A kind of preparation method and application of fluorescent molecularly imprinted silica nanoparticles

Abstract

The invention discloses a preparation method and application of fluorescent molecularly imprinted silica gel nanoparticles, which belong to the field of analytical chemistry and relate to the synthesis of molecularly imprinted silica gel polymers. The preparation method comprises the following steps: adding a molecular imprinted pre-polymerization solution prepared by a fluorescent monomer, a functional monomer, a template molecule, a cross-linking agent and a solvent into a reaction vessel; Polymerization is carried out by stirring and reacting to obtain nanoparticles, centrifuged, washed and dried to obtain fluorescent silica nanoparticles containing template molecules; the template molecules in the fluorescent silica nanoparticles containing template molecules can be removed by an eluting solution. The present invention adopts silylating reagents containing amino functional groups as functional monomers, catalysts and fluorescent group immobilization reagents, which not only are easy to prepare, but also have mild reaction conditions, and can eliminate the adverse effects of acidic and basic catalysts on molecular imprinting. Molecularly imprinted silica nanoparticles can be used for fluorescent sensing analysis of target template molecules.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to the synthesis of molecularly imprinted silica gel polymers, in particular to a preparation method and application of fluorescent molecularly imprinted silica gel nanoparticles. Background technique [0002] Molecularly Imprinted Polymer (MIP) is a high molecular polymer with special molecular recognition ability. The formation principle of its selective recognition ability is: firstly, the template molecule and the functional Covalently interact to form a complex, then add a cross-linking agent and an initiator to obtain a molecularly imprinted pre-polymerization solution; then, initiate a polymerization reaction to obtain a high molecular weight polymer containing template molecules; finally, remove the template molecules in the polymer, so that in An imprinted cavity complementary to the shape of the template molecule will be left in the polymer, and the functional group of the...

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Problems solved by technology

However, these methods either require complex preparation processes or the synthesis of special polymerizable fluorescent monomers, and almost all polymerization reactions require initiators, which can severely negatively affect the imprinting efficiency.

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