VCAM-1 monoclonal antibody tanshinone IIA nanostructure lipid carrier as well as preparation method and application thereof
A technology of VCAM-1 and nano-structured lipids, which is applied in the research field of new drug delivery systems, can solve the problems of increasing the incidence of adverse drug reactions, affecting the effective treatment of diseases, and low distribution, so as to improve the efficiency of drug treatment and relieve kidney pain. Inflammation level, effect of reducing distribution
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0031] On the other hand, the present invention provides a method for preparing VCAM-1 monoclonal antibody Tanshinone IIA lipid nanoparticles, the steps are as follows:
[0032] 1) Accurately weigh 10% Tanshinone IIA, 84%-89% glyceryl monostearate, 0%-5% polyethylene glycol monostearate (molecular weight: 2000) and 0.5% Monoamino-terminated polyethylene glycol stearate, a total of 99.5 mg of the above materials, mixed to obtain material A;
[0033] 2) Add 0.5mL of ethanol to material A, and heat it in a water bath at 60°C to completely dissolve it to obtain material B;
[0034] 3) Using deionized water as the dispersion medium, inject 0.5mL of material B into 9.5mL of deionized water heated in a 60°C water bath at a stirring speed of 400rpm, take it out after the injection is completed and stir for 30s, and naturally cool to room temperature to obtain tanshinone IIA lipid Bulk nano dispersion;
[0035] 4) Add N,N'-disuccinimidyl carbonate to the tanshinone IIA liposome nanop...
Embodiment 1
[0039] Example 1: Preparation of VCAM-1 monoclonal antibody Tanshinone IIA nanostructure lipid carrier: Accurately weigh 10 mg Tanshinone IIA, 84 mg glyceryl monostearate, 5 mg polyethylene glycol monostearate, 0.5 mg monoamino terminal Polyethylene glycol stearate, 99.5mg in total, was dissolved in 0.5mL ethanol as the organic phase, heated in a water bath at 60°C to completely melt the material, and deionized water was used as the dispersion medium, and the organic phase was sequentially mixed at a stirring speed of 400rpm Quickly inject 9.5 mL of deionized water heated in a water bath at 60°C, take out after injection and stir for 30 s, and naturally cool to room temperature to prepare tanshinone IIA lipid nanoparticles. Add 0.1mg of N,N'-disuccinimidyl carbonate as catalyst, vortex for 1min to disperse evenly, incubate at room temperature for 3h, add 100μL VCAM-1 monoclonal antibody (5mg / mL), vortex for 1min to disperse evenly Continue to incubate the reaction at room temp...
Embodiment 2
[0045] Example 2: Preparation of VCAM-1 monoclonal antibody Tanshinone IIA lipid nanoparticles, accurately weighed 10mg Tanshinone IIA, 85mg glyceryl monostearate, 4mg polyethylene glycol monostearate, 0.5mg monoamino-terminated Polyethylene glycol stearate, 99.5mg in total, was dissolved in 0.5mL of ethanol as the organic phase, heated in a water bath at 60°C to completely melt the material, and deionized water was used as the dispersion medium, and the organic phase was rapidly mixed at a stirring speed of 400rpm. Inject into 9.5 mL of deionized water heated in a water bath at 60°C, take out after injection and stir for 30 s, and cool naturally to room temperature to prepare tanshinone IIA lipid nanoparticles. Add 0.1mg of N,N'-disuccinimidyl carbonate as catalyst, vortex for 1min to disperse evenly, incubate at room temperature for 3h, add 100μLVCAM-1 monoclonal antibody (5mg / mL), vortex for 1min to disperse evenly Continue to incubate the reaction at room temperature for 3...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
| particle diameter | aaaaa | aaaaa |
| particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More