Method for enhancing aluminum resistance of peas

A pea, aluminum-resistant technology, applied in the field of planting, can solve the problems of limiting crop growth, reducing crop yield, impact, etc., to achieve the effects of alleviating aluminum toxicity, reducing aluminum accumulation, and reducing the rate of programmed cell death

Active Publication Date: 2021-04-20
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aluminum toxicity severely limits crop growth, reduces crop yields, and has a huge impact on world food

Method used

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  • Method for enhancing aluminum resistance of peas
  • Method for enhancing aluminum resistance of peas
  • Method for enhancing aluminum resistance of peas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Seed soaking and accelerating germination: Soak peas in 7.5% NaClO for 30 minutes to disinfect and accelerate germination, then wash 5 times with sterile ultrapure water, once every 5 minutes, and wash them in 0.5mmol / L CaCl after removing impurities. 2 Seeds soaked in the solution for 10 hours, kept at 24°C and dark room conditions;

[0020] 2) Aeroponics: Put the fully imbibed pea seeds into the aeroponic box, and add 0.5mmol / LCaCl to the aeroponic box 2 The solution was submerged in the aeroponic device, and the atomized culture was carried out for 48 hours. When CaCl 2 When the solution is not enough, it needs to be filled up, CaCl 2 The solution is replaced every 24 hours;

[0021] 3) Hydroponics: After pea seeds germinate, select seedlings with uniform root length and height of 4±0.1cm, prepare 1 / 4 concentration of Hoagland nutrient solution as culture solution A, and immerse the roots of the seedlings in the culture solution; and culture solution A After so...

Embodiment 2

[0024] 1) Seed soaking and accelerating germination: Soak peas in 7.5% NaClO for 30 minutes to disinfect and accelerate germination, then wash 5 times with sterile ultrapure water, once every 5 minutes, and wash them in 0.5mmol / L CaCl after removing impurities. 2 Seeds soaked in the solution for 10 hours, kept at 24°C and dark room conditions;

[0025] 2) Aeroponics: Put the fully imbibed pea seeds into the aeroponic box, and add 0.5mmol / LCaCl to the aeroponic box 2 The solution was submerged in the aeroponic device, and the atomized culture was carried out for 48 hours. When CaCl 2 When the solution is not enough, it needs to be filled up, CaCl 2 The solution is replaced every 24 hours;

[0026] 3) Hydroponics: After pea seeds germinate, select seedlings with uniform root length and height of 4±0.1cm, prepare 1 / 4 concentration of Hoagland nutrient solution as culture solution A, and immerse the roots of the seedlings in the culture solution; and culture solution A After so...

Embodiment 3

[0029] 1) Seed soaking and accelerating germination: Soak peas in 7.5% NaClO for 30 minutes to disinfect and accelerate germination, then wash 5 times with sterile ultrapure water, once every 5 minutes, and wash them in 0.5mmol / L CaCl after removing impurities. 2 Seeds soaked in the solution for 10 hours, kept at 24°C and dark room conditions;

[0030] 2) Aeroponics: Put the fully imbibed pea seeds into the aeroponic box, and add 0.5mmol / LCaCl to the aeroponic box 2 The solution was submerged in the aeroponic device, and the atomized culture was carried out for 48 hours. When CaCl 2 When the solution is not enough, it needs to be filled up, CaCl 2 The solution is replaced every 24 hours;

[0031] 3) Hydroponics: After pea seeds germinate, select seedlings with uniform root length and height of 4±0.1cm, prepare 1 / 4 concentration of Hoagland nutrient solution as culture solution A, and immerse the roots of the seedlings in the culture solution; and culture solution A After so...

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Abstract

The invention discloses a method for enhancing aluminum tolerance of peas. A GR24 solution with the concentration of 30-150 nmol/L is applied to pea plants. Compared with the prior art, GR24 is added in the step of low-acid adaptive treatment, so that the elongation of root systems can be remarkably promoted, the aluminum accumulation in root regions is effectively reduced, and particularly the aluminum content in root tip transition region is effectively reduced. The root tip transition region callose accumulation caused by aluminum toxicity is reduced, the ROS content of root tips is effectively reduced, and the programmed cell death speed caused by ROS is reduced to promote the growth of the root systems, so that the effects of relieving the aluminum toxicity and improving the aluminum tolerance of the plants are achieved.

Description

technical field [0001] The invention relates to the field of planting technology, in particular to a method for enhancing the aluminum tolerance of pea. Background technique [0002] Aluminum is the most abundant metal element in the earth's crust, accounting for 7.45% of the total weight of the earth's crust, and 50% of the acidic arable land is poisoned by aluminum. Aluminum toxicity is the main inhibitory factor for the growth of plants in acidic soils. Aluminum can affect the growth of crop roots at the nanomolar concentration level. Curved and irregularly hooked, the root cap falls off, and the biological oxidation capacity of the root system is significantly reduced. Aluminum poisoning severely limits crop growth, reduces crop yield, and has a huge impact on world food. Contents of the invention [0003] The invention discloses a method for enhancing the aluminum resistance of peas, so as to solve one or more technical problems in the prior art, and at least provid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00C05G3/00C05G5/20
CPCY02P60/21
Inventor 李学文李莉梁坚喻敏
Owner FOSHAN UNIVERSITY
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