Preparation method and application of green alga polysaccharide derivative

A technology of chlorophyll polysaccharides and derivatives, which is applied in the field of preparation of chlorophyll polysaccharide derivatives, and can solve problems such as the lack of chlorophyll polysaccharide derivatives, lack of functional activity of Enteromorpha polysaccharides, and restrictions on the application of chlorophyll polysaccharides and their derivatives , to achieve the effect of expanding the level of resource utilization, improving the level of resource utilization, and enhancing the antioxidant capacity

Pending Publication Date: 2021-04-06
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few reports on the application of chlorella polysaccharide derivatives in fertilizers, and there is also a lack of research on the effects of the preparation methods of Enteromorpha polysaccharides on the functional activities of enteromorpha polysaccharide derivatives, which restricts the application of chlorella polysaccharides and their derivatives in fertilizers and feedstuffs. Applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing a chlorella polysaccharide derivative, specifically comprising the following steps: dissolving 100 mg of enzymatically hydrolyzed enteromorpha polysaccharide in 10 mL of 20% sodium hydroxide solution, adding 20 mL of isopropanol while stirring, ice bathing, and stirring for 3 h Finally, make a uniform suspension, and gradually add 25mL of isopropanol-chloroacetic acid mixture dropwise, gradually raise the temperature to 60°C, stir for 3h, stop the reaction, and cool to room temperature; adjust the pH value to neutral with 1mol / L hydrochloric acid , the reaction solution was placed in a dialysis bag with a molecular weight cut-off of 3500 for dialysis for 48 hours; the rotary evaporation solution was freeze-dried to obtain carboxymethylated Enteromorpha polysaccharide; wherein, the preparation method of the isopropanol-chloroacetic acid mixture is: Dissolve 30g of chloroacetic acid in 250mL of isopropanol, then add 100mL of NaOH solution with a mass...

Embodiment 2

[0034] A method for preparing a chlorella polysaccharide derivative, specifically comprising the following steps: dissolving 100 mg of enzymatically hydrolyzed enteromorpha polysaccharide in 10 mL of 20% sodium hydroxide solution, adding 20 mL of isopropanol while stirring, ice bathing, and stirring for 3 h Finally, make a uniform suspension, and gradually add 25mL of isopropanol-chloroacetic acid mixture dropwise, gradually raise the temperature to 60°C, stir for 3h, stop the reaction, and cool to room temperature; adjust the pH value to neutral with 1mol / L hydrochloric acid , the reaction solution was placed in a dialysis bag with a molecular weight cut-off of 3500 for dialysis for 48 hours; the rotary evaporation solution was freeze-dried to obtain carboxymethylated Enteromorpha polysaccharide; wherein, the preparation method of the isopropanol-chloroacetic acid mixture is: Dissolve 30g of chloroacetic acid in 250mL of isopropanol, then add 100mL of NaOH solution with a mass...

Embodiment 3

[0041] A method for preparing a chlorella polysaccharide derivative, specifically comprising the following steps: dissolving 100 mg of enzymatically hydrolyzed enteromorpha polysaccharide in 10 mL of 20% sodium hydroxide solution, adding 20 mL of isopropanol while stirring, ice bathing, and stirring for 3 h Finally, make a uniform suspension, and gradually add 25mL of isopropanol-chloroacetic acid mixture dropwise, gradually raise the temperature to 60°C, stir for 3h, stop the reaction, and cool to room temperature; adjust the pH value to neutral with 1mol / L hydrochloric acid , the reaction solution was placed in a dialysis bag with a molecular weight cut-off of 3500 for dialysis for 48 hours; the rotary evaporation solution was freeze-dried to obtain carboxymethylated Enteromorpha polysaccharide; wherein, the preparation method of the isopropanol-chloroacetic acid mixture is: Dissolve 30g of chloroacetic acid in 250mL of isopropanol, then add 100mL of NaOH solution with a mass...

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PUM

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Abstract

The invention discloses a preparation method and application of a green alga polysaccharide derivative, and belongs to the technical field of marine organisms, and the preparation method comprises the following steps: degrading enteromorpha prolifera and other green alga by using a biological enzymolysis method to extract green alga polysaccharide, then carrying out carboxymethyl modification on green alga polysaccharide branched chains, and introducing active functional groups, so the solubility, electronegativity and antibacterial property of the carboxymethylated polysaccharide are enhanced, and the antioxidant and anti-tumor activity of the carboxymethylated polysaccharide is improved, the immunomodulatory activity of the carboxymethylated polysaccharide is enhanced, the application of the carboxymethylated polysaccharide in fertilizers and feeds is realized, and the resource utilization level of green alga polysaccharides is improved. Experimental results show that the antioxidant capacity of the green alga polysaccharide derivative is remarkably improved, the product functionality can be remarkably improved when the green alga polysaccharide derivative is applied to the fields of feed, fertilizer and the like, the resource utilization level of green alga resources is expanded, and the green alga polysaccharide derivative has good market application prospects.

Description

technical field [0001] The invention relates to a preparation method and application of a chlorophyll polysaccharide derivative, in particular to a method for preparing a chlorophyll polysaccharide derivative through a biological enzymatic hydrolysis method and chemical modification technology, and belongs to the technical field of marine biology. Background technique [0002] my country is rich in seaweed resources, and its resource utilization has become the focus of my country's marine economic development. Enteromorpha is a large-scale economical green algae, commonly known as moss strips and seaweed, belonging to the phylum Chlorophyta, Ulvaceae of the order Ulva. Research has found that Enteromorpha is rich in carbohydrates, protein, crude fiber, amino acids, fatty acids, polysaccharides, vitamins and various mineral elements, among which Enteromorpha polysaccharides have the highest content, mainly composed of rhamnose, xylose, glucose and a small amount of mannose I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C05F11/00A23K20/163
CPCC08B37/006C08B37/0003C05F11/00A23K20/163
Inventor 庄松娟王海华单俊伟
Owner 青岛海大生物集团股份有限公司
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