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A Trichoderma strain for preventing and controlling wheat powdery mildew and its application

A technology for wheat powdery mildew and Trichoderma, applied in the fields of application, fungicides, fungi, etc., can solve the problems of poor control effect of wheat powdery mildew, affecting wheat yield, etc., and achieve the goal of improving wheat quality, good control effect, and reducing costs Effect

Active Publication Date: 2022-02-15
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the antagonistic microorganisms that have been discovered so far, the biocontrol Trichoderma has been verified as a biocontrol antagonist with broad-spectrum adaptability. It has been found that Trichoderma can produce at least 29 pathogenic fungi in 18 genera in vitro or in vivo. Antagonism, however, these Trichoderma fungi have poor control effects on wheat powdery mildew, and affect the yield of wheat after wheat is infected. Therefore, it is of great significance to provide a Trichoderma fungus with high control efficiency and improved wheat resistance

Method used

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  • A Trichoderma strain for preventing and controlling wheat powdery mildew and its application
  • A Trichoderma strain for preventing and controlling wheat powdery mildew and its application
  • A Trichoderma strain for preventing and controlling wheat powdery mildew and its application

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Effect test

Embodiment 1

[0036] Example 1 strain screening and identification

[0037] The isolation and identification process of Trichoderma is as follows:

[0038] (1) Separation and purification: Weigh 10g of soil sample, pour it into sterilized physiological saline with a volume of 90ml and a mass concentration of 0.85%, shake it at 25°C for 1 hour at 120rpm / min to obtain solution I; dilute solution I gradually 1ml of each gradient was put into a plate, poured into PDA medium at 40°C, mixed evenly, and cultured at 25°C for 8 days. After the strains grew out, they were transferred to a new plate for culture until a single spore colony, preservation;

[0039] The initial growth color is white, and the mycelium layer is thick. As the growth time prolongs, the plate appears green due to the production of conidia, and the mycelium clusters are dense like a carpet; the front of the colony is dark green, and the back is colorless;

[0040](2) Identification: identify the strain purified in step (1), e...

Embodiment 2

[0047] The preparation of embodiment 2 Trichoderma 124D spores

[0048] The preparation process of Trichoderma harzianum 124D spore is as follows:

[0049] (1) Strain activation: the preserved Trichoderma 124D was inoculated in solid PDA medium, and cultured in dark in an incubator at 25°C for 7 days;

[0050] (2) Preparation of spore suspension: wash the solid PDA medium with sterilized water, filter the bacterial solution with gauze, take 10 μl of the filtered bacterial solution with a pipette gun and drop it into a hemocytometer, and use a microscope to Observe the amount of spores, and finally adjust the amount of spores to 1.0×10 8 CFU, obtain Trichoderma harzianum 124D spore suspension;

[0051] (3) Preparation method of spores: use wheat seeds as culture medium, weigh an appropriate amount of wheat seeds and put them into a tissue culture bottle, after high-temperature sterilization at 121°C, stand-by; use the Trichoderma 124D spore suspension obtained in step (2) Pu...

Embodiment 3

[0052] Example 3 Bacteria Preparation 1

[0053] Use Trichoderma harzianum 124D spores to prepare powder, spore content is 1×10 9 CFU / g, the bacterial agent includes the following components in parts by weight:

[0054] 5 parts of Trichoderma harzianum 124D spores, 2.5 parts of sodium dodecylsulfonate, 2.5 parts of xanthan gum, 4 parts of glucose, and 86 parts of diatomaceous earth; mix the above components to obtain a powder.

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Abstract

The present invention provides a strain of Trichoderma for preventing and treating wheat powdery mildew and its application. The Trichoderma is Trichoderma harzianum (Trichoderma harzianum) 124D, which has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on November 18, 2020. The depository unit is referred to as CGMCC, address: Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, and deposit number: CGMCC No.21030. The application of the trichoderma in the prevention and treatment of wheat powdery mildew. The bacterial agent prepared by the Trichoderma harzianum 124D can effectively prevent and control wheat powdery mildew, increase the amino acid and protein content in wheat, and further improve the resistance of wheat itself.

Description

technical field [0001] The invention relates to the technical field of biological applications, in particular to a Trichoderma strain for preventing and treating wheat powdery mildew and its application. Background technique [0002] Wheat is a major food source, and its yield and quality have a major impact on food security. Wheat disease is mainly wheat powdery mildew caused by Blumeriagraminis f.sp.tritici. The spores of the pathogen spread with the airflow, which makes powdery mildew have the characteristics of fast prevalence, wide range of damage, and difficult control. Due to powdery mildew It is seriously harmful to the yield and quality of wheat. Therefore, the prevention and treatment of wheat powdery mildew has become the focus of wheat pathogen research. [0003] In the prior art, the prevention and treatment of wheat powdery mildew is mainly based on chemical control and the use of disease-resistant wheat varieties, supplemented by cultivation and management. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02C12Q1/6895C12Q1/04A01N63/38A01P3/00C12N3/00C12R1/885
CPCC12N1/14C12N1/02C12Q1/6895A01N63/38C12N3/00
Inventor 李玲陈凯杨凯刘宝军扈进冬李纪顺
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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