Culture medium for agriophyllum squarrosum tissue culture, adventitious bud induction method and adventitious root induction method
A technology of tissue culture and culture medium, applied in the field of culture medium of Sapona tissue culture, to achieve the effects of high rooting efficiency, improved induction efficiency and high induction rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0035] Example 1
[0036] The method for inducing Saponica callus to form adventitious buds provided by the present embodiment is as follows:
[0037] (1) Callus induction culture:
[0038] In various embodiments of the present invention, hypocotyls or cotyledons are used as explants, with reference to the application number 201611144975.7, the method of the Chinese invention patent named Shami callus tissue and method for inducing proliferation thereof, to obtain Shapont callus organization (see figure 1 In A and B), the used callus medium includes: MS+0.5mg / L 6-BA+3.0mg / L 2,4-D+0.70% agar+2.0% sucrose, pH 5.8-6.0.
[0039] (2) Induction culture of adventitious buds
[0040] Inoculate the Saponica callus mass obtained in step (1) in the adventitious bud induction medium to carry out bud induction culture;
[0041] The adventitious bud induction medium used included: MS+2 mg / L 6-BA+1 mg / L NAA+0.70% agar+2.0% sucrose.
[0042] Conditions for the induction and culture of ad...
Example Embodiment
[0044] Example 2
[0045] The method for inducing the formation of adventitious roots in Saponin callus provided by the present embodiment is as follows:
[0046] The callus of Example 1 was inoculated into the adventitious root induction medium for root induction culture; the adventitious root induction culture used included: 1 / 2MS+3mg / L IBA+0.70% agar+2.0% sucrose, pH 5.8-6.0;
[0047] Conditions of adventitious root induction culture: temperature: 22-28°C, humidity: 40%, time: 2-6 weeks, photoperiod: 16h light / 8h dark.
[0048] Induced adventitious roots figure 2 In D, the arrows point to adventitious roots formed after 6 weeks of induction.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap