SNP molecular marker related to wheat ear germination resistance and application thereof

A molecular marker and resistance-related technology, applied in the field of wheat genetics and breeding, can solve the problems of PHS disasters, halved yields, heavy losses, etc., and achieve the effects of improving targeting and pertinence, simple detection methods, and obvious differences

Active Publication Date: 2021-01-26
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

In 2016, most of the winter wheat region in the north, most of southern China in the south of the Yangtze River, parts of the eastern part of Southwest China, Jianghuai, western Huanghuai and Northwest China had frequent precipitation during the wheat harvest season, which greatly affected wheat harvesting and drying,

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  • SNP molecular marker related to wheat ear germination resistance and application thereof
  • SNP molecular marker related to wheat ear germination resistance and application thereof
  • SNP molecular marker related to wheat ear germination resistance and application thereof

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Experimental program
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Embodiment 1

[0025] Identification of Candidate Genes

[0026] In the present invention, a recombinant inbred line population (JH-RIL) containing 174 families was constructed using the PHS-susceptible promotion variety "Jing 411" and the highly PHS-resistant "Hongmangchun 21" as parents, and a simplified genome sequencing method was used to construct Based on the high-density genetic linkage map of SLAF markers, combined with the germination index (Germination index, GI) data of 5 days and 15 days after harvest measured in 2014-2019, the main effect QTL related to PHS was analyzed, and a control gene was found on chromosome 4B of wheat The major effect site of Phs, Qphs.ahau-4B. A secondary isolation population was constructed for this site for recombinant screening, and the physical segment was narrowed from 2.25Mb to 154.7Kb. Combined with transcriptome separation cluster pool pool sequencing, the only differentially expressed candidate gene encoding E3 ubiquitin ligase in this segment ...

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Abstract

The invention provides an SNP molecular marker related to wheat ear germination resistance and application thereof, and particularly relates to the technical field of genetic breeding. An SNP locus corresponds to C/T base mutation at a wheat 4B chromosome 661498776 bp of a reference genome IWGSC RefSeq v1.0 version; and a CAPS marker is developed according to the SNP molecular marker, the CAPS marker is named as TaE3, and the TaE3 has a nucleotide sequence shown as SEQ ID NO. 5 or a nucleotide sequence shown as SEQ ID NO. 6. The CAPS marker is used for identifying wheat ear germination resistant varieties. The detection method is simple and convenient, and is used for wheat anti-PHS molecular marker assisted breeding.

Description

technical field [0001] The invention belongs to the technical field of wheat genetics and breeding, and in particular relates to a SNP molecular marker related to wheat ear germination resistance and an application thereof. [0002] technical background [0003] Pre-harvest sprouting (PHS) refers to the phenomenon that wheat grains germinate directly on the ears of wheat when it encounters continuous rainy weather or long-term high-humidity environment before harvesting. The occurrence of PHS not only reduces the yield of wheat, but also easily leads to a decline in quality, and in severe cases, it even loses the value of planting, causing huge economic losses to agricultural production. For example, the storage substances (protein, starch, etc.) in the endosperm of germinated wheat are degraded, and the nutritional quality decreases accordingly; especially, the bread made of germinated wheat has poor elasticity and is not easy to be cut, and the color of steamed bread is dar...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2521/313C12Q2565/125
Inventor 曹佳佳常成张海萍卢杰马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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