Culture medium for agriophyllum squarrosum tissue culture, adventitious bud induction method and adventitious root induction method

A technology of tissue culture and culture medium, applied in the field of culture medium of Sapona tissue culture, to achieve the effects of high rooting efficiency, improved induction efficiency and high induction rate

Active Publication Date: 2020-12-08
NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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AI-Extracted Technical Summary

Problems solved by technology

[0004] However, there is currently a lack o...
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Abstract

The invention discloses a culture medium for agriophyllum squarrosum tissue culture, an adventitious bud induction method and an adventitious root induction method, and relates to the technical fieldof tissue culture. The culture medium disclosed by the invention comprises an adventitious bud induction culture medium and an adventitious root induction culture medium. The culture medium disclosedby the invention can induce agriophyllum squarrosum calluses to differentiate to form adventitious buds and induce the calluses to generate adventitious roots. The culture medium for the agriophyllumsquarrosum tissue culture, the adventitious bud induction method and the adventitious root induction method lay a foundation for further establishing and perfecting a tissue culture system of agriophyllum squarrosum.

Application Domain

Technology Topic

Culture mediumsAgriophyllum squarrosum +5

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  • Culture medium for agriophyllum squarrosum tissue culture, adventitious bud induction method and adventitious root induction method
  • Culture medium for agriophyllum squarrosum tissue culture, adventitious bud induction method and adventitious root induction method

Examples

  • Experimental program(2)
  • Comparison scheme(2)

Example Embodiment

[0035] Example 1
[0036] The method for inducing Saponica callus to form adventitious buds provided by the present embodiment is as follows:
[0037] (1) Callus induction culture:
[0038] In various embodiments of the present invention, hypocotyls or cotyledons are used as explants, with reference to the application number 201611144975.7, the method of the Chinese invention patent named Shami callus tissue and method for inducing proliferation thereof, to obtain Shapont callus organization (see figure 1 In A and B), the used callus medium includes: MS+0.5mg/L 6-BA+3.0mg/L 2,4-D+0.70% agar+2.0% sucrose, pH 5.8-6.0.
[0039] (2) Induction culture of adventitious buds
[0040] Inoculate the Saponica callus mass obtained in step (1) in the adventitious bud induction medium to carry out bud induction culture;
[0041] The adventitious bud induction medium used included: MS+2 mg/L 6-BA+1 mg/L NAA+0.70% agar+2.0% sucrose.
[0042] Conditions for the induction and culture of adventitious buds: humidity: 40%, time: 2-6 weeks, photoperiod: 16h light/8h dark, temperature: 28°C under light conditions, 22°C under dark conditions.
[0043] Induced adventitious buds see figure 2 A, B, and C in the middle are the adventitious buds formed after 2 weeks, 4 weeks and 6 weeks of induction, respectively.

Example Embodiment

[0044] Example 2
[0045] The method for inducing the formation of adventitious roots in Saponin callus provided by the present embodiment is as follows:
[0046] The callus of Example 1 was inoculated into the adventitious root induction medium for root induction culture; the adventitious root induction culture used included: 1/2MS+3mg/L IBA+0.70% agar+2.0% sucrose, pH 5.8-6.0;
[0047] Conditions of adventitious root induction culture: temperature: 22-28°C, humidity: 40%, time: 2-6 weeks, photoperiod: 16h light/8h dark.
[0048] Induced adventitious roots figure 2 In D, the arrows point to adventitious roots formed after 6 weeks of induction.
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