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Uranyl ion detection method

A technology of uranyl ion and solution is applied in the detection field of uranyl ion to achieve the effects of easy operation, improved sensitivity and reduced cost

Pending Publication Date: 2020-12-22
CHONGQING TECH & BUSINESS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] There is no report on the detection method of uranyl ion based on entropy-driven catalytic reaction and DNase strategy

Method used

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Embodiment Construction

[0026]The present invention will be further described in detail below in conjunction with the embodiments.

[0027]Such asfigure 1 As shown, the principle of the present invention is: in the presence of uranyl ions, uranyl ion-specific DNA enzymes are sheared and released DNA fragments. It can then bind to the sequence Q of the DNA complex and replace the sequence P through a competitive reaction. After sequence P leaves, a new toehold domain on sequence Q is exposed. The fuel chain can then bind to sequence Q at the newly formed toehold domain. Due to the more complementary base pairs between the fuel chain and the sequence Q, the fuel chain can replace the DNA fragments of the sequence R and DNase through a competitive reaction. The replaced DNA fragment can be combined with another DNA complex for the next competitive reaction cycle. Importantly, the process is driven by the increase in entropy, because the total number of base pairs of reactants and products before and after the re...

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Abstract

The invention provides an entropy-driven catalytic reaction-induced DNA molecule hairpin to be converted into a DNA enzyme structure, and discloses an ultra-sensitive 'opening' strategy for uranyl iondetection. In particular, uranyl ion specific DNA enzymes can be cleaved in the presence of uranyl ions and produce DNA fragments. The released DNA fragment can initiate an entropy-driven catalytic reaction to generate a large number of sequences R, the sequences R can open a cyclic structure of a DNA molecule hairpin and convert the cyclic structure into an Mg<2+> specific DNA enzyme structure,and in the presence of Mg<2+>, the DNA enzyme shearing reaction causes an FAM marker to leave the surface of gold nanoparticles to cause strong fluorescence intensity recovery. The detection limit canreach 4 pM. The strategy shows a satisfactory result in the actual application of an actual water sample. The method can be used for on-site analysis of the uranyl ion concentration in water.

Description

Technical field[0001]The invention relates to the field of detection of uranyl ions, in particular to the field of detection methods for uranyl ions based on entropy-driven catalytic reaction to induce hairpin to DNA enzyme structure conversion.Background technique[0002]Uranium has high radioactivity and genetic toxicity, which may cause serious problems to human health and environmental protection. Uranium can enter and deposit in plants and animals, and lead to the accumulation of uranium in the human body through the food chain. It has been found that even at trace levels, uranium can cause long-term and serious diseases, including kidney disease, urinary system damage and lung cancer. Uranyl ion (UO22+) Is the most stable uranium species in the human body. Due to its good bioavailability, it has a high human health risk. Therefore, it is necessary to explore a sensitive and selective method to detect UO22+Public safety and environmental protection.[0003]At present, uranyl is mai...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N31/10
CPCG01N21/6428G01N21/643G01N31/10
Inventor 云雯吴虹尤琳烽杨丽珠熊政委
Owner CHONGQING TECH & BUSINESS UNIV
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