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A kind of SNP molecular marker related to wheat ear germination resistance and its application

A technology related to molecular markers and resistance, applied in the field of wheat genetics and breeding, can solve the problems of PHS disasters, halved yields, heavy losses, etc., and achieve the effects of improving targeting and pertinence, simple detection methods, and improving efficiency

Active Publication Date: 2022-03-11
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2016, most of the winter wheat region in the north, most of southern China in the south of the Yangtze River, parts of the eastern part of Southwest China, Jianghuai, western Huanghuai and Northwest China had frequent precipitation during the wheat harvest season, which greatly affected wheat harvesting and drying, resulting in serious PHS disasters. Yields almost halved, quality deteriorated, and farmers and smallholders without dryers lost even more
Although good progress has been made in the genetic mechanism of wheat ear germination resistance, its detailed genetic basis and molecular regulation mechanism are still unclear.

Method used

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  • A kind of SNP molecular marker related to wheat ear germination resistance and its application
  • A kind of SNP molecular marker related to wheat ear germination resistance and its application
  • A kind of SNP molecular marker related to wheat ear germination resistance and its application

Examples

Experimental program
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Embodiment 1

[0025] Identification of candidate genes

[0026] The invention uses the popularized variety "Jing 411" susceptible to PHS and "Hongmangchun 21" with high resistance to PHS as the parents to construct a recombinant inbred line population (JH-RIL) including 174 families, and adopts a simplified genome sequencing method to construct a recombinant inbred line group (JH-RIL) Based on the high-density genetic linkage map of SLAF markers, combined with the germination index (GI) data at 5 days and 15 days after harvest from 2014 to 2019, PHS-related major QTL analysis was performed, and a control was found on chromosome 4B of wheat. The major site of PHS Qphs.ahau-4B. A secondary segregating population was constructed against this site for recombinant screening, reducing the physical segment from 2.25Mb to 154.7Kb. The only differentially expressed candidate gene encoding E3 ubiquitin ligase in this segment was screened by combining transcriptome isolation and cluster pooling seque...

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Abstract

The invention provides a SNP molecular marker related to wheat ear germination resistance and its application, and specifically relates to the technical field of genetic breeding. The SNP site corresponds to the C / T base mutation; develop CAPS mark according to SNP molecular mark, and described CAPS mark is named as TaE3, and described TaE3 has the nucleotide sequence as shown in SEQ ID NO.5 or the nucleus as shown in SEQ ID NO.6 nucleotide sequence. CAPS markers are used to identify wheat cultivars resistant to panicle germination. The detection method of the invention is simple and convenient, and can be used for molecular marker assisted breeding of wheat resistance to PHS.

Description

technical field [0001] The invention belongs to the technical field of wheat genetics and breeding, and in particular relates to a SNP molecular marker related to wheat ear germination resistance and its application. [0002] technical background [0003] Wheat ear germination (Pre-harvest Sprouting, PHS) refers to the phenomenon that the wheat grains germinate directly on the wheat ear when it encounters continuous rainy weather before harvesting or is in a high humidity environment for a long time. The occurrence of PHS not only reduces the yield of wheat, but also easily leads to the decline of quality, and even the loss of plant value in severe cases, causing huge economic losses to agricultural production. For example, the storage substances (protein, starch, etc.) in the endosperm of germinated wheat are degraded, and the nutritional quality is degraded; especially, the bread made from germinated wheat has poor elasticity and is not easy to be cut, the color of the stea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2521/313C12Q2565/125
Inventor 曹佳佳常成张海萍卢杰马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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