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Application of MiR-205-5p in preparation of vascularization agent

An angiogenesis and capillary technology, applied in the direction of medical preparations containing active ingredients, gene therapy, organic active ingredients, etc., can solve the problem of unclear ability to regulate gastric cancer angiogenesis, and achieve the effect of inhibiting gastric cancer angiogenesis

Inactive Publication Date: 2020-12-08
PEKING UNIV FIRST HOSPITAL
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Problems solved by technology

[0003] Therefore, the signaling molecules that regulate angiogenesis have become the focus of current research. Angiogenesis itself is not an active process, it is controlled by some angiogenesis factors and some angiogenesis inhibitors, but the ability of miR-205-5p to regulate angiogenesis in gastric cancer It is still unclear, so those skilled in the art provide an application of MiR-205-5p in the preparation of angiogenesis agents to solve the problems raised in the above background technology

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  • Application of MiR-205-5p in preparation of vascularization agent

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[0021] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0022] The application of a kind of MiR-205-5p designed by the present invention in the preparation of angiogenesis agent. During the application experiment, all patients obtained written consent. A total of 109 cases of paraffin-embedded gastric cancer, normal tissue and 7 cases of liver metastases ;

[0023] Cell culture and transfection Human gastric cancer cell lines BGC823 and MGC803 were obtained from the Cancer Institute of the Chinese Academy of Medical Sciences. All c...

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Abstract

The invention relates to the technical field of blood vessel production inhibition and discloses application of MiR-205-5p in preparation of a vascularization agent. The application comprises the following operations: detecting expression of miR-205-5p and CD31 in human gastric carcinoma tissue and liver metastatic tumor by adopting quantitative real-time qRT-PCR; detecting formation of capillaryvessels of endothelial cells of human umbilical veins in vitro, and researching actions of silence and high expression of the miR-205-5p in a body dorsa body wrinkle chamber model; and detecting influence on expression of VEGFA and FGF1 caused by the miR-205-5p by adopting the qRT-PCR and Western blots, wherein the MiR-205-5p lowers the expression of the VEGFA and FGF1 through an ERK signal way and direct targeting. According to the application, the expression of the VEGFA and FGF1 is lowered through the ERK signal way and direct targeting, so that vasculogenesis of gastric carcinoma is inhibited; particularly, the MiR-205-5p exerts an important action in the vasculogenesis; and the miR-205-5p can be used for lowering the formation of newborn blood vessels through negatively regulating andcontrolling the expression of the VEGFA, so that the miR-205-5p is a carcinoma inhibiting factor through inhibiting the vasculogenesis in the gastric carcinoma, and also exerts an important action onproliferation and invasion of different types of tumors.

Description

technical field [0001] The invention relates to the technical field of angiogenesis inhibition, in particular to the application of MiR-205-5p in the preparation of angiogenesis agents. Background technique [0002] Gastric cancer (GC) is the fifth most common cancer and the third leading cause of cancer death worldwide. Almost two-thirds of gastric cancer cases occur in developing countries. With the introduction of postoperative adjuvant therapy, the prognosis of gastric cancer patients has been greatly improved. However, the median overall survival (OS) of patients with metastatic and / or unresectable gastric cancer is less than 12 months [3]. The proportion of patients benefiting from targeted therapy is still very limited. Tumor angiogenesis plays an important role in the growth, invasion and metastasis of gastric cancer, and anti-angiogenesis therapy is one of the most important and promising treatments in clinical oncology; [0003] Therefore, the signaling molecul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K31/7088A61P35/00
CPCA61K31/7088A61P35/00
Inventor 张俊岭庞晓丛王欣崔一民刘玉村
Owner PEKING UNIV FIRST HOSPITAL
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