Method for detecting content of kanamycin in milk based on up-conversion and BHQ3 specific system

A kanamycin and detection method technology, applied in the detection field of kanamycin content, can solve the problems of cumbersome pretreatment and detection steps, high detection cost, long time, etc., achieve wide linear detection range, eliminate interference, Effect of Strong Fluorescent Responsivity
CN112697761AActive Publication Date: 2021-04-23JIANGSU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGSU UNIV
Publication Date
2021-04-23

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Abstract

The invention relates to a method for detecting the content of kanamycin in milk based on an up-conversion and BHQ3 specific system, and belongs to the technical field of food safety detection. The method comprises the steps: using an ethylene glycol mixed solution of yttrium chloride hexahydrate, ytterbium chloride hexahydrate, erbium chloride hexahydrate, polyethyleneimine, sodium chloride and ammonium fluoride as a raw material, and perparing an up-conversion fluorescent nano material; connecting the up-conversion material with a kanamycin aptamer through a glutaraldehyde cross-linking method; mixing and incubating with a kanamycin aptamer complementary chain BHQ3 solution to obtain a specific detection system; after the kanamycin solution is added, the fluorescence intensity signal characteristic value of the detection solution is determined as a vertical coordinate, the kanamycin concentration is taken as a horizontal coordinate, a kanamycin detection standard curve is established, and the kanamycin content in the milk to be detected is determined; by constructing a steady-state specific kanamycin fluorescence detection system, high-sensitivity and specific detection of kanamycin in milk is realized, and s a wide linear detection range and a low detection limit can be achieved.
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Description

technical field

[0001] The invention belongs to the technical field of food safety detection and specifically designs a detection method for kanamycin content in food based on up-conversion fluorescence and a BHQ3 quencher. Background technique

[0002] Kanamycin is an aminoglycoside antibiotic that binds to 30S ribosomes to cause misreading of mRNA codes, thereby inhibiting protein synthesis. Kanamycin has a great inhibitory effect on the growth and survival of certain microorganisms, and can be used to treat infections caused by Gram-positive and Gram-negative bacteria, and is widely used in food production and animal husbandry. At the same time, the unreasonable use of kanamycin will lead to residues in meat, milk and other animal foods, and then enter the human body through the food chain, which can cause great toxicity to the human body, including nephrotoxicity, antibiotic resistance , neuromuscular blockade and allergic reactions. In order to protect human health, t...

Claims

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