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Lipase with improved heat resistance

A lipase and sequence technology, applied in the field of lipase, can solve the problems of limited application of lipase, low thermal stability, increased industrial production cost, etc.

Inactive Publication Date: 2021-03-30
东莞泛亚太生物科技有限公司
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Problems solved by technology

Although many kinds of lipases from Rhizopus have been available for use in industrial production, most of the natural Rhizopus lipases are mesophilic lipases with low thermal stability, which greatly increases the cost of industrial production and limits the production capacity of fats. Application of enzymes in industrial production

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  • Lipase with improved heat resistance
  • Lipase with improved heat resistance
  • Lipase with improved heat resistance

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Embodiment Construction

[0016] Some typical embodiments embodying the features and advantages of the present application will be described in detail in the description in the following paragraphs. It should be understood that the application is capable of various changes in different embodiments without departing from the scope of the application, and that the description and drawings therein are illustrative in nature and not limiting. this application.

[0017] The lipase of the present application is lipase (Rhizopus chinensis lipase, RCL) secreted by the filamentous fungus Rhizopus chinensis (Rhizopus chinensis). The optimal activity of this enzyme is at 40°C and pH 7.5. The present application separates the lipase gene RCL from Rhizopus sinensis and constructs it into a vector, and introduces it into Pichia pastoris, which is commonly used in industry, to express its protein. In order to improve the heat resistance of lipase RCL, this application uses protein structure analysis (PDB: 4L3W) to f...

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Abstract

The invention relates to lipase capable of improving heat resistance. The amino acid sequence of the lipase is a sequence obtained by replacing and modifying glutamic acid at the 292th position of SEQID No.2 with phenylalanine or isoleucine.

Description

technical field [0001] The present application relates to a lipase, especially a lipase with improved heat resistance. Background technique [0002] Lipase (EC 3.1.1.3) is a serine hydrolase that can hydrolyze triglycerides into fatty acids, diglycerides, monoglycerides, and glycerol; it can also catalyze acidolysis, alcoholysis, and aminolysis of esters And reactions such as transesterification and ester synthesis. Lipase has been widely used in food processing, oil processing, leather processing, bioenergy and feed addition because of its unique catalytic activity. Although many kinds of lipases from Rhizopus have been available for use in industrial production, most of the natural Rhizopus lipases are mesophilic lipases with low thermal stability, which greatly increases the cost of industrial production and limits the production capacity of fats. Application of enzymes in industrial production. [0003] Therefore, the present application intends to improve the heat re...

Claims

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Application Information

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IPC IPC(8): C12N9/20C12N15/55
CPCC12N9/20C12Y301/01003
Inventor 黄婷沅郑雅珊吴姿慧林正言赖惠琳郑成彬
Owner 东莞泛亚太生物科技有限公司
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