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A method and device suitable for photoacoustic/fluorescence dual-mode imaging of abdominal organs of animals

A dual-mode imaging technology for visceral organs, which is applied in the fields of biological sciences and medical devices and appliances, can solve problems such as visceral visceral vibrations in the abdominal cavity, and achieve good biocompatibility, excellent light transmittance, and good application prospects

Active Publication Date: 2021-10-22
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a method and device suitable for photoacoustic / fluorescence dual-modal imaging of abdominal internal organs of animals. The abdominal window material sheet that meets the requirements of photoacoustic microscopic imaging and fluorescent imaging is selected at the same time. The idea of ​​assembling into a "drawer type" can further solve the problem of visceral visceral vibration caused by animal breathing and heartbeat jitter, and lay a foundation for the imaging research of abdominal visceral organs

Method used

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  • A method and device suitable for photoacoustic/fluorescence dual-mode imaging of abdominal organs of animals
  • A method and device suitable for photoacoustic/fluorescence dual-mode imaging of abdominal organs of animals
  • A method and device suitable for photoacoustic/fluorescence dual-mode imaging of abdominal organs of animals

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Experimental program
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Embodiment 1

[0044] This embodiment provides a photoacoustic / fluorescence dual-mode imaging device suitable for the abdominal organs of animals, such as image 3 As shown, it includes a belly window ring 1, the top surface of the belly window ring 1 is bonded with a circular transparent cover sheet 2, the cover sheet 2 completely covers the window of the belly window ring 1, and the cover sheet 2 and the belly window ring 1 The inner wall is interference fit and bonded by biological tissue glue;

[0045] The bottom surface of the ventral window ring 1 is bonded with a semicircular transparent support piece 3, the arc edge of the support piece 3 is bonded to the inner wall of the ventral window ring 1 through biological tissue glue, and the support piece 3 covers the ventral window ring 1 50% of the window area;

[0046]The supporting sheet 3 and the cover sheet 2 are made of acrylic material with a thickness of 1.0mm.

Embodiment 2

[0048] This embodiment provides a photoacoustic / fluorescence dual-mode imaging device suitable for the abdominal organs of animals, as shown in Figures 1 and 2, including a ventral window ring 1, and the top surface of the ventral window ring 1 is glued with biological tissue Connected with a circular transparent cover 2, the cover 2 is bonded to the top surface of the ventral ring 1, and the cover 2 completely covers the window of the ventral ring 1;

[0049] The bottom surface of the abdominal window ring 1 is bonded with a semicircular transparent support piece 3, and the support piece 3 is bonded to the top surface of the abdominal window ring 1 by biological tissue glue, and the support piece 3 covers the window area of ​​the abdominal window ring 1. 50%;

[0050] The supporting sheet 3 and the cover sheet 2 are made of acrylic material with a thickness of 1.0 mm. The diameters of the cover sheet 2 and the supporting sheet 3 are both smaller than the outer diameter of th...

Embodiment 3

[0052] This embodiment provides a photoacoustic / fluorescence dual-mode imaging method suitable for abdominal organs of animals, the steps include:

[0053] 1) Glue a semicircular acrylic or resin support on the bottom of the ventral window ring to make the ventral window ring into a drawer-like shape;

[0054] 2) Adhere a circular acrylic or resin material with the same diameter as the inner diameter of the abdominal window ring on the surface of the abdominal window, so that it can be perfectly fitted and sealed, and a photoacoustic / fluorescent light suitable for the internal organs of the animal abdominal cavity with the same structure as in Example 1 is obtained. Dual modality imaging device.

[0055] 3) Disinfect and sterilize the device obtained in step 2) by ultraviolet irradiation or wiping with alcohol;

[0056] 4) use the device after step 3) for liver window surgery in C57BL / 6 mice;

[0057] 5) Confocal and photoacoustic microscopy imaging of C57BL / 6 mice treated i...

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Abstract

The present invention provides a photoacoustic / fluorescence dual-mode imaging method and device suitable for animal abdominal viscera, which consists of abdominal window ring and acrylic material or resin material (such as: CR-39, PC, PMMA, etc.) through ingenious design Assembled, acrylic and resin materials (such as: CR‑39, PC, PMMA) have excellent light and sound permeability, and the photoacoustic / fluorescence dual-mode imaging method of the whole abdominal viscera has good biophase Capacitive, it can be used for both fluorescence confocal and photoacoustic imaging for long-term (>20 days) dynamic study of internal organs. The acrylic or resin sheet used at the same time can stabilize the abdominal viscera of the animal in vivo, and prevent the viscera of the abdominal cavity of the animal from being affected by the vibration of breathing and heartbeat on the stability of the imaging focal plane.

Description

technical field [0001] The invention belongs to the technical fields of biological sciences and medical instruments and appliances, and in particular relates to a photoacoustic / fluorescence dual-mode imaging method and device suitable for abdominal internal organs of animals. Background technique [0002] As a newly developed imaging technology, photoacoustic microscopy uses ultrasound as the medium, combining the advantages of high contrast of optical imaging and high penetration of ultrasound imaging. Fluorescence confocal imaging has high imaging contrast and high sensitivity, but it is limited by the serious scattering of light in biological tissues, and it is difficult to break through the hard limit of light transmission. Therefore, it is very necessary to combine the two and learn from each other's strengths for the study of the internal organs of the abdominal cavity of animals. However, the transmission of ultrasound is limited by glass. In actual photoacoustic mic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B5/00
CPCA61B5/0071A61B5/0095A61B2503/40
Inventor 张智红邓得强骆清铭
Owner HUAZHONG UNIV OF SCI & TECH