Purpose of lactobacillus rhamnosus Probio-M9 and product thereof for preparing medicine used for treating autism spectrum disorders
A technology of Lactobacillus rhamnosus and autism, applied in the direction of bacteria, Lactobacillus, and drug combination used in food preparation, can solve the problem of incomplete clear etiology and pathogenesis of ASD, and achieve broad application prospects and promotion. Value, anxiety-relieving behavior, relatively low-cost effect
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Embodiment 1
[0063] Example 1 Social Behavior Test—Three Box Social Test
[0064] experiment method:
[0065] (1) Adaptation stage: put the tested mice into the device (40cm×60cm×22cm), and allow them to move freely in 3 connected chambers for 10 minutes to adapt to the environment.
[0066] (2) Testing phase:
[0067] Take out the well-adapted test mice, and randomly put them into an empty cage (denoted as "empty cage") and a cage with strange mice (denoted as "mouse 1") in the left and right chambers respectively.
[0068] Gently put the tested mice that have been adapted before into the middle chamber to make them move for 10 minutes, and record the number of times the tested mice stay in the left and right chambers respectively (n 空 and n 陌1 ) and the time to interact with the empty cage and strange mice respectively (T 空 and T 陌1 ), and gently remove the tested mouse after the end.
[0069] Put another strange mouse (denoted as "mouse 2") into the empty cage, and then put the ...
Embodiment 2
[0076] Example 2 Stereotyped Behavior Test—Grooming Test
[0077] experiment method:
[0078] (1) Adaptation stage: Take the mice from the animal room to the test room 1 hour before the start of the video recording behavior, and do 1 hour of environmental adaptation, and the room is illuminated by about 40 Lux. Each test time is from 17:00 to 21:00. During the experiment, the mice were gently moved into the transparent observation box (specification L×W×H: 20cm×20cm×50cm) and the data were collected at the same time.
[0079] (2) Test phase: each mouse was put into a clean observation box with bedding on the bottom, and the video camera was placed directly above the observation box. Under low light conditions (about 40Lux), after each pre-adaptation to the observation box for 10 minutes, the behavior of the mice was tested and recorded for 10 minutes. During the whole process, the experimenters kept quiet and kept away from the test mice. The observers kept the same standa...
Embodiment example 3
[0087] Implementation Case 3 Cognitive Behavioral Test—New Object Recognition
[0088] experiment method:
[0089] (1) Adaptation period: On the first day, the tested mice were adapted to the test room for 1 hour, and then put into the open field box (without objects in the box) to adapt for 20 minutes.
[0090] (2) Familiarization period: on the second day (after 24 hours of adaptation), the tested mice were adapted to the test room for 1 hour, then put the tested mice into the open field box to adapt for at least 5 minutes, and then put the tested mice out of the open field box. Take it out and place it in a clean temporary cage for about 2 minutes. Then place two yellow cylindrical bottles of the same size, color and texture at the left and right ends of the open field box as familiar objects, and then put the tested mice into the box with their backs facing the familiar objects, and record within 5 minutes When the tested mice explored familiar objects, the timing stan...
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