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Probiotic composition for inhibiting helicobacter pylori and application

A technology of Helicobacter pylori and composition, applied in the field of probiotic composition for inhibiting Helicobacter pylori

Active Publication Date: 2021-03-09
JIAXING INNOCUL PROBIOTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the selection of probiotic strains for the treatment of Hp and the synergistic effect of different strain compositions in the treatment of Hp still need to be confirmed by a large number of further studies.

Method used

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  • Probiotic composition for inhibiting helicobacter pylori and application
  • Probiotic composition for inhibiting helicobacter pylori and application
  • Probiotic composition for inhibiting helicobacter pylori and application

Examples

Experimental program
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preparation example Construction

[0024] In the probiotic composition of the present invention, the weight part of the Lactobacillus rhamnosus is preferably 15-25 parts, more preferably 20 parts. The Lactobacillus rhamnosus of the present invention preferably includes Lactobacillus rhamnosus LN56, and the preservation number is CGMCC No.17370. The number of viable bacteria in the bacterial powder of Lactobacillus rhamnosus LN56 of the present invention is preferably 1.0 × 10 10 ~3.0×10 11 CFU / g, more preferably 5.0×10 10 ~2.0×10 11 CFU / g, most preferably 8.0×10 10 CFU / g. The preparation method of the bacterial powder of the Lactobacillus rhamnosus LN56 in the present invention is not particularly limited, preferably comprising: 1) inoculating the Lactobacillus rhamnosus LN56 in the MRS culture fluid or the improved MRS culture fluid, at 36 Fermentation at ~38°C for 12 to 24 hours to obtain Lactobacillus rhamnosus LN56 fermentation liquid; the improved MRS culture liquid uses MRS culture liquid as a basal ...

Embodiment 1

[0039] Lactobacillus rhamnosus LN56, Lactobacillus plantarum LN66, Lactobacillus reuteri LN83 and Lactobacillus salivarius LN12 were used to activate facultative anaerobic culture in MSR medium at 37°C for 24 hours, then centrifuged at 10000rpm for 10min to remove bacteria The cell-free fermentation supernatant was obtained, and the inhibitory effects of the cell-free fermentation supernatant (CFS) of a single strain and the CFS composition of the four strains on Helicobacter pylori were detected respectively. Helicobacter pylori (Helicobacter pylori ATCC 43504) was purchased from the American Type Culture Collection. The solid culture of Helicobacter pylori was cultured on Colombian blood agar medium containing 10% sterile defibrinated sheep blood at 37°C under microaerophilic conditions for 3-4 days, and the liquid culture was cultured on Brucella broth medium containing 10% fetal bovine serum at 37°C. Cultivate at 80 rpm for 2-3 days under microaerobic conditions.

[0040]...

Embodiment 2

[0046] The composition of the cell-free fermentation supernatant (CFS) of Lactobacillus rhamnosus LN56, Lactobacillus plantarum LN66, Lactobacillus reuteri LN83, Lactobacillus salivarius LN12 obtained in Example 1 and the CFS of these 4 kinds of bacteria , to detect the effect on gene expression levels of Helicobacter pylori virulence factors CagA, VacA and Ure.

[0047] The specific detection method is as follows: culture Helicobacter pylori (Helicobacter pylori ATCC 43504) in 10% fetal bovine serum Brooke's broth medium for 48-72 hours microaerobically, dilute the bacterial solution with the broth until the bacterial density is 1×10 7 CFU / mL. Add 5mL LN56 CFS, 5mL LN66 CFS, 5mL LN83 CFS, 5mL LN12 CFS, and 5mL equal-proportion compositions (each 1.25mL) of the above four strains of CFS into different conical flasks, and then in each conical flask, respectively Add 5 mL of 10% fetal bovine serum Brucella broth medium and 10 mL of 1×10 7 CFU / mL of Hp bacteria liquid, 37 ℃ mic...

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Abstract

The invention provides a probiotic composition for inhibiting helicobacter pylori and application, and belongs to the technical field of foods and medicines. The probiotic composition comprises four probiotics of lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus reuteri and lactobacillus salivarius. The probiotic composition has a synergistic inhibition effect on the helicobacter pylori, can significantly inhibit the growth of the helicobacter pylori, reduce the gene expression level of virulence factors CagA, VacA and Ure, can turn helicobacter pylori of a helicobacter pylori infection model mouse into negative, and can effectively reduce the number of helicobacter pylori in a human body or turn helicobacter pylori into negative. The four strains of the probiotic compositionhave a mutual synergistic effect, and the helicobacter pylori inhibiting effect of the probiotic composition is remarkably superior to the single application effect of each strain.

Description

technical field [0001] The invention belongs to the technical field of food and medicine, and in particular relates to a probiotic composition for inhibiting Helicobacter pylori and its application. Background technique [0002] Helicobacter pylori (Hp) can cause many digestive tract diseases. It can parasitize the gastric mucosa for a long time and easily cause chronic gastritis, gastric ulcer and even gastric cancer. It is classified as a class I carcinogen by the World Health Organization (WHO). [0003] The standard clarithromycin triple therapy for eradicating H. As well as the increase in the incidence of related side effects such as abdominal distension, diarrhea and constipation caused by antibiotics and bismuth, the radical effect of antibiotic therapy and the patient's tolerance and medication compliance have been greatly reduced. [0004] Probiotic therapy is an emerging therapy for the treatment of Hp. Research and clinical practice in recent years have shown th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P31/04C12R1/225C12R1/25
CPCC12N1/20A23L33/135A61K35/747A61P31/04A23V2002/00A23V2400/173A23V2400/175A23V2400/181A23V2400/169A23V2200/3204A61K2300/00
Inventor 不公告发明人
Owner JIAXING INNOCUL PROBIOTICS CO LTD
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