Application of protea cynaroides extract in preparation of skin anti-aging composition
A technology of Putia flowers and extracts, applied in the field of plant extracts, can solve the problems of skin sensitivity, delay skin aging, and inability to directly and effectively improve water retention capacity, so as to improve mitochondrial activity, maintain vitality and normal metabolism , enhance the effect of anti-aging activity
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Embodiment 1
[0032]Example 1 Method for Preparation of Putihua Extract of the present invention
[0033]Protea Cynaroides is a perennantnometric plant, which is a PROTEACEAE. Also known as the name of the emperor, the sea god, the dragon flower, etc., which is the national flower of the South African Republic. The flower is huge, the flower is grateful, and the flowers of Putia are dry. After drying, it can be bifpected into flower tea, which has the effect of promoting digestion, relaxing nerve, sweating diuretic, lowering blood pressure, and can also reduce cold fever, alleviate nasal mucosa And sore throat.
[0034]In an embodiment of the present invention, Putia flower is washed, and the purified Putihua and water, an alcohol, or an alcohol moisture mixture are extracted, and the extraction solvent is preferably water, at 1- The weight ratio of 5: 10-20 was mixed. After the homogeneous extract was extracted from 50 to 100 ° C, the crude extract was cooled to room temperature, and the crude extract...
Embodiment 2
[0035]Example 2 The Putihua Extract of Putihua Extracts in the present invention promotes the efficacy of elastic protein proliferation
[0036]An embodiment of the present invention is tested in the pre-promotion of elastic protein proliferation by human skin fibrobromLAST, CCD-966SK. The human skin fiber parent cell is purchased from the American Typical Culture Safety CenterThe cell number was CRL-1881 which was cultured in a culture solution containing 10% fetal bovine serum (Fetal Bovine Serum) and 90% Minimum Essentialmedium (MEM, purchased from Gibco, USA). Among them, 1 mM sodium pyruvate (Sodium Pyruvate, purchased from GIBCO, USA) and 1% penicillin / streptomycin (Penicillins / Streptomycin, purchased from Gibco, USA).
[0037]First, 1 × 105Personal skin fibrosis is planted in a 6-well culture plate containing 2 mL of the above culture solution, and after 24 hours at 37 ° C, the cell culture solution is removed, and the cell culture fluid is removed, and the phosphoric acid is 1...
Embodiment 3
[0039]Example 3 Putihua extract of Putihua extract in the present invention lifts the effect of skin cell mitochondrial activity
[0040]An embodiment of the present invention is based on Human Skin Fibroblast CCD-966SK cells, and BDTMFlow Cytometry Mit ° C Hondrial Membranepotential Detection Kit, to carry out the effect of promoting the activity of skin cell mitochondrial activity in Putihua extract of the present invention. Among them, the CCD-966SK cells are purchased from Biological Resources Preservation and Research Center (BiorellCollection and Research Center, BCRC, China Taiwan), numbered BCRC No. 60153, and this cell is cultured to 10% fetal bovine serum (Fetal Bovine Serum) and 90% of the cell culture medium of Eagle's Minimumessential Medium (EMEM) (purchased from GIBCO, US), and containing 0.1 mm non-essential amino acids, 1.5g / L Sodium hydrogen bicarbonate, and 1 mM sodium pyruvate.
[0041]Where used this BDTMBefore the Mitcreen (JC-1) reagent set, the 10X JC-1 dyeing me...
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