Hepatocyte three-dimensional culture device with simulated hepatic plate structure

Abstract

The invention discloses a hepatocyte three-dimensional culture device with a simulated hepatic plate structure, and relates to the technical field of hepatocyte culture. The hepatocyte three-dimensional culture device specifically comprises a hepatocyte culture box, anti-blocking channel assemblies and pipeline channel assemblies, a gelatinizing groove is formed in the middle of the hepatocyte culture box, and the anti-blocking channel assemblies are buried in the two sides of the hepatocyte culture box; and the pipeline channel assemblies are arranged on the surfaces of the two sides of the hepatocyte culture box, a culture solution conveying pipe penetrates through the interior of a rubber ring sleeve, a solution outlet is formed in the surface, close to a metal abutting head, of the left end of the culture solution conveying pipe, and an anti-retreating rubber ring is fixed to the surface of the right side, close to the solution outlet, of the left end of the culture solution conveying pipe. According to the hepatocyte three-dimensional culture device with the imitated hepatic plate structure, a rotating impeller can push a culture solution to continuously advance in a culture solution channel through rotation of a variable speed motor, pause and solidification of the culture solution are avoided, blockage in the culture solution channel is avoided accordingly, the rotatingspeed can be adjusted through the variable speed motor, and therefore the flowing speed of the culture solution is controlled.

Description

technical field [0001] The invention relates to the technical field of hepatocyte culture, in particular to a three-dimensional culture device for hepatocytes with an imitation liver plate structure. Background technique [0002] The three-dimensional culture device of liver cells with imitation liver plate structure is to simulate the three-dimensional environment of liver cells in the body for cultivation in vitro. It is an important device for the purpose of liver function repair and replacement, and is widely used in the medical industry. [0003] The existing three-dimensional hepatocyte culture device with imitation liver plate structure needs to sequentially inject various culture solutions (sodium alginate solution and gel solution containing cells) through several pipelines to the gelation area to react, but because the culture solution is The colloid is very easy to stop the flow in the pipeline and block it, which is not conducive to the continuation of the cultiv...

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Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a three-dimensional hepatic cell culture device with a liver-like plate structure, which solves the problem that the existing three-dimensional culture device with a liver-like plate structure needs to be injected into each of the three-dimensional culture devices through several pipelines sequentially during use. The culture solution (cell-containing sodium alginate solution and gelling solution) reacts to the gel-forming area, but because the culture solution is gelatinous, it is easy to stop the flow in the pipeline and cause blockage, which is not conducive to the continuation of the cultivation operation.

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