Column hardware and separation column, and method for manufacturing same
A manufacturing method and hardware technology, applied in separation methods, material separation, chemical instruments and methods, etc., can solve problems such as solute peak shape deformation
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Embodiment 1
[0125] The first membrane made of perhydropolysilazane was prepared in the mobile phase liquid contact part of the stainless steel column hardware of the general-purpose UHPLC column, and then the second membrane was fabricated, that is, the methyl formaldehyde on the surface of the first membrane Silylation.
[0126] Specifically, the amount of two columns was prepared using a column hardware made of stainless steel with a mobile phase-liquid contact portion having a first membrane made of perhydropolysilazane as a raw material. Make the isopropyl alcohol solution (1.0%, w / v) of perhydropolysilazane with a tall cylinder beaker of 1000mL, put stainless steel column hardware (two column tubes with an inner diameter of 2.1mm and a length of 5cm, four pieces of frit , four end unions) sink in. Sonicate for 3 minutes, take out the column hardware, and dry at room temperature for 12 hours on a laboratory bench covered with industrial wipes. Move the dried column hardware into an ...
Embodiment 2
[0134] The first membrane made of methyltriethoxysilane (MTES) was fabricated on the mobile phase liquid contact part of the stainless steel column hardware of a general-purpose UHPLC column, and then the second membrane was fabricated, that is, the surface of the first membrane of methyl silylation.
[0135] Specifically, the amount of one column was produced by using MTES as a raw material in the mobile phase liquid contact part of the column hardware made of stainless steel and forming the first film by chemical vapor deposition. Put stainless steel column hardware (a column tube with an inner diameter of 2.1 mm and a length of 5 cm, two frits, and two end unions) into the vacuum chamber, close the door of the vacuum chamber, and heat the vacuum chamber to keep it at a constant temperature at 300°C. In addition, the vacuum degree of the vacuum chamber was kept at a constant pressure of -0.099 MPa. Next, a gas that dilutes MTES to 0.05% with nitrogen is introduced into the...
Embodiment 3
[0141] The first membrane made of dimethyldiethoxysilane (DMDES) is fabricated in the mobile phase liquid contact part of the stainless steel column hardware of a general-purpose UHPLC column, and then the second membrane is fabricated, that is, the first membrane Methyl silylation of the surface.
[0142] Specifically, the amount of one column was prepared by using DMDES as a raw material in the mobile phase liquid contact part of the column hardware made of stainless steel and forming the first film by chemical vapor deposition. Put stainless steel column hardware (a column tube with an inner diameter of 2.1 mm and a length of 5 cm, two frits, and two end unions) into the vacuum chamber, close the door of the vacuum chamber, and heat the vacuum chamber to keep it at a constant temperature at 300°C. In addition, the vacuum degree of the vacuum chamber was kept at a constant pressure of -0.099 MPa. Next, a gas diluted with nitrogen to 0.05% of DMDES is introduced into the va...
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Abstract
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