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Filtering and extracting device for stem cell exosome

A technology for filtering and extracting exosomes, applied in the medical field, can solve the problems of affecting filtering and extracting effect, poor dilution effect, unevenness, etc., and achieve the effects of stable vibration, avoidance of load, and uniform and effective mixing.

Inactive Publication Date: 2021-04-20
菲尔生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When performing filtration and extraction of exosomes, the sample must be uniformly and effectively diluted to facilitate subsequent filtration processing. The existing filtration and extraction devices have poor dilution effects and often have unevenness, which affects the subsequent filtration and extraction effect; Therefore, it is necessary to improve the existing technology

Method used

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  • Filtering and extracting device for stem cell exosome
  • Filtering and extracting device for stem cell exosome
  • Filtering and extracting device for stem cell exosome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] see Figure 1-3 , a device for filtering and extracting stem cell exosomes, comprising a base 1, on which a dilution chamber 2 and a separation chamber 15 communicating with each other are arranged at intervals, the dilution chamber 2 and the base 1 are movably installed, and the upper end surface of the dilution chamber 2 A feed hopper 3 is arranged on one side, and a stirring mechanism is arranged in the dilution chamber 2; the stirring mechanism includes a vertical stirring shaft 8, and a plurality of stirring blades 9 are evenly arranged on the stirring shaft 8, and one end of the stirring shaft 8 extends to the diluting The top of the chamber 2 is connected with a drive mechanism; the separation chamber 15 is fixedly installed with the base 1, the inner side of the separation chamber 15 is provided with a filter layer 22, the inside of the separation chamber 15 is provided with a pumping mechanism, and one side of the separation chamber 15 is provided with a There ...

Embodiment 2

[0028] In order to facilitate the movement and placement of the device and reduce manpower handling, this embodiment is improved on the basis of Embodiment 1. The improvements are: the lower end of the base 1 is evenly equipped with several universal wheels, and the universal wheels A locking switch is arranged on the top to facilitate the movement and placement of the filtering and extracting device, saving manpower.

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Abstract

The invention discloses a filtering and extracting device for stem cell exosome. The filtering and extracting device comprises a base, wherein a diluting cavity and a separating cavity which communicating with each other are distributed on the base at intervals, the diluting cavity and the base are movably installed, a feeding hopper is distributed on one side of the upper end face of the diluting cavity, and a stirring mechanism is distributed in the diluting cavity. The filtering and extracting device for the stem cell exosome drives a driving wheel to operate through a driving motor, so that a stirring shaft drives stirring blades to perform orderly reciprocating rotation, and the cooperation of a vibration mechanism is assisted, so that a diluent and a sample are uniformly and effectively mixed, the dilution quality is ensured, subsequent filtering and extracting are facilitated, through cooperation of an eccentric wheel and a reset spring, a rubber plug can slide in an orderly reciprocating mode along the inner wall of a separation cavity, the filtering and extracting speed can be conveniently controlled, the load exceeding a filtering layer is avoided, and a vertical guide spring is installed on an assembly rod, so that the dilution cavity is oscillated stably, and splashing of the sample and the diluent is avoided.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a device for filtering and extracting stem cell exosomes. Background technique [0002] Stem cells can be said to be a hot spot that has to be paid attention to in the field of biomedicine in recent years. It has great potential in the improvement of human diseases, the delay of aging, and the regeneration of tissues; in many reports on the progress of stem cell research , there is a figure that often appears, that is, exosomes. Exosomes refer to small membrane vesicles (30-150nm) that contain complex RNA and proteins. Nowadays, they specifically refer to disc-shaped vesicles with a diameter of 40-100nm , exosomes are secreted by cells, and a variety of cells can secrete exosomes under normal and pathological conditions. Stem cell exosomes are vesicles secreted by stem cells with a diameter of 40-100 nm. When performing filtration and extraction of exosomes, the sample must be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/12C12M1/02
Inventor 朱学义刘岩
Owner 菲尔生物工程技术有限公司
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