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Application of group of diagnostic markers in diagnosis of asthenospermia caused by varicocele

A technology of varicocele and diagnostic markers, which is applied in the field of clinical examination and diagnosis, can solve the problems that there are no specific indicators for the diagnosis of varicocele asthenozoospermia and cannot detect asthenospermia, and achieve high sensitivity, highly specific effect

Active Publication Date: 2021-02-12
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Currently, asthenospermia is mainly diagnosed clinically by measuring the number of sperm, combined with seminal parameters such as sperm motility and morphology, at least twice; and the seminal parameter examination is limited to reproductive tract infection , chromosomal abnormalities and other causes, there is no specific index for the diagnosis of asthenozoospermia caused by varicocele, that is to say, the existing detection methods can only detect whether the patient is sick, but cannot detect the cause of varicocele asthenozoospermia

Method used

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  • Application of group of diagnostic markers in diagnosis of asthenospermia caused by varicocele
  • Application of group of diagnostic markers in diagnosis of asthenospermia caused by varicocele
  • Application of group of diagnostic markers in diagnosis of asthenospermia caused by varicocele

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening of potential biomarkers in patients with asthenozoospermia caused by varicocele

[0021] 1. Basic information of the sample

[0022] Patients with varicocele (DR ≥ 1.8mm) and asthenozoospermia (sperm concentration ≥ 15×10) were collected in the first outpatient clinic without medication history and other obvious clinical causes (chromosomal abnormalities, infection, obesity, and environmental factors) were excluded. 6 semen samples / ml, total sperm motility 32%" refers to the percentage of the number of forward motility sperm in the total number of sperm, abbreviated as PR.

[0023] Normal spermatic vein (DR6 / ml, PR+NP>40% and PR>32%) male semen as "normal control group", sample number n=28.

[0024] Extraction of varicocele (DR≥1.8mm) and normal semen parameters (sperm concentration≥15×10 6 men / ml, PR+NP>40% and PR>32%) were taken as "varicocele group", and the number of samples was n=30.

[0025] 2. Instruments and methods

[0026] 2.1 Instrume...

Embodiment 2

[0050] Example 2 Analysis and verification of potential biomarkers in patients with asthenozoospermia caused by varicocele

[0051] Training set samples: including 22 cases of normal control group and 26 cases of varicocele and asthenozoospermia group. Each sample of the varicocele and asthenospermia group should meet the following conditions: varicocele (DR ≥ 1.8 mm) and asthenozoospermia (sperm concentration ≥ 15×10 6 Semen samples / ml, PR+NP6 men / ml, PR+NP>40% and PR>32%) of male semen.

[0052] Test set samples: including 12 cases of normal control group and 15 cases of varicocele and asthenozoospermia group. Each sample of the varicocele and asthenospermia group should meet the following conditions: varicocele (DR ≥ 1.8 mm) and asthenozoospermia (sperm concentration ≥ 15×10 6 Semen samples / ml, PR+NP6 men / ml, PR+NP>40% and PR>32%) of male semen.

[0053] Using the receiver operating curve (ROC) to consider the four diagnostic markers lactic acid, methylmaleic acid, suc...

Embodiment 3

[0059] Example 3 Kit and detection instrument for diagnosing asthenozoospermia caused by varicocele

[0060] A kit for the diagnosis of asthenospermia due to varicocele, including reagents for the detection of lactic acid, methylmaleic acid, succinic acid and asparagine.

[0061] A testing instrument for diagnosing asthenozoospermia caused by varicocele, which can detect the contents of lactic acid, methylmaleic acid, succinic acid and asparagine in semen.

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Abstract

The invention provides an application of a group of diagnostic markers in diagnosis of asthenospermia caused by varicocele, and belongs to the field of clinical examination and diagnosis. Specifically, the invention provides the application of the diagnostic markers in preparation of a kit and a detection instrument for diagnosing asthenospermia caused by varicocele, and the diagnostic markers arelactic acid, methyl maleic acid, succinic acid and asparagine. Lactic acid, methyl maleic acid, succinic acid and asparagine are combined to diagnose asthenospermia caused by varicocele, and the kithas the advantages of being high in specificity and sensitivity and only needing trace samples. Meanwhile, lactic acid, methyl maleic acid, succinic acid and asparagine are detected, the asthenospermia caused by varicocele can be rapidly diagnosed, and an important basis is provided for early warning, clinical diagnosis, treatment and prognosis evaluation of the asthenospermia caused by varicocele.

Description

technical field [0001] The invention belongs to the field of clinical examination and diagnosis, and in particular relates to the application of a group of diagnostic markers in the diagnosis of asthenozoospermia caused by varicocele. Background technique [0002] Asthenozoospermia is the main cause of male infertility. Both epidemiological and clinical studies have confirmed that men with varicocele have a greatly increased risk of asthenozoospermia. [0003] At present, asthenozoospermia is mainly diagnosed clinically by measuring the number of sperm, combined with semen parameters such as sperm motility and morphology, and at least two inspections; and the semen parameter inspection is limited to reproductive tract infection, chromosomal abnormalities and other causes, and there is no diagnosis of varicocele. Specific indicators for the diagnosis of asthenozoospermia, that is to say, the existing detection method can only detect whether the patient is suffering from the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8634
Inventor 张琪周艳芬马博黄涛
Owner NANJING UNIV OF TECH
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