Application of protea cynaroides extract in preparation of skin anti-aging composition

A technology of Putia flowers and extracts, applied in the field of plant extracts, can solve the problems of skin sensitivity, delay skin aging, and inability to directly and effectively improve water retention capacity, so as to improve mitochondrial activity, maintain vitality and normal metabolism , enhance the effect of anti-aging activity

Inactive Publication Date: 2021-02-23
百岳特生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, most of the skin anti-aging products on the market can only increase the antioxidant activity, and cannot directly and effectively improve or delay the occurrence of skin aging
[0005] Based on the above, in order to improve the skin’s fragility and sensitivity caused by damaged keratinocytes, decreased water retention capacity, and skin aging, it is necessary to develop a method that can directly and effectively improve or delay the occurrence of skin aging, while at the same time It is necessary to effectively maintain the normal physiological metabolism of keratinocytes and maintain the integrity of the stratum corneum structure

Method used

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  • Application of protea cynaroides extract in preparation of skin anti-aging composition
  • Application of protea cynaroides extract in preparation of skin anti-aging composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]Example 1 Method for Preparation of Putihua Extract of the present invention

[0033]Protea Cynaroides is a perennantnometric plant, which is a PROTEACEAE. Also known as the name of the emperor, the sea god, the dragon flower, etc., which is the national flower of the South African Republic. The flower is huge, the flower is grateful, and the flowers of Putia are dry. After drying, it can be bifpected into flower tea, which has the effect of promoting digestion, relaxing nerve, sweating diuretic, lowering blood pressure, and can also reduce cold fever, alleviate nasal mucosa And sore throat.

[0034]In an embodiment of the present invention, Putia flower is washed, and the purified Putihua and water, an alcohol, or an alcohol moisture mixture are extracted, and the extraction solvent is preferably water, at 1- The weight ratio of 5: 10-20 was mixed. After the homogeneous extract was extracted from 50 to 100 ° C, the crude extract was cooled to room temperature, and the crude extract...

Embodiment 2

[0035]Example 2 The Putihua Extract of Putihua Extracts in the present invention promotes the efficacy of elastic protein proliferation

[0036]An embodiment of the present invention is tested in the pre-promotion of elastic protein proliferation by human skin fibrobromLAST, CCD-966SK. The human skin fiber parent cell is purchased from the American Typical Culture Safety CenterThe cell number was CRL-1881 which was cultured in a culture solution containing 10% fetal bovine serum (Fetal Bovine Serum) and 90% Minimum Essentialmedium (MEM, purchased from Gibco, USA). Among them, 1 mM sodium pyruvate (Sodium Pyruvate, purchased from GIBCO, USA) and 1% penicillin / streptomycin (Penicillins / Streptomycin, purchased from Gibco, USA).

[0037]First, 1 × 105Personal skin fibrosis is planted in a 6-well culture plate containing 2 mL of the above culture solution, and after 24 hours at 37 ° C, the cell culture solution is removed, and the cell culture fluid is removed, and the phosphoric acid is 1...

Embodiment 3

[0039]Example 3 Putihua extract of Putihua extract in the present invention lifts the effect of skin cell mitochondrial activity

[0040]An embodiment of the present invention is based on Human Skin Fibroblast CCD-966SK cells, and BDTMFlow Cytometry Mit ° C Hondrial Membranepotential Detection Kit, to carry out the effect of promoting the activity of skin cell mitochondrial activity in Putihua extract of the present invention. Among them, the CCD-966SK cells are purchased from Biological Resources Preservation and Research Center (BiorellCollection and Research Center, BCRC, China Taiwan), numbered BCRC No. 60153, and this cell is cultured to 10% fetal bovine serum (Fetal Bovine Serum) and 90% of the cell culture medium of Eagle's Minimumessential Medium (EMEM) (purchased from GIBCO, US), and containing 0.1 mm non-essential amino acids, 1.5g / L Sodium hydrogen bicarbonate, and 1 mM sodium pyruvate.

[0041]Where used this BDTMBefore the Mitcreen (JC-1) reagent set, the 10X JC-1 dyeing me...

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Abstract

The invention relates to the field of plant extracts, in particular to an application of a protea cynaroides extract in preparation of a skin anti-aging composition. The invention provides an application of a protea cynaroides extract in preparation of a skin anti-aging composition, and the protea cynaroides extract can effectively improve the elastin secretion amount of skin cells and maintain the skin compact and elastic so as to reduce wrinkles of the skin, and can improve the mitochondrial activity of the skin cells so that the activity and normal metabolism of the skin cells are maintained, and the anti-aging activity of the skin cells is improved.

Description

Technical field[0001]The present invention relates to the field of plant extracts, in particular about a composition for preparing skin anti-aging, in particular a Putihua extract.Background technique[0002]The skin layer is the outermost layer of the skin, which is internally internally, the particulate layer, the ratty of the substrate layer, and the skin layer is mainly differentiated from the undifferentiated cylindrical keratin in the base layer. The process is called hornification. High water content in keratinocytes is high, and as the cells are differentiated, the shape of the keratinocytes will gradually become flat, and the nuclei and the orcharge start degradation and shrinking, and the keratinic layer is formed without a nuclear nuclear cytoplate. Dead cells. The main function of the epidermis layer is to keep the skin and form a skin barrier to protect against various foreign injuries, wherein the outermost layer of the epidermal layer is composed of a weak acidic sebum ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P17/00A61P17/18A23L33/105
CPCA23V2002/00A23L33/135A61K35/747A61P17/00A61K8/99A61Q19/02A61Q19/00A61K2800/782A23V2200/318A61K35/66A61P3/04A61P3/08C12N1/18C12R2001/865C12N1/185
Inventor 林咏翔李姿仪
Owner 百岳特生物技术(上海)有限公司
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