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Method for preparing bitter-free plant oligopeptide

A non-bitter, plant-based technology, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., to achieve the effects of high yield, sufficient raw materials, and simple process

Inactive Publication Date: 2021-02-05
肽子生物科技(武汉)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through searching, we found that there is no literature report that industrial production can be obtained by enzymatic method, the yield is high, and the amino acid composition ratio of plant oligopeptides (17 kinds of amino acids required by the human body) is not changed, and the beneficial physiological properties of plant oligopeptides are not destroyed. Production method of functional non-bitter plant oligopeptide

Method used

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  • Method for preparing bitter-free plant oligopeptide
  • Method for preparing bitter-free plant oligopeptide

Examples

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Effect test

Embodiment 1

[0017] Plant proteolytic enzyme Promatex, with an enzyme activity of 72,000 U / g, and Flavourzyme enzyme, with an enzyme activity of 500 LAPU / g, are industrial food and proteases.

[0018] A method for preparing non-bitter plant oligopeptides: select food-grade vegetable protein as a raw material, add enzyme hydrolysis, wherein the enzyme is composed of plant proteolytic enzyme and flavor enzyme in a weight ratio of 5:3, and the dosage is 0.6% of the weight of vegetable protein , during the hydrolysis process, the pH of the hydrolyzate is maintained at 8.5 by adding acid and alkali, the degree of hydrolysis is 40%, the enzymolyzate is clarified by high-speed centrifugation, the enzymolysis time is 7 hours, the membrane is finely separated, concentrated, sterilized, and spray-dried to obtain no Bitter plant oligopeptide solid powder. Monitor the temperature at any time through the thermometer used in industrial production, and then add fuel at any time to keep the temperature co...

Embodiment 2

[0023] Plant proteolytic enzyme Promatex, with an enzyme activity of 72,000 U / g, and Flavourzyme enzyme, with an enzyme activity of 500 LAPU / g, are industrial food and proteases.

[0024] A method for preparing non-bitter plant oligopeptides: select food-grade vegetable protein as a raw material, add enzyme hydrolysis, wherein the enzyme is composed of plant proteolytic enzyme and flavor enzyme in a weight ratio of 4:3, and the dosage is 0.8% of the weight of vegetable protein During the hydrolysis process, the pH of the hydrolyzate is maintained at 9.0 by adding acid and alkali, the degree of hydrolysis is 60%, the enzymolyzate is clarified by high-speed centrifugation, and the enzymolysis time is 8 hours; the membrane is finely separated, concentrated and sterilized to obtain plant oligosaccharides without bitter taste Peptide liquid-like product. Monitor the temperature at any time through the thermometer used in industrial production, and then add fuel at any time to keep ...

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Abstract

The invention discloses a production method for preparing enzymolysis plant oligopeptide of bitter-free plant oligopeptide. The method comprises the following steps of selecting food-grade plant protein as a raw material, adding enzyme for hydrolysis, adding acid and alkali in the hydrolysis process to maintain the pH value of a hydrolysate at 9.0+ / -0.5 and the hydrolysis degree at 40%-60%, centrifugally clarifying the hydrolysate at a high speed for 7-8 hours, finely separating a membrane, performing concentration and sterilization, and performing spray drying to obtain the bitter-free plantoligopeptide. The temperature is monitored at any time through a thermometer used in industrial production, and then fuel is supplemented at any time, so that the temperature is kept unchanged, and the enzymolysis time is prolonged to 7-8 hours. According to the method, the annual output can reach 200 tons, and the yield of obtained small molecular active peptides reaches up to 70-90%.

Description

technical field [0001] The invention relates to the application of enzymatic hydrolysis and a production method of plant oligopeptides, in particular to a production method of enzymolysis plant oligopeptides for preparing non-bitter taste plant oligopeptides. Background technique [0002] Peptides are linear polymers of amino acids. Those containing more than 50 amino acid residues are usually called proteins, and those with less than 50 amino acid residues are called peptides. The simplest peptide consists of two amino acid residues, called dipeptides, and peptides containing 3, 4, and 5 amino acid residues are called tripeptides, tetrapeptides, and pentapeptides, respectively. Generally, peptide chains containing several to a dozen amino acid residues are collectively called oligopeptides, and longer peptide chains are called polypeptides (polypeptides). Some scholars refer to those with more than 12 but not more than 20 amino acid residues as oligopeptides, and those wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 王文勋王瑶
Owner 肽子生物科技(武汉)有限公司
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