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Chinese yam protein extract with pruritus-causing activity as well as preparation method and application thereof

A protein extract, yam technology, applied in the field of biomedicine, to achieve the effects of not easy to store, good application prospects, and high scientific research value

Inactive Publication Date: 2020-12-18
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, it is not yet clear which ingredients in yam cause human skin itching through which mechanism

Method used

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  • Chinese yam protein extract with pruritus-causing activity as well as preparation method and application thereof
  • Chinese yam protein extract with pruritus-causing activity as well as preparation method and application thereof
  • Chinese yam protein extract with pruritus-causing activity as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation method of yam protein component with pruritic activity and its pruritus evaluation.

[0027] A preparation method of yam protein extract with pruritic activity specifically comprises the following steps.

[0028] 1. Sample pretreatment.

[0029] Wash and slice the yam, freeze at -20°C for 12 hours, take 500g of yam sample and thaw it, add 1L of purified water, homogenize for 20min to obtain a solid-liquid mixture, centrifuge at 11500rpm at 4°C for 25min, take the supernatant, and measure the pH of the supernatant to be 6.35 , and the conductivity was 3.0 mS / cm, and the sample solution 1 was obtained.

[0030] Concentrate sample solution 1 with a 3kDa ultrafiltration membrane to a volume of about 200ml, adjust the pH to 8.0 with NaOH, and perform membrane filtration in the order of 0.8μm+0.45μm to obtain sample solution 2.

[0031] 2. Purification process.

[0032] Wash the Q-HPR prepacked column with 20mM Tris, pH 8.0 solution (solution A), wash...

Embodiment 2

[0037] Example 2 Component analysis of pruritic protein.

[0038] The yam protein component with itching activity prepared in Example 1 was analyzed by liquid chromatography-mass spectrometry, and the peptide was dissolved with liquid chromatography mobile phase A (aqueous solution of 0.1% formic acid and 2% acetonitrile), EASY-nLC 1000 ultra-high performance liquid phase system was used for separation. Liquid phase gradient setting: 0-90min, 6%~22%B; 90-112min, 22%~35%B; 112-116min, 35%~80%B; 116-120min, 80%B. The flow rate was set at 400 nL / min. Peptides were separated by an ultra-high performance liquid phase system and injected into an NSI ion source for ionization, and then analyzed by an Orbitrap Fusion mass spectrometer. The data acquisition mode uses the Data Dependent Scan (DDA) procedure.

[0039]Database Search MS / MS data were searched using Maxquant (v1.5.2.8). Search parameter setting: the database is Dioscorea_oppositifolia_TX (44669 sequences), the anti-base...

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Abstract

The invention belongs to the technical field of biological medicines, and relates to an pruritus-causing tool medicine, in particular to a protein extract which is separated and purified from Chineseyam and has pruritus-causing activity as well as a preparation method and application of the protein extract. The Chinese yam protein component with pruritus-causing activity is prepared by extractinga protein aqueous solution with pruritus-causing activity from Chinese yams through a water extraction method and carrying out experimental screening and elution procedures, and the Chinese yam protein component is applied to preparation of itching-pruritus tool medicines. Experiments prove that the Chinese yam protein extract has pruritus-causing activity and has wide application prospects in pruritus model tool medicine research, biological detection, disease diagnosis and treatment and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a tool medicine for causing itching, in particular to a protein extract with pruritus activity separated and purified from Chinese yam and its preparation method and application. Background technique [0002] Yam is Dioscorea opposithunb (Dioscorea L.) plant of Dioscoreaceae (Dioscoreaceae), which is a perennial winding herb. Yam is a common food in China, and it is also a medicinal material in traditional Chinese medicine prescriptions. The main components in yam are starch, polysaccharide, protein, saponin, allantoin, dopamine, flavonoids and so on. [0003] During the processing of yam, the human skin comes into contact with yam juice, and a strong itching sensation will appear at the contact site. Pruritus, an unpleasant sensation that triggers scratching, is a common clinical symptom that can occur in primary skin lesions such as dermatitis, eczema, urticaria, contact der...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/18C07K1/34C07K1/36C12N9/50A61K38/01
CPCC07K1/36C07K1/34C07K1/18C12N9/63A61K38/011
Inventor 高兴华孙冬红齐瑞群洪玉晓王忆宁王扬斌郭昊陈洪铎
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV