Dehalogenase HldD1, encoding gene thereof and application of dehalogenase HldD1

A dehalogenase and encoding technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of toxicity, environmental and health consequences, and seriousness of microorganisms
CN112680427AActive Publication Date: 2021-04-20INST OF MICROBIOLOGY - CHINESE ACAD OF SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Publication Date
2021-04-20

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Abstract

The invention discloses dehalogenase HldD1, an encoding gene thereof and application of the dehalogenase HldD1. The invention provides a protein represented by a sequence 2 of a sequence list. Experiments prove that the dehalogenase HldD1 obtained through subjecting a gene sequence (sequence 1) disclosed by the invention to prokaryotic expression through Escherichia coli has high activity and has relatively high organic solvent stability separately under the treatment conditions of not higher than 20% of ethanol, not higher than 20% of acetonitrile and not higher than 40% of DMSO.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and specifically relates to a dehalogenase HldD1 and its coding gene and application. Background technique

[0002] Halogenated organic compounds are widely used in many fields. Among them, due to their extensive use in industrial enterprises and daily necessities, they have become an important class of environmental pollutants. These halogenated organics have continued to cause widespread environmental problems in the biosphere for many years due to their very stable chemical bonds. Halogenated organics are the most used and studied class of organics, mainly due to their relatively simple synthetic routes. However, due to the generally high stability of halogenated organic substances and their toxicity to microorganisms that may degrade them, the biodegradability of halogenated organic substances has been poor for many years. Excessive exposure to these halogenated pollutants can lead to serious ...

Claims

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