A responsive nanoparticle with mitochondrial targeting function, its preparation method and application

A nanoparticle and responsive technology, applied in the field of nanomaterials, can solve the problems of single mitochondrial function and limited mitochondrial damage, and achieve the effects of prolonging long circulation time, increasing tumor cell uptake, and uniform morphology

Active Publication Date: 2021-12-14
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing materials that can damage mitochondria have a single function and have limited damage to mitochondria

Method used

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  • A responsive nanoparticle with mitochondrial targeting function, its preparation method and application
  • A responsive nanoparticle with mitochondrial targeting function, its preparation method and application
  • A responsive nanoparticle with mitochondrial targeting function, its preparation method and application

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preparation example Construction

[0035] The invention provides a method for preparing responsive nanoparticles with mitochondrial targeting function, comprising: S1) mixing inorganic calcium salt, dopamine and alcohol solvent to obtain a precursor solution; S2) mixing the precursor solution with carbonic acid Ammonium hydrogen is placed in a closed environment without contact with each other, and heated to react to obtain responsive nanoparticles.

[0036] Among them, the present invention has no special limitation on the types of all raw materials, as long as they are commercially available.

[0037]The inorganic calcium salt, dopamine and alcohol solvent are mixed to obtain a precursor solution; the inorganic calcium salt is preferably calcium chloride and / or calcium nitrate; the alcohol solvent is preferably ethanol; the mass ratio of the inorganic calcium salt to dopamine Preferably (10~20):1, more preferably (12~18):1, more preferably (14~16):1, most preferably 15:1; the ratio of the inorganic calcium sa...

Embodiment 1

[0053] (1) Weigh 150 mg of calcium chloride, 10 mg of dopamine, and 20 mg of curcumin into a beaker containing 100 ml of ethanol to dissolve.

[0054] (2) Weigh 10 mg of cisplatin and dissolve it in 2 ml of DMSO, and disperse the solution into the above (1) beaker A.

[0055] (3) Weigh 5 grams of ammonium bicarbonate and place it in beaker B.

[0056] (4) Place the A and B beakers in a vacuum desiccator at 40 °C for 10 h.

[0057] (5) Centrifuge at 8000 rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain responsive nanoparticles, which are designated as PDACaPtCur nanoparticles.

[0058] (6) Disperse the PDACaPtCur nanoparticles in (5) in 30 ml of ethanol, add 10 ml of 20 mg / ml PEI ethanol solution (molecular weight of polyethyleneimine 800), and stir overnight.

[0059] (7) Centrifuge at 8000 rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain polyethyleneimine-modified responsive nanoparticles, which are des...

Embodiment 2

[0077] (1) Weigh 150 mg of calcium chloride and 10 mg of dopamine into a beaker containing 100 ml of ethanol to dissolve.

[0078] (2) Weigh 5 grams of ammonium bicarbonate and place it in beaker B.

[0079] (3) Place the A and B beakers in a vacuum desiccator at 40°C for 10 h.

[0080] (4) Centrifuge at 8000 rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain responsive nanoparticles, which are designated as PDACa nanoparticles.

[0081] (5) Disperse the PDACa nanoparticles in (4) in 30 ml of ethanol, add 10 ml of 20 mg / ml PEI ethanol solution (molecular weight of polyethyleneimine 800), and stir overnight.

[0082] (6) Centrifuge at 8000 rpm for five minutes to remove the supernatant, and wash twice with ethanol to obtain polyethyleneimine-modified responsive nanoparticles, which are designated as PEICa nanoparticles.

[0083] (7) Disperse the PEICa nanoparticles in (6) in 30 ml of ethanol, add 10 ml of 20 mg / ml PEG-DMDF ethanol solution, ...

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Abstract

The invention provides a method for preparing responsive nanoparticles with mitochondrial targeting function, comprising: S1) mixing inorganic calcium salt, dopamine and alcohol solvent to obtain a precursor solution; S2) mixing the precursor solution with carbonic acid Ammonium hydrogen is placed in a closed environment without contact with each other, and heated to react to obtain responsive nanoparticles. Compared with the prior art, the responsive nanoparticles prepared by the present invention are uniform in shape and size, have high thermal stability and chemical stability, can realize a variety of different wavelength light-excited responses, and can be activated in tumor cells. Continuously produce calcium ions, increase the concentration of calcium ions in cells, and cooperate with small molecule drugs to inhibit the excretion of calcium ions by mitochondria, and cooperate with small molecule drugs to destroy mitochondrial function and promote mitochondrial damage, so as to achieve the purpose of tumor treatment. In the future great potential in cancer therapy.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a responsive nanoparticle with mitochondrial targeting function, its preparation method and application. Background technique [0002] In order to effectively treat cancer, most drugs need to act on specific organelles, so there are more and more researches on organelle-targeted drug delivery systems in recent years. [0003] Mitochondria are one of the important components in cells and have multiple functions, such as regulating apoptosis, energy conversion, tricarboxylic acid cycle, oxidative stress, storing calcium ions, etc., among which storing calcium ions is one of its important functions . More than 90% of intracellular calcium ions are stored in the intracellular calcium pools - endoplasmic reticulum and mitochondria. Mitochondrial uptake of Ca 2+ It relies on the calcium transporter on the mitochondrial membrane, which is a protein that exists on th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/02A61K47/34A61K47/10A61K33/243A61K31/12A61P35/00
CPCA61K9/5115A61K9/5146A61K33/243A61K31/12A61P35/00A61K2300/00
Inventor 丁建勋郑盼丁彬彬李杲陈学思
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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