Ad7 vector vaccine for preventing SARS-CoV-2 infection

A vector vaccine, sars-cov-2 technology, applied in the field of Ad7 vector vaccine, can solve the problems of low expression level, insufficient resistance to virus infection, invalid vaccine titer, etc., and achieve the effect of convenient use and good safety

Active Publication Date: 2021-01-12
GUANGZHOU N BIOMED LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, experiments have shown that the natural spike protein S gene of SARS-CoV-2 is expressed at a very low level in human kidney cells HEK2...

Method used

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  • Ad7 vector vaccine for preventing SARS-CoV-2 infection
  • Ad7 vector vaccine for preventing SARS-CoV-2 infection
  • Ad7 vector vaccine for preventing SARS-CoV-2 infection

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Experimental program
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Embodiment Construction

[0033] The amino acid sequence of the Spike protein (S) of SARS-CoV-2 is shown in YP_009724390.1, which is denoted as NB1.

[0034]The pre-mRNA transcribed by eukaryotic cells can produce different mRNA splicing isoforms through different splicing methods (selecting different combinations of splicing sites), which ultimately leads to different proteins produced by the same gene sequence. This is very detrimental to protein expression. The inventor optimized the codons of the wild-type natural nucleic acid sequence and removed potential variable splicing sites based on his own technology, which ensured the uniqueness of protein expression and reduced the difficulty of subsequent protein purification. 优化得到的核酸序列记为NB2,其具体序列如SEQ ID NO:1所示: ATGTTCGTGTTTCTGGTGCTGCTGCCTCTGGTGAGCTCCCAGTGCGTGAACCTG ACCACAAGGACCCAGCTGCCACCTGCCTATACCAATAGCTTCACACGGGGCGT GTACTATCCCGACAAGGTGTTTAGATCTAGCGTGCTGCACTCCACCCAGGATCT GTTTCTGCCTTTCTTTTCTAACGTGACATGGTTCCACGCCATCCACGTGTCCGGC ACCAATGGCACAAAGCGGTTCGACAAT...

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Abstract

The invention discloses an Ad7 vector vaccine and an adenovirus vector vaccine for preventing SARS-CoV-2 infection. The adenovirus vector vaccine comprises an Ad7 vector loaded with a nucleic acid sequence shown in SEQ ID NO: 1. In some embodiments, the adenovirus vector vaccine has better safety and are convenient to use. Experiments show that more S proteins can be generated in human cells. Theadenovirus vector vaccine is expected to be developed into the Ad7 vector vaccine for preventing SARS-CoV-2 infection. In some embodiments, the adenovirus vector vaccine can be used in combination with other vaccines, can also be used as a therapeutic vaccine for new coronal pneumonia, and can be inoculated at the initial stage of infection of a patient to quickly induce immune response in a humanbody so as to achieve a therapeutic effect.

Description

technical field [0001] The present invention relates to an Ad7 vector vaccine for preventing SARS-CoV-2 infection. Background technique [0002] Vaccines are the most cost-effective interventions to prevent and control 2019-nCoV infection. Published comparisons of more than 100 SARS-CoV-2 virus genomes show that the overall degree of mutation of the virus is low, and no recombination has occurred. Therefore, if the SARS-CoV-2 vaccine is successfully developed, it will be able to protect the population from the new coronavirus infection, thereby suppressing the outbreak of new outbreaks. [0003] In the virus particle structure of SARS-CoV-2 coronavirus, the S-protein that makes up the "crown" is an obvious target, and has become the focus of most research teams. A research team has successfully revealed the relationship between the S protein and its receptor ACE2 in the process of invading cells through computer simulation of the three-dimensional structure of the S protei...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61P31/14A61P11/00
CPCA61K39/12A61K2039/5256A61P11/00A61P31/14C12N15/86C12N2710/10343C12N2770/20034C12N2800/107
Inventor 陈凌关素华杨臣臣汪乾
Owner GUANGZHOU N BIOMED LTD
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