Antibacterial food fresh-keeping Pickering emulsion and preparation method thereof
A food preservation and emulsion technology, which is applied in food preservation, food ingredients as antimicrobial preservation, food ingredients, etc., can solve the problems of poor emulsion stability and affecting the food safety of fruits and vegetables, and achieve the value of preventing corruption and mass production , broaden the effect of the application
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Embodiment 1
[0021] Dissolve 1.25g of B-type gelatin in 25mL of distilled water, stir at 50°C to form a gelatin solution, add 25mL of ethanol, remove the supernatant, redissolve the white precipitate in 15mL of distilled water and adjust the pH value to 12.0, add dropwise ethanol again until Produce white precipitate, then add 625 μ L genipin aqueous solution (5%) to the solution and continue 50 ℃ constant temperature reaction 3~10 hours, the solution after reaction is refrigerated and centrifuged 30 minutes under the condition of 10000g, obtains gelatin nanoparticle, will It was dissolved in distilled water, and its solid content was measured for later use.
[0022] Take 8 mL of sodium periodate (0.5 M) solution and add 10 mL of sodium alginate solution (1 g, 10% w / v), and stir at room temperature for 4 hours in the dark. Add 10 mL of ethylene glycol solution to terminate the reaction. Afterwards, the solution was intensively dialyzed against deionized water and lyophilized to obtain the...
Embodiment 2
[0025] Dissolve 1.25g of B-type gelatin in 25mL of distilled water, stir at 50°C to form a gelatin solution, add 25mL of ethanol, remove the supernatant, redissolve the white precipitate in 15mL of distilled water and adjust the pH value to 12.0, add dropwise ethanol again until Produce a white precipitate, then add 1.25mL genipin aqueous solution (10%) to the solution and continue the constant temperature reaction at 50° C. for 3 to 10 hours, and freeze and centrifuge the reacted solution under 10000 g for 30 minutes to obtain gelatin nanoparticles. Dissolve it in distilled water and measure its solid content for later use.
[0026] Take 8 mL of sodium periodate (0.5 M) solution and add 10 mL of cellulose solution (1 g, 10% w / v), and stir at room temperature for 4 hours in the dark. Add 10 mL of ethylene glycol solution to terminate the reaction. Afterwards, the solution was intensively dialyzed against deionized water and lyophilized to obtain the oxidized polysaccharide. ...
Embodiment 3
[0029] Dissolve 1.25g of B-type gelatin in 25mL of distilled water, stir at 50°C to form a gelatin solution, add 25mL of ethanol, remove the supernatant, redissolve the white precipitate in 15mL of distilled water and adjust the pH value to 12.0, add dropwise ethanol again until Produce white precipitate, then add 625 μ L genipin aqueous solution (5%) to the solution and continue 50 ℃ constant temperature reaction 3~10 hours, the solution after reaction is refrigerated and centrifuged 30 minutes under the condition of 10000g, obtains gelatin nanoparticle, will It was dissolved in distilled water, and its solid content was measured for later use.
[0030] Take 8 mL of sodium periodate (0.5 M) solution and add 10 mL of carrageenan solution (1 g, 10% w / v), and stir at room temperature for 4 hours in the dark. Add 10 mL of ethylene glycol solution to terminate the reaction. Afterwards, the solution was intensively dialyzed against deionized water and lyophilized to obtain the oxi...
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