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Hepatopancreatic parvovirus and nucleocapsid protein affinity screening method and application thereof

A nucleocapsid protein, screening method technology, applied in the field of hepatopancreatic parvovirus and nucleocapsid protein affinity screening

Active Publication Date: 2021-01-22
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Research on invertebrates is still in its infancy

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  • Hepatopancreatic parvovirus and nucleocapsid protein affinity screening method and application thereof
  • Hepatopancreatic parvovirus and nucleocapsid protein affinity screening method and application thereof
  • Hepatopancreatic parvovirus and nucleocapsid protein affinity screening method and application thereof

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Embodiment

[0055] The study of virus assembly mechanism is of great significance to the prevention and treatment of viral diseases. At present, the interaction and assembly mechanism between shrimp parvovirus nucleic acid and capsid protein is still unclear. Revealing the recognition mechanism of single-stranded nucleic acid and capsid protein lays the foundation for in-depth discussion and research on the use of virus-like particles as a substrate to effectively package anti-interference RNA for virus control . In recent years, we have carried out the determination and analysis of the whole gene sequence of HPV Chinese strain and tried to express the recombinant capsid protein of HPV. On the basis of the previous work, a random ssDNA library of the whole gene of shrimp hepatopancreatic parvovirus (HPV) was constructed, and the HPV nucleocapsid protein was purified by affinity chromatography and refolded. A preliminary study on the effect of HPV self-installed virus-like particles was c...

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Abstract

The invention provides a hepatopancreatic parvovirus and nucleocapsid protein affinity screening method and application thereof, and belongs to the technical field of interaction between nucleic acidand virus nucleocapsid protein. According to the invention, a nucleic acid fragment having affinity with the HPV nucleocapsid protein is successfully screened through systematic evolution of ligands by exponential enrichment (SELEX). The technical method lays a foundation for protein and nucleic acid immunoassay, provides a scientific basis for research on HPV molecular epidemiology, pathogenesisand the like, and therefore, has favorable practical application value.

Description

technical field [0001] The invention belongs to the technical field of interaction between nucleic acid and viral nucleocapsid protein, and in particular relates to an affinity screening method for hepatopancreatic parvovirus and nucleocapsid protein and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The study of virus assembly mechanism is of great significance to the prevention and treatment of viral diseases. Researchers use the characteristics of packaging sequences that can specifically recognize packaging enzymes or capsid structural proteins. More in-depth studies have been carried out, such as the interaction between the AC-rich region and...

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Application Information

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IPC IPC(8): C12N15/10C12N15/11G01N33/569G01N33/53
CPCC12N15/1048C12N15/11G01N33/56983G01N33/5308G01N2333/015Y02A50/30
Inventor 杨冰黄倢刘天奇吕秀旺李晨万晓媛王秀华张庆利宋晓玲刘莉许华
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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