Authenticity evaluation method of Tibet sophora davidii honey

An evaluation method and authenticity technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as unclear basis of substance action, changes in chemical composition structure, unstable content, etc., to achieve the effect of easy operation and promotion

Active Publication Date: 2021-01-01
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Numerous test results show that Sophora japonica uniflora honey has good antibacterial, anti-inflammatory, anti-tumor effects, and its effect is even better than Manuka honey, but the basis of the substances in Sophora japonica uniflora honey is still unclear
[0004] Although there have been some studies on the chemical constituents of Sophora syringae in the field of plant research, the formation of honey is a relatively complicated process, and it is difficult to judge which compounds can be collected by bees, migrate and enrich in the process.

Method used

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  • Authenticity evaluation method of Tibet sophora davidii honey
  • Authenticity evaluation method of Tibet sophora davidii honey
  • Authenticity evaluation method of Tibet sophora davidii honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] In this embodiment, firstly, a large amount of Sophora japonica honey and other common honeys were sampled and analyzed by the positive and negative modes of liquid chromatography tandem high-resolution mass spectrometry. The experimental results show that there is a compound with a high content in the total ion chromatogram of the honey of Sophora japonica in the positive mode, such as figure 2 As shown, the picture A is the total ion current chromatogram of the pollen of Sophora pterosaurs, and the picture B is the precise extraction ion current chromatogram of the compound found in higher content, and its chromatographic retention time is 3.33min.

[0057] Based on the accurate mass m / z 265.19105 provided by high-resolution mass spectrometry (such as image 3 Middle A figure), the present embodiment obtains the elemental composition of this material to be C 15 h 25 N 2 o + . In the MS / MS spectrum of this substance; major fragments of peaks for this substance con...

Embodiment 2

[0062] 1. Sample source

[0063] Buy common real honey samples from the market or beekeepers to detect the content of oxymatrine in various honeys, specifically including: acacia, jujube flower, sandy locust tree, rapeseed, litchi, longan, vitex, eucalyptus, snow Four samples of fat lotus, sunflower, tallow, pomegranate, wild osmanthus, wild osmanthus, caragana, manuka, old melon head, wolfberry, codonopsis, gall, citrus honey, and duck foot wood, and the sources of the four samples are different (Origin varies and / or degree of processing varies).

[0064] 2. Solution preparation

[0065] Extraction solution: pipette 20mL of methanol, and dilute to 100mL with ultrapure water. Store at 4°C.

[0066] Oxymatrine standard solution: Weigh 10 mg of oxymatrine standard solution, and dilute to 10 mL with methanol. Store at 4°C, valid for 2 months.

[0067] 3. Q Exactive plus test conditions are as follows

[0068] (1) Sample processing

[0069] Weigh 0.8g honey and add 7.2mL ex...

Embodiment 3

[0081] 1. Sample source

[0082] A total of 20 samples of various grades or brands of honey labeled as Sophora japonica honey were purchased from the market.

[0083] 2. Experimental steps

[0084] (1) Solution preparation

[0085] Extraction solution: pipette 20mL of methanol, and dilute to 100mL with ultrapure water. Store at 4°C.

[0086] Oxymatrine standard solution: Weigh 10mg of oxymatrine standard solution, dilute to 10mL with methanol, store at 4°C, valid for 2 months.

[0087] (2) Sample processing

[0088] Weigh 0.8g honey and add 7.2mL extraction solution, vortex at room temperature until completely dissolved, and centrifuge at 20194g, 4°C for 20min. The supernatant was collected and passed through a 0.22 μm filter membrane, and then 100 μL of the extract: 900 μL of methanol water was diluted once. The diluent was transferred to a sample vial for analysis on the machine.

[0089] (3) Quality control samples

[0090] Weigh 0.8g of blank honey (without oxymatr...

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Abstract

The invention belongs to the field of food detection, and particularly relates to an authenticity evaluation method of Tibet sophora davidii honey. The method comprises the following steps of using oxymatrine as a characteristic compound, when the content of oxymatrine in honey is 4-17mg/kg, judging the honey as Tibet sophora davidii honey; and when the content of the sophora japonica honey in thehoney is lower than 4mg/kg, determining that the honey is other honey or adulterated Tibet sophora davidii honey. The method provided by the invention has the advantages of simplicity, high efficiency and the like, is convenient to operate and popularize, has a certain guiding significance for formulation of related industries or national standards of high-quality Tibet sophora davidii honey, andalso has an important practical significance for protecting legitimate rights and interests of honey consumers and maintaining healthy development of the honey consumption industry.

Description

technical field [0001] The invention relates to the field of food testing, in particular to a method for evaluating the authenticity of Sophora japonica honey. Background technique [0002] Nectar plants are the basis for bees to obtain food nutrition and produce honey. At present, there are thousands of nectar plants known, among which the nectar plants that are rich in honey and can be produced by bees in large quantities are usually called the main nectar plants. At present, the types of main nectar source plants in my country are very limited, only more than 40 species, such as acacia, linden, Vitex, jujube, litchi, longan, sunflower, tallow, duck foot wood, gallnut, etc. Under normal circumstances, most nectar plants have limited nectar production, which can only basically maintain the survival and reproduction of bee colonies and produce a small amount of nectar. These nectar plants are called auxiliary nectar plants, and there are thousands of species. It is worth n...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884Y02A50/30
Inventor 杨术鹏李熠张玉豪周金慧张金震杨宇晖
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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