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Application of a strain of Lactobacillus plantarum in the preparation of a composition for alleviating chronic inflammation in the body

A technology of Lactobacillus plantarum and chronic inflammation, which is applied in the field of microorganisms, can solve the problems of limited chronic inflammation, differences in the effect of different strains, and obvious differences in individual intestinal flora and physiological state, so as to relieve chronic inflammation, prevent diseases, and achieve good health. The effect of stability

Active Publication Date: 2022-04-26
HANGZHOU WAHAHA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these strains are dose- and strain-specific, and individual intestinal flora and physiological state are significantly different, and the effects of different strains are different. Therefore, it is still necessary to develop other new probiotic strains that can relieve chronic inflammation
In addition, the types of inflammatory factors that can be regulated by the existing Lactobacillus plantarum are limited. However, in the chronic inflammation caused by different reasons, the types of inflammatory factors that change are different, and different inflammatory factors have different effects on the body. Therefore, it is now Some Lactobacillus plantarum can adapt to limited chronic inflammation
[0004] In the previous research, the applicant isolated a new strain of Lactobacillus plantarum (Lactobacillus plantarum) 1701 from yogurt powder samples collected in Tibet, and conducted research on its application in weight loss, but whether the strain has the effect of alleviating The ability of chronic inflammation and whether it can have more physiological activities need to be further studied

Method used

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  • Application of a strain of Lactobacillus plantarum in the preparation of a composition for alleviating chronic inflammation in the body
  • Application of a strain of Lactobacillus plantarum in the preparation of a composition for alleviating chronic inflammation in the body

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Experimental program
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Embodiment 1

[0035] The strain provided by the present invention has been identified as belonging to Lactobacillus plantarum, named as 1701 strain, and was preserved in the General Microorganism Culture Collection Center of China Microorganism Culture Collection Management Committee on October 23, 2019, and the microorganism preservation number is CGMCC NO.18728.

[0036] The strain provided by the present invention is isolated by the inventor from a yogurt powder sample collected from the countryside of Shigatse City, Tibet Autonomous Region of my country.

[0037] The 16S rRNA gene sequence of the strain Lactobacillus plantarum 1701 of the present invention is shown in SEQ ID NO: 1.

Embodiment 2

[0039] Kunming mice were reared at a temperature of 21±2°C, a humidity of 30-70%, 12 hours of alternating light, and free access to mouse maintenance feed and drinking water. After being reared for 1 week in Kunming, the mice were sacrificed by removing their cervical vertebrae, and the spleen of the mice was taken aseptically, and crushed with a sterile glass syringe core. The lysis was terminated with sterile Hank's solution of % fetal bovine serum, centrifuged at 1000 rpm for 5 min at 4° C., and the pellet was resuspended in 5 mL of RPMI-1640 medium containing 10% fetal bovine serum. The cells were stained with trypan blue and counted on a hemocytometer to calculate the number of viable cells and the percentage of viable cells. Adjust the cell concentration to 5 × 10 6 cells / mL.

[0040]After the second-generation activation of the strain Lactobacillus plantarum 1701 of the present invention and the reference commercial strain Lactobacillus rhamnosus GG (LGG), the bacter...

Embodiment 3

[0046] The spleen lymphocyte suspension was prepared according to the preparation method of mouse spleen lymphocytes in Example 2, and the cell concentration was adjusted to 5×10 6 cells / mL.

[0047] After the second-generation activation of the strain Lactobacillus plantarum 1701 of the present invention and the reference commercial strain Lactobacillus rhamnosus GG (LGG), the bacterial concentration was adjusted to 1×10 7 CFU / mL.

[0048] The cell suspension was added to a 96-well cell culture plate, with 5 replicates per treatment, and divided into zero adjustment group (cell culture medium), blank control group (cell culture medium + cell suspension), and bacterial treatment group (cell culture medium + Cell suspension + bacterial suspension 1×10 7 CFU / mL), 37°C 5% CO 2 Incubate for 48 h in the incubator. After the incubation, centrifuge at 1500 rpm for 10 min, aspirate the supernatant, filter with a 0.22 μm filter, and measure the IL-10 and IL-12 content by ELISA ki...

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Abstract

The invention relates to the field of microorganisms, and discloses the application of a plant lactobacillus strain in the preparation of a composition for relieving chronic inflammation in a body. The Lactobacillus plantarum was named 1701, and it was preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms on October 23, 2019. Its preservation number is CGMCC No.18728, and the microbial classification is named Lactobacillus plantarum Lactobacillus plantarum . The Lactobacillus plantarum used in the present invention can simultaneously regulate the levels of cytokine IL-12, anti-inflammatory factor IL-10 and pro-inflammatory factors (including IL-1β, IL-6, TNF-α, MCP-1 and NO), Therefore, it can adapt to chronic inflammation caused by different reasons, and can more comprehensively prevent diseases caused by chronic inflammation.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the application of a Lactobacillus plantarum in the preparation of a composition for relieving chronic inflammation of the body. Background technique [0002] In recent years, with the continuous improvement of people's living standards, a series of health problems have emerged, and sub-health problems have become increasingly prominent. The main reasons for sub-health are: unreasonable diet, irregular work and rest, lack of exercise, lack of sleep, psychological pressure, mental stress, long-term negative emotions, etc. Studies have found that different sub-healthy patients are more or less accompanied by the occurrence of chronic inflammation in the body, and accompanied by the production of MCP-1, which promotes macrophage infiltration and subsequent secretion of inflammatory factors (such as IL-10, IL-6 and IL-6). , TNF-α and NO, etc.). Once macrophages are activated, the acti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/747A61P29/00C12N1/20A23L33/135A23L2/38A23L2/385A23C19/00A23C9/123A23C9/16A23L19/00A23L29/00C12R1/25
CPCA61K35/747A61K9/0053A61P29/00A23L33/135A23L2/382A23L2/385A23L19/00A23C9/1234A23C9/16A23C19/00A23L29/065A23V2002/00A23V2400/169A23V2200/30
Inventor 孙盛俞赟霞陈苏李言郡余腾斐陈作国陈丽娥郑志瑶陈彩玲周晴晴任学良
Owner HANGZHOU WAHAHA TECH