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Pseudomonas chlororaphis subsp.aurantiaca CM-6 and application thereof

A technology of Pseudomonas citricum and CM-6, applied in the direction of application, bacteria, fungicides, etc., can solve the problem of no Pseudomonas citricum inhibiting chestnut diseases, achieve the suppression of chestnut diseases, and have a good development and application prospect , Ease of industrial production

Active Publication Date: 2020-12-29
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In summary, so far there are no reports on the use of active substances produced by Pseudomonas citrus to inhibit chestnut diseases, especially related reports on the biological control of chestnut blight and / or ink blight

Method used

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  • Pseudomonas chlororaphis subsp.aurantiaca CM-6 and application thereof
  • Pseudomonas chlororaphis subsp.aurantiaca CM-6 and application thereof
  • Pseudomonas chlororaphis subsp.aurantiaca CM-6 and application thereof

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Experimental program
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Embodiment 1

[0058] Isolation and Identification of Pseudomonas chlorophis subsp.aurantiaca CM-6 CGMCCNo.18902

[0059] 1. Isolation of Pseudomonas chlororaphis subsp.aurantiaca CM-6 CGMCCNo.18902

[0060] Soil samples from the rhizosphere of chestnut trees in Huairou Chestnut Garden were collected, and single colonies of each strain were obtained by gradient dilution plate method, preserved in test tubes and identified by molecular biology. 6 CGMCC No. 18902.

[0061] 2. Identification of Pseudomonas chlororaphis subsp.aurantiaca CM-6 CGMCCNo.18902

[0062] (1) Morphological identification

[0063] Pseudomonas chlorophis subsp.aurantiaca CM-6 CGMCCNo.18902 is rod-shaped, Gram-negative, non-spore-forming, unipolar flagella, and able to move. Colonies can be formed after being cultured on KB medium for 24 hours. The colonies can produce orange pigments, round, with raised surface, smooth, sticky, easy to pick up, and neat edges.

[0064] (2) Molecular identification

[0065] Use KB mediu...

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PUM

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Abstract

The invention discloses Pseudomonas chlororaphis subsp.aurantiaca CM-6 and application thereof. The Pseudomonas chlororaphis subsp.aurantiaca CM-6CGMCC No.18902 disclosed by the invention can obviously inhibit the growth of the mycelia of Cryphonectria parasitica and / or Phytophthora cinnamon, and an efficient inhibiting effect is achieved. The Pseudomonas chlororaphis subsp.aurantiaca CM-6CGMCC No.18902 is simple in culture condition and easy to store, industrial production is easy, and good development and application prospects are achieved.

Description

technical field [0001] The invention relates to a kind of Pseudomonas citrus and its application in the field of biotechnology. Background technique [0002] my country is a large agricultural country, and the output and quality of grain, fruits and vegetables are related to people's livelihood and social stability. The economic loss of major crops caused by plant diseases in the world is hundreds of billions of dollars, and 70%-80% of the diseases are caused by pathogenic fungi, such as the pathogenic fungus that causes chestnut blight is Chestnut blight. [0003] Chestnut blight (Cryphonectria parasitica) belongs to Ascomycota (Ascomycota), Sordariomycetes (Sordariomycetes), Diaporthales (Diaporthales) and Cryphonectriaceae (Cryphonectriaceae), and can be parasitic on European chestnut, American chestnut, chestnut, Japanese chestnut, Chestnut and other oaks. Chestnut seedlings and fruiting trees can be infected by Chestnut blight. After the onset, the diseased spots quic...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/27A01P3/00A01G13/00C12R1/38
CPCA01N63/27C12N1/20C12N1/205C12R2001/38
Inventor 程旭秦岭曹庆芹安东尼·比斯利
Owner BEIJING UNIV OF AGRI
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