Two-photon multi-focus microscopic imaging system and method based on spatial light modulator

A spatial light modulator and microscopic imaging technology, which is applied in fluorescence/phosphorescence, instruments, scientific instruments, etc., can solve problems such as poor flexibility and complex structure, and achieve the goals of improving flexibility, reducing complexity, and solving mechanical inertia problems Effect

Active Publication Date: 2021-03-26
SHENZHEN UNIV
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Problems solved by technology

[0005] In view of the deficiencies in the above-mentioned prior art, the purpose of the present invention is to provide a two-photon multi-focus microscopic imaging system and method based on a spatial l

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  • Two-photon multi-focus microscopic imaging system and method based on spatial light modulator
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  • Two-photon multi-focus microscopic imaging system and method based on spatial light modulator

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[0049]In order to make the object, technical solution and advantages of the present invention clearer and clearer, the present invention will be further described in detail below with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0050] In the embodiments and the scope of the patent application, unless there is a special limitation on the article, "a" and "the" can generally refer to a single one or a plurality of them.

[0051] In addition, if there are descriptions involving "first", "second" and so on in the embodiments of the present invention, the descriptions of "first", "second" and so on are only for descriptive purposes, and should not be interpreted as indicating or implying Its relative importance or implicitly indicates the number of technical features indicated. Thus, the features defined as "first" and ...

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Abstract

The invention provides a two-photon multi-focus microscopic imaging system and method based on a spatial light modulator. The two-photon multi-focus microscopic imaging system comprises a laser lightsource, a spatial light modulator, an objective lens, a detector and a control terminal; the spatial light modulator is used for carrying out phase modulation on a laser beam according to the importedsuperimposed phase diagram which is switched at equal intervals to generate a multi-focus array; the superimposed phase diagram is formed by superposing a multi-focus array phase diagram and a linearphase grating phase diagram; the objective lens is used for projecting the multi-focus array to a sample surface to generate a multi-focus fluorescence signal; the detector is used for acquiring a multi-focus fluorescence signal to obtain a plurality of images; and the control terminal is used for processing the plurality of images to obtain a super-resolution image. According to the invention, the multi-focus array phase diagram and the linear phase grating phase diagram are loaded on the spatial light modulator at the same time, so the generation of the multi-focus array and the high-precision parallel digital random addressing scanning and excitation imaging of the sample surface are carried out, and a problem of mechanical inertia of a two-photon multi-focus microimaging system is solved; the complexity of the system is reduced, and the flexibility is improved.

Description

technical field [0001] The invention belongs to the technical field of optical imaging, and in particular relates to a two-photon multi-focus microscopic imaging system and method based on a spatial light modulator. Background technique [0002] Optical microscopic imaging system is one of the important tools for biologists to study biological phenomena and life activities. The traditional optical microscopic imaging system uses wide-field illumination, which limits its imaging depth and imaging resolution. Although laser confocal microscopy can achieve super-resolution imaging, its resolution is affected by the size of the pinhole. Although the image scanning microscope can improve the signal-to-noise ratio of the image, the imaging speed is slow. The multi-focus scanning microscope adopts a parallel scanning method, which can increase the imaging speed while improving the imaging depth. [0003] Existing multi-focus scanning microscopes are divided into single-photon mul...

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Application Information

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IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/6458G01N21/01
Inventor 于斌喻欢欢张晨爽屈军乐林丹樱
Owner SHENZHEN UNIV
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