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Idl7 mature polypeptide plant aging accelerator, its preparation method and application

A mature polypeptide and IDL7 technology, applied in botany equipment and methods, plant growth regulators, plant growth regulators, etc., can solve the problem of no plant senescence, etc., and achieve convenient operation, strong operability in the field, and beneficial effects Effect

Active Publication Date: 2021-09-17
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies on the application of mature polypeptides in plants, especially crops, and there is no research on their application in promoting plant senescence.

Method used

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  • Idl7 mature polypeptide plant aging accelerator, its preparation method and application
  • Idl7 mature polypeptide plant aging accelerator, its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] IDL7 mature polypeptide plant senescence accelerator, comprises 10 μ mol / L IDL7 mature polypeptide, 2.8mmol / L 2-(N-morpholine) ethanesulfonic acid solution; Described 2-(N-morpholine) ethanesulfonic acid solution is 2- (N-morpholine)ethanesulfonic acid solution monohydrate was dissolved in MS liquid medium, and the pH of 2-(N-morpholine)ethanesulfonic acid solution was 5.8.

[0030] The preparation method is as follows:

[0031] (1) Weigh the IDL7 mature polypeptide powder and dissolve it in water, and prepare the IDL7 mature polypeptide mother solution with a concentration of 10mmol / L for subsequent use;

[0032] (2) Weigh quantitative 2-(N-morpholine)ethanesulfonic acid monohydrate, add it to the above-mentioned MS liquid medium, prepare a 2.8mmol / L solution, adjust the pH value of the solution to 5.8, mix and dissolve Obtain 2-(N-morpholine) ethanesulfonic acid solution evenly;

[0033] (3) Add 40 μL of the IDL7 mature polypeptide mother liquor obtained in step (1)...

Embodiment 2

[0035] IDL7 mature polypeptide plant senescence accelerator comprises 12 μ mol / L IDL7 mature polypeptide, 2.9mmol / L 2-(N-morpholine) ethanesulfonic acid solution; Described 2-(N-morpholine) ethanesulfonic acid solution is 2- (N-morpholine)ethanesulfonic acid solution monohydrate was dissolved in MS liquid medium, and the pH of 2-(N-morpholine)ethanesulfonic acid solution was 5.9.

[0036] The preparation method is as follows:

[0037] (1) Weigh the IDL7 mature polypeptide powder and dissolve it in water, and prepare the IDL7 mature polypeptide mother solution with a concentration of 10mmol / L for subsequent use;

[0038](2) Weigh quantitative 2-(N-morpholine)ethanesulfonic acid monohydrate, add it to the above MS liquid medium, prepare a 2.5mmol / L solution, adjust the pH value of the solution to 5.9, mix and dissolve Uniformly obtain 2-(N-morpholine) ethanesulfonic acid solution;

[0039] (3) Take 120 μL of the IDL7 mature polypeptide mother liquor obtained in step (1) and ad...

Embodiment 3

[0041] IDL7 mature polypeptide plant senescence accelerator, including 13 μmol / L IDL7 mature polypeptide, 3mmol / L2-(N-morpholine) ethanesulfonic acid solution; Described 2-(N-morpholine) ethanesulfonic acid solution is 2-(N -Morpholine)ethanesulfonic acid solution monohydrate is dissolved in MS liquid medium, and the pH of 2-(N-morpholine)ethanesulfonic acid solution is 5.8.

[0042] The preparation method is as follows:

[0043] (1) Weigh the IDL7 mature polypeptide powder and dissolve it in water, and prepare the IDL7 mature polypeptide mother solution with a concentration of 10mmol / L for subsequent use;

[0044] (2) Weigh quantitative 2-(N-morpholine)ethanesulfonic acid monohydrate, add it to the above MS liquid medium, prepare a 3mmol / L solution, adjust the pH value of the solution to 5.8, mix and dissolve evenly Obtain 2-(N-morpholine)ethanesulfonic acid solution;

[0045] (3) Take 78 μL of the IDL7 mature polypeptide mother solution obtained in step (1) and add it to 6...

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Abstract

The present invention proposes an IDL7 mature polypeptide plant aging accelerator, its preparation method and application, and belongs to the field of plant aging accelerators. The IDL7 mature polypeptide plant aging accelerator uses IDL7 mature polypeptide as the main functional component. The IDL7 mature polypeptide has the following properties: Amino acid sequence: F‑G‑S‑L‑V‑L‑N‑A‑L‑P‑K‑G‑S‑R‑P‑G‑S‑G‑P‑S‑K‑K‑T‑N . The plant senescence accelerator provided by the invention can accelerate the senescence of leaves of plants and accelerate the senescence without other additional adverse performances, and has strong operability in the field.

Description

technical field [0001] The invention belongs to the field of plant aging accelerators, and in particular relates to an IDL7 mature polypeptide plant aging accelerator, its preparation method and application. Background technique [0002] Leaf senescence is an important factor affecting crop yield, quality and other agronomic traits. Taking tobacco as an example, the leaves are the harvesting organs of tobacco. As the tobacco enters the late growth stage, the leaves enter the aging stage, and the maturity stages of the upper, middle, and lower leaves of tobacco are different. Harvest time, and the use of aging accelerators can effectively solve the problems of inconsistent maturity and greening of tobacco leaves in different parts. [0003] At present, the application of plant aging control mainly focuses on the regulation of plant hormones. For example, auxin and cytokinin can inhibit leaf aging, while ethylene and abscisic acid can promote leaf aging. aging process. Sene...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/46A01N43/80A01P21/00
CPCA01N37/46A01N43/80A01P21/00A01H3/04C07K14/415C07K14/001
Inventor 郭永峰郭存王奇李伟张增林文利超邓智超刘涛
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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