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Preparation method of hepatic failure rat model

A rat model, technology of liver failure, applied in the field of preparation of rat models of liver failure, can solve the problem of no therapeutic effect on acute liver failure, and achieve the effect of reducing liver cell apoptosis

Pending Publication Date: 2021-04-23
海南省人民医院
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AI Technical Summary

Problems solved by technology

BMSC transplantation via intraperitoneal injection has no therapeutic effect on acute liver failure in rats due to defective stem cell homing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market.

[0014] 1.1 General materials:

[0015] 1) Experimental animals: 50 healthy male SD rats, weighing about 300 to 400 grams after 79 days of breeding, SPF clean grade (purchased from Changsha Tianqin Biological Co., Ltd.), and provided with sufficient water and food in separate cages.

[0016] 2) Main reagents: fetal bovine serum, double antibody, 0.25% tr...

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Abstract

The invention particularly relates to a preparation method of a hepatic failure rat model. The preparation method comprises the following steps that (1) rats are anesthetized with 2mL of chloral hydrate through intraperitoneal injection, 5ml / kg of 50% carbon tetrachloride solution is pushed and injected through the intraperitoneal by using an injector once every Monday and Friday, continuous intraperitoneal injection is conducted for 3 weeks, and then blood is sampled through orbits to test the hepatic function; (2) the rats are randomly divided into five groups, stem cells are transplanted through portal veins, and then the successfully induced stem cells are injected at the cell concentration of 1*10<7> cells / ml through portal veins; (3) indexes are observed, specifically, I, survival conditions are observed after rat hepatic failure is induced by carbon tetrachloride, and II, hepatic function measuring is conducted, specifically, the indexes such as glutamic-pyruvic transaminase, glutamic oxalacetic transaminase, albumin, pathological sections and the like are measured; and (4) statistical analysis is conducted, specifically, analyzing is conducted by applying SPSS 17.0 software, and data is uniformly expressed in x+ / -s. The method is simple, and the animal model preparation success rate is high.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of a rat model of liver failure. Background technique [0002] The advantages of human bone marrow mesenchymal stem cells (BMSCs) are that they are easier to culture and purify in vitro, have strong proliferation ability in vitro, and still have strong multidirectional potential differentiation after 20-30 generations of culture ability; at the same time, it has significant advantages such as low immunogenicity and strong adhesion. In this study, we used carbon tetrachloride intraperitoneal injection to induce the establishment of an acute liver failure model. Human bone marrow mesenchymal stem cells were used as donors, and transplanted into rats through the portal vein to observe changes in liver tissue and biochemical indicators. Feasibility of clinical application of stem cells in the treatment of liver failure. [0003] Studies have sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/105A01K2267/03
Inventor 武金才李灼日严阳刚李兴陈家诚唐荣许达峰陈良陈骋
Owner 海南省人民医院
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