1/0242A01N1/0252
Inventor 周晓明冷顺刘杰刘浩
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA