F3'H allele capable of influencing purple character of radish and identification method of F3'H allele

Abstract

The invention relates to an RsF3'H allele influencing red / purple characters of radish and an identification method thereof, which is characterized in that a corresponding genetic map is constructed based on an F2 generation genetic group generated by hybridization of a high generation selfing line RR1 (red skin and red pulp) from a specific germplasm resource of Yunnan carrot and a high generation selfing line WR1 (white skin and white pulp) of processing type white radish; then according to the anthocyanin content data of plants (root barks and root flesh) of F2 generation individuals, the RsF3 'H (LOC108814778) gene is positioned to be a key gene for determining that the radish plant has a purple character potential or a red character potential; compared with a reference sequence, the haplotype RsF3'H-RR1 of the gene in the red-pulp radish has the advantages that a section of 507bp basic group is inserted at the 775bp position of the haplotype RsF3'H-RR1, which causes the RsF3'H-RR1 to form a termination codon in advance and lose the function, so that the radish presents a red character.

Description

technical field [0001] The invention relates to the field of gene research affecting the purple trait of radish, in particular to an F3 / H allele affecting the purple trait of radish and an identification method thereof. Background technique [0002] Radish (Raphanus sativus L.) is a biennial or annual horticultural crop belonging to the Brassicaceae Radish family. The perennial cultivation area in China is second only to Chinese cabbage. It occupies an important position in agricultural production and has high edible and medicinal value. Our country is rich in radish germplasm resources. Under long-term natural and artificial selection, radish varieties with different skin and flesh colors have been formed. Some radish varieties are rich in anthocyanidin, which is a high-quality natural colorant because of its stable color and antioxidant activity, and is widely used in the food and cosmetic industries. Yunnan carrot is a kind of special anthocyanin-rich germplasm resources...

AI Technical Summary

Problems solved by technology

[0011] In the process of breeding and variety improvement for Yunnan carrot quality, yield, etc., it is necessary to use a large number of parental materials for hybridization, but whether the parental materials (especially materials without anthocyanin deposition) cause the offspring materials to produce purple or red? The status of the trait is difficult to determine from phenotypic observations

Disadvantages of existing molecular markers for identifying red fleshy roots of radish: 1. Existing molecular markers do not have wide applicability, and the retrotransposon insertion in the first exon of RsF3'H is not used in Yunnan red radish materials. Not detected; 2. The functional gene located is different from the gene of this study (see related patent "a specific molecular marker for identifying the purple skin character of radish fleshy root"), because the genetic mechanism of radish anthocyanin production is different There are genotype differences in radish germplasm, so the existing markers cannot be used in the genetic breeding of Yunnan red radish, a special resource

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