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Application of obeticholic acid to prevention and treatment of sepsis-induced myocardial injury

A technology for obeticholic acid and myocardial injury, which can be applied in the field of biomedicine and can solve problems such as high fatality rate

Inactive Publication Date: 2021-01-22
SHANGHAI CHILDRENS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although breakthroughs have been made in the treatment of septic myocardial injury in recent years, the current treatment method still has a high mortality rate, so there is an urgent need to develop clinical drugs for the treatment of sepsis and septic myocardial injury

Method used

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  • Application of obeticholic acid to prevention and treatment of sepsis-induced myocardial injury
  • Application of obeticholic acid to prevention and treatment of sepsis-induced myocardial injury
  • Application of obeticholic acid to prevention and treatment of sepsis-induced myocardial injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Obeticholic Acid Alleviates the Reduced Heart Rate of Mice Caused by Lipopolysaccharide

[0057] 1. Experimental materials:

[0058] The C57BL / 6 mice used in the present invention were purchased from Shanghai South Model Animal Center, the obeticholic acid used in the present invention was purchased from Intercept Pharmaceuticals, and the lipopolysaccharide was purchased from Sigma-Aldrich.

[0059] 2. Experimental method:

[0060] Twenty-four clean-grade male C57BL / 6 mice (8 weeks old, body weight 20-25g) were fed in the animal room, and were randomly divided into control group (n=6) and obeticholic acid group (n=6) after adaptive feeding for one week. 6), lipopolysaccharide group (n=6) and obeticholic acid+lipopolysaccharide group (n=6).

[0061] The mice in the obeticholic acid group and obeticholic acid+lipopolysaccharide group were continuously administered with obeticholic acid for 6 days (5mg / kg / day), and after 6 days, the mice in the lipopolysacchari...

Embodiment 2

[0065] Example 2 Obeticholic acid can alleviate abnormal biochemical indicators of myocardial injury caused by lipopolysaccharide in mice

[0066] 1. Experimental materials:

[0067] The C57BL / 6 mice used in the present invention were purchased from Shanghai South Model Animal Center, the obeticholic acid used in the present invention was purchased from Intercept Pharmaceuticals, and the lipopolysaccharide was purchased from Sigma-Aldrich.

[0068] 2. Experimental method:

[0069] Twenty-four clean-grade male C57BL / 6 mice (8 weeks old, body weight 20-25g) were fed in the animal room, and were randomly divided into control group (n=6) and obeticholic acid group (n=6) after adaptive feeding for one week. 6), lipopolysaccharide group (n=6) and obeticholic acid+lipopolysaccharide group (n=6).

[0070] Obeticholic acid group and obeticholic acid + lipopolysaccharide group mice were continuously administered with obeticholic acid (5 mg / kg / day), and 6 days later, mice in lipopolysa...

Embodiment 3

[0074] Example 3 Obeticholic acid can improve the pathological damage of myocardial tissue in mice caused by lipopolysaccharide

[0075] 1. Experimental materials:

[0076] The C57BL / 6 mice used in the present invention were purchased from Shanghai South Model Animal Center, the obeticholic acid used in the present invention was purchased from Intercept Pharmaceuticals, and the lipopolysaccharide was purchased from Sigma-Aldrich.

[0077] 2. Experimental method:

[0078] Twenty-four clean-grade male C57BL / 6 mice (8 weeks old, body weight 20-25g) were fed in the animal room, and were randomly divided into control group (n=6) and obeticholic acid group (n=6) after adaptive feeding for one week. 6), lipopolysaccharide group (n=6) and obeticholic acid+lipopolysaccharide group (n=6).

[0079] Obeticholic acid group and obeticholic acid + lipopolysaccharide group mice were continuously administered with obeticholic acid (5 mg / kg / day), and 6 days later, mice in lipopolysaccharide g...

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Abstract

The invention relates to the field of biomedicine, in particular to an application of obeticholic acid to prevention and treatment of sepsis-induced myocardial damage. Serological detection, RT-PCR, Western-Blot, HE staining and the like prove that the OCA can effectively relieve inflammatory response and myocardial damage degree of a sepsis mouse, improve the survival rate of the mouse and play arole in treating sepsis for the first time, so that the invention provides the application of the OCA to medicines for treating sepsis and sepsis myocardial injury.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the use of obeticholic acid in preventing and treating sepsis-induced myocardial injury. Background technique [0002] Sepsis is the main cause of death in critically ill patients. The incidence of sepsis is on the rise, and the mortality rate is as high as 34.6%. It is an important challenge for emergency and critical care medicine. Sepsis 3.0 defines sepsis as life-threatening organ dysfunction caused by an uncontrolled inflammatory response of the body to infection. Severe sepsis is often accompanied by cardiovascular dysfunction, which can lead to septic shock and multiple organ dysfunction syndrome (MODS) as the disease progresses, and myocardial damage exists in the early stage of sepsis, including myocardial systolic and diastolic dysfunction, Decreased ejection fraction, changes in the ultrastructure of cardiomyocytes. [0003] The pathogenesis of myocardial injury in sepsis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/575A61P9/00
CPCA61K31/575A61P9/00
Inventor 张育才王春霞缪惠洁唐晓梦
Owner SHANGHAI CHILDRENS HOSPITAL