A method for detecting nanoscale plastic particles in animal biological samples

Abstract

The invention discloses a method for detecting nanoscale plastic particles in animal biological samples, which relates to the fields of biological monitoring using animal biological samples and toxicology research of tissues and organs. Collect biological samples such as animal seafood and rodent experimental animal organs, release the plastic particles in the samples through alkali solution digestion, use the properties of the digestion products to modify the surface of nano-plastics, and then use solid-phase adsorbents to absorb and wrap the mixed Nanoplastics were extracted by coagulation and precipitation; finally, the concentration of plastic-specific indicator compounds was detected by the Pyr-GC-MS coupled platform, and the mass concentration of the indicator compounds was converted into the mass of nanoplastics. It solves the problems caused by the different pore size of the filter membrane and the clogging of the filter membrane when extracting the plastic in the tissue sample by conventional membrane filtration. To provide a trace and ultra-trace pollution analysis method for the health problems caused by nanoplastics and the risk of population exposure. The method is simple, low in cost, high in benefit and wide in application.

Description

technical field [0001] The invention relates to the field of biological monitoring and toxicology of tissues and organs, in particular to a detection method for nanoscale plastic particles in animal biological samples. Background technique [0002] Since the development of the plastic field in the middle of the 20th century, the world's plastic production has grown exponentially. Plastic products are widely used in various fields due to their advantages of low price and durability, especially the large increase in the use of disposable plastic products, which directly leads to Plastic waste is increasing year by year. Due to poor management of plastic waste, the ocean has become the final destination of most plastic waste. The large amount of plastic waste in the marine environment directly affects the survival and growth of marine life. In addition to large particles of plastic waste, nanoplastics that are produced through physical and chemical actions and can enter the b...

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Problems solved by technology

[0004] Despite the rapid development of plastic research in the past five years, and some studies have attempted to study the exposure of plastic particles from the level of biomonitoring, the detection technology is limited to characterizing micron-sized plastics, and the extraction and quantification methods used in various studies are different. First, the lack of comparability among various results ([9] Li P, et al. Analytical methods and environmental processes of nanoplastics. Environ. Sci. 2020; 94:88-99.), leading to poor promotion of various detection techniques, And there is a lack of qualitative and quantitative methods for nanoplastic particles in tissue samples

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