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Culture medium for agriophyllum squarrosum tissue culture, adventitious bud induction method and adventitious root induction method

A technology of tissue culture and culture medium, applied in the field of culture medium of Sapona tissue culture, to achieve the effects of high rooting efficiency, improved induction efficiency and high induction rate

Active Publication Date: 2020-12-08
NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is currently a lack of a complete tissue culture system for Sapon

Method used

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  • Culture medium for agriophyllum squarrosum tissue culture, adventitious bud induction method and adventitious root induction method
  • Culture medium for agriophyllum squarrosum tissue culture, adventitious bud induction method and adventitious root induction method

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The method that the induced saponica callus that the present embodiment provides forms adventitious buds is as follows:

[0037] (1) Callus induction culture:

[0038] In each embodiment of the present invention, hypocotyls or cotyledons are used as explants, and the reference application number 201611144975.7, the method of the Chinese invention patent named Shami callus tissue and its induction and proliferation method, is used to obtain the Sapona callus organization (see figure 1 In A and B), the callus medium used includes: MS+0.5mg / L 6-BA+3.0mg / L 2,4-D+0.70% agar+2.0% sucrose, pH 5.8-6.0.

[0039] (2) Adventitious bud induction culture

[0040] Inoculate the saponin callus piece that step (1) obtains in the adventitious bud induction medium and carry out bud induction culture;

[0041] The adventitious bud induction medium used included: MS+2 mg / L 6-BA+1 mg / L NAA+0.70% agar+2.0% sucrose.

[0042] Conditions for adventitious bud induction culture: humidity: 40%...

Embodiment 2

[0045] The method that the induced saponica callus that present embodiment provides forms adventitious root is as follows:

[0046] The callus of Example 1 was inoculated in the adventitious root induction medium for root induction culture; the adventitious root induction culture used included: 1 / 2MS+3mg / L IBA+0.70% agar+2.0% sucrose, pH 5.8-6.0;

[0047] Conditions for adventitious root induction culture: temperature: 22-28°C, humidity: 40%, time: 2-6 weeks, photoperiod: 16h light / 8h dark.

[0048] adventitious roots figure 2 Middle D, the arrow points to the adventitious root formed after 6 weeks of induction.

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Abstract

The invention discloses a culture medium for agriophyllum squarrosum tissue culture, an adventitious bud induction method and an adventitious root induction method, and relates to the technical fieldof tissue culture. The culture medium disclosed by the invention comprises an adventitious bud induction culture medium and an adventitious root induction culture medium. The culture medium disclosedby the invention can induce agriophyllum squarrosum calluses to differentiate to form adventitious buds and induce the calluses to generate adventitious roots. The culture medium for the agriophyllumsquarrosum tissue culture, the adventitious bud induction method and the adventitious root induction method lay a foundation for further establishing and perfecting a tissue culture system of agriophyllum squarrosum.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a culture medium used for tissue culture of Sapona, and a method for inducing adventitious buds and adventitious roots. Background technique [0002] Agriophyllum squarrosum (scientific name: Agriophyllum squarrosum (L.) Moq.), also known as Agriophyllum squarrosum (L.) Moq., is a plant of the genus Agriophyllum squarrosum in Chenopodiaceae. Branches, leaves sessile, lanceolate, lanceolate strips or strips, spikes dense, oval or elliptic, sessile, tepals membranous. Stamens cone-shaped, membranous, anthers ovoid. The fruit is oval or elliptic, and the seeds are nearly round, smooth, sometimes with light brown spots. Widely distributed in the desert areas of Northwest my country, North China and Northeast China provinces, as well as parts of Henan, Hebei, Shanxi and Tibet, Mongolia, former Soviet Union Siberia and Central Asia are also distributed. [0003] Sand rice has...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张继伟赵昕陈国雄赵鹏善
Owner NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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