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Donkey milk-derived Pediococcus pentosaceus and application thereof

A technology of Pediococcus pentosaceae and donkey milk, which is applied in the direction of application, bacteria, and dairy products, can solve the problems of yogurt prepared by Pediococcus pentosaceae, so as to alleviate lactose intolerance and bacterial diarrhea, improve Effects of intestinal micro-ecological environment and good health function

Active Publication Date: 2021-02-19
新疆灵水生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this patent does not disclose the characteristics of yogurt prepared by Pediococcus pentosaceae, and the prepared yogurt can alleviate lactose intolerance, prevent and treat diarrhea, etc.

Method used

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  • Donkey milk-derived Pediococcus pentosaceus and application thereof
  • Donkey milk-derived Pediococcus pentosaceus and application thereof
  • Donkey milk-derived Pediococcus pentosaceus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Screening and Identification of Example 1 Bacterial Strains

[0049] 1.1 Isolation and purification of strains

[0050] First, the improved MRS (containing 0.5g / L cysteine) medium was prepared and sterilized, and poured onto the plate until it was solidified, then the donkey milk sample collected from the Hami area of ​​Xinjiang was diluted with sterile normal saline, and the Dilution gradient of 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 100 μL of diluent was sucked and coated on the solidified plate, and the number of the plate was recorded, and the coated plate was placed in an anaerobic box at 37°C and incubated upside down for 36 hours.

[0051] For each donkey milk sample, select a flat plate with appropriate dilution to pick single colonies with different colors, sizes and shapes for microscopic examination, keep the colonies with rod-shaped or spherical cells, and continuously transfer and streak culture for 3 times, and then pick The pure single colony is placed o...

Embodiment 2

[0066] Example 2 Study on the probiotic properties of Pediococcus pentosaceae HMTN.01

[0067] 2.1 Acid tolerance and bile salt tolerance of Pediococcus pentosaceae HMTN.01

[0068] (1) Determination of acid tolerance of Pediococcus pentosaceae HMTN.01

[0069] After activation, Pediococcus pentosaceae HMTN.01 was inoculated in liquid MRS medium with an inoculum of 1%, and after static culture at 37°C for 18 hours, the bacterial concentration was about 4.8×10 8 CFU / mL, after centrifugation at 4000r / min for 10min, the bacteria were collected, washed twice with sterile saline, and then resuspended in liquid MRS medium at pH 2.5. At 0, 1, 2, and 3 hours, draw 100 μL of the bacterial suspension and use sterile saline for gradient dilution, and select an appropriate dilution to count the colonies on the plate. The strain in the control group was Streptococcus thermophilus D-2, and the acid tolerance experiment was the same as above, and all were parallelized 3 times.

[0070] (2...

Embodiment 3

[0108] The determination of embodiment 3 bacterial strain drug resistance

[0109] Both the resistance and infection ability of probiotics to antibiotics have a great impact on human safety. When probiotics antagonize pathogenic bacteria, if the drug resistance gene is transferred to the pathogenic bacteria, the pathogenic bacteria will develop resistance to the corresponding antibiotics. While lactic acid bacteria are widely recognized as safe, their safety needs to be evaluated.

[0110] According to the drug susceptibility test method recommended by WHO, the drug susceptibility test of 20 kinds of antibiotics was carried out on Pediococcus pentosaceae HMTN.01 by Kirby-Bauer method (K-B disc diffusion method). Referring to NCCLS regulations, dilute the inoculum to 10 7 ~10 8 CFU / mL, take 120 μL of the bacterial suspension on the surface of the MRS agar plate, spread it evenly, and stick the tablet on the surface with sterile tweezers after the surface of the plate is dry....

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Abstract

The invention belongs to the technical field of microbial fermentation, and particularly relates to a donkey milk-derived Pediococcus pentosaceus strain and application thereof. The Pediococcus pentosaceus HMTN.01 is collected in China Center for Type Culture Collection on May 25, 2020, and the collection number is CCTCC NO: M 2020144. The strain has the advantages of strong acid production, viscosity production and fragrance production capabilities, better hydrophobicity, self-aggregation capability and gastrointestinal tolerance capability, can generate better adhesion to the gastrointestinal tract, is favorable for field planting in the gastrointestinal tract, can inhibit intestinal pathogenic bacteria and regulate intestinal flora, and can be prepared into food or food additives. The yogurt prepared by adopting the strain has good flavor, taste and appearance, and can be used for large-scale preparation of yogurt.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a donkey milk-derived Pediococcus pentosacea and an application thereof. Background technique [0002] Yogurt is a kind of milk product that uses fresh milk or reconstituted milk as the raw material, after pasteurization, adding starter to the milk, fermenting at about 42°C, and then cooling and filling. At present, there are various yogurts on the market, which are very popular among consumers for their unique flavor, comfortable taste, rich nutrition and good health care functions. With the public's pursuit of a healthy life and changes in consumption concepts, the consumption of various yogurts is also growing continuously, and the yogurt industry has become a new growth spot in the dairy industry. The quality and flavor of yogurt depend on the quality, type and vitality of the lactic acid bacteria starter. The production performance (acid producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/744A61P31/04A23L33/135A23C9/123C12R1/01
CPCA61K35/744A61P31/04A23L33/135A23C9/123A23V2002/00C12R2001/01C12N1/205A23V2200/3204Y02A50/30
Inventor 倪永清杨博柏吉靳亚梅李谞张艳
Owner 新疆灵水生物科技有限公司
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