SNP molecular marker related to wheat ear germination resistance and application thereof

Abstract

The invention provides an SNP molecular marker related to wheat ear germination resistance and application thereof, and particularly relates to the technical field of genetic breeding. An SNP locus corresponds to C / T base mutation at a wheat 4B chromosome 661498776 bp of a reference genome IWGSC RefSeq v1.0 version; and a CAPS marker is developed according to the SNP molecular marker, the CAPS marker is named as TaE3, and the TaE3 has a nucleotide sequence shown as SEQ ID NO. 5 or a nucleotide sequence shown as SEQ ID NO. 6. The CAPS marker is used for identifying wheat ear germination resistant varieties. The detection method is simple and convenient, and is used for wheat anti-PHS molecular marker assisted breeding.

Description

technical field [0001] The invention belongs to the technical field of wheat genetics and breeding, and in particular relates to a SNP molecular marker related to wheat ear germination resistance and an application thereof. [0002] technical background [0003] Pre-harvest sprouting (PHS) refers to the phenomenon that wheat grains germinate directly on the ears of wheat when it encounters continuous rainy weather or long-term high-humidity environment before harvesting. The occurrence of PHS not only reduces the yield of wheat, but also easily leads to a decline in quality, and in severe cases, it even loses the value of planting, causing huge economic losses to agricultural production. For example, the storage substances (protein, starch, etc.) in the endosperm of germinated wheat are degraded, and the nutritional quality decreases accordingly; especially, the bread made of germinated wheat has poor elasticity and is not easy to be cut, and the color of steamed bread is dar...

AI Technical Summary

Problems solved by technology

In 2016, most of the winter wheat region in the north, most of southern China in the south of the Yangtze River, parts of the eastern part of Southwest China, Jianghuai, western Huanghuai and Northwest China had frequent precipitation during the wheat harvest season, which greatly affected wheat harvesting and drying, resulting in serious PHS disasters. Yields almost halved, quality deteriorated, and farmers and smallholders without dryers lost even more

Although good progress has been made in the genetic mechanism of wheat ear germination resistance, its detailed genetic basis and molecular regulation mechanism are still unclear.

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