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Construction method of non-alcoholic fatty liver mouse model

A construction method and non-alcoholic technology, applied in the field of medicine, can solve the problems of unscientific and authentic models, not green, and high cost, and achieve the effects of good stability, low cost, and high success rate

Inactive Publication Date: 2020-12-18
NORTHWEST UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The non-alcoholic fatty liver disease model constructed above is either made by using chemical drugs to damage the liver, or the selection period is very long, the cost is high, it is not green, and the pathology of the normal non-alcoholic fatty liver is quite different. The model is unscientific and authentic

Method used

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  • Construction method of non-alcoholic fatty liver mouse model
  • Construction method of non-alcoholic fatty liver mouse model
  • Construction method of non-alcoholic fatty liver mouse model

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The construction of embodiment 1 non-alcoholic fatty liver mouse model

[0038] The non-alcoholic fatty liver mouse model of the present invention is mainly constructed by using high-fat, high-sugar diet and circadian rhythm disorder to induce non-alcoholic fatty liver, so as to establish the non-alcoholic fatty liver mouse model.

[0039] Its construction method includes the following steps:

[0040] Step 1: 32 SPF grade C57BL / 6J male mice weighing 16±2 g were purchased from Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences. After one week of adaptive feeding, they were randomly divided into two groups, namely the high-fat and high-sugar diet and rhythm disorder modeling group, and the normal diet and normal work and rest control group.

[0041] Step 2: Prepare high-fat and high-sugar feed and common feed according to the formula. The model group was fed with high-fat and high-sugar feed, and the control group was fed with ordinary feed....

Embodiment 2

[0043] Example 2 Checking and judging whether the mouse model of non-alcoholic fatty liver is successfully established

[0044] The check method is as follows:

[0045] Liver gross specimen examination:

[0046] After sacrificing the mice, open the abdomen and observe the general shape, size and color of the liver with the naked eye, feel the texture of the liver with the pulp of the fingers, separate the liver and touch the cut surface of the liver. It can be seen that the liver of mice in the control group was reddish-brown, soft in texture, and there was no obvious fatty tissue near the hepatic ligament. In the model group (modeling group), some areas of the liver were light yellow, the liver was slightly enlarged, and the liver capsule was slightly tense. Slightly greasy on the cut surface.

[0047] Liver index:

[0048] At the 8th and 12th weeks of feeding, mice were randomly selected from each group, weighed on an empty stomach, and then sacrificed, and the whole live...

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Abstract

The invention discloses a construction method of a non-alcoholic fatty liver mouse model. According to the method, construction of a mouse non-alcoholic fatty liver model is induced by cooperation ofhigh-fat and high-sugar diet and circadian rhythm disorder. The method is short in consumed time, high in modeling success rate, low in cost and free of toxic and side effects caused by use of chemical reagents, and is called as a green animal model.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a method for constructing a mouse model of nonalcoholic fatty liver, in particular to a method for establishing a mouse model of nonalcoholic fatty liver induced by a high-fat, high-sugar diet and a rhythm disorder. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) refers to a pathological manifestation characterized by the accumulation of adipose tissue (≥5%) in the liver without a history of alcohol intake. In the general population, the prevalence of non-alcoholic fatty liver is more than 10%, while in developed countries, it is more than 30%. Among obese people, the prevalence of non-alcoholic fatty liver is as high as 75%. In the past ten years, with the improvement of people's living conditions and the change of lifestyle, its prevalence rate is still rising. According to research reports, the pathogenesis of non-alcoholic fatty liver is not only relate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02G01N21/84G01N1/36G01N1/30G01N1/28
CPCA01K67/02G01N1/286G01N1/30G01N1/36G01N21/84G01N2001/2873
Inventor 马忠仁陈侃马剑波程燕潘秋卫
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
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