Method for detecting content of kanamycin in milk based on up-conversion and BHQ3 specific system
A kanamycin and detection method technology, applied in the detection field of kanamycin content, can solve the problems of cumbersome pretreatment and detection steps, high detection cost, long time, etc., achieve wide linear detection range, eliminate interference, Effect of Strong Fluorescent Responsivity
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Embodiment 1
[0026] Step 1, preparation of up-conversion fluorescent nanomaterials: Accurately weigh 0.53mmol yttrium chloride hexahydrate, 0.2mmol ytterbium chloride hexahydrate, 0.25mmol gadolinium chloride hexahydrate, 0.02mmol erbium chloride hexahydrate, 2.5mmol chloride Dissolve sodium and 0.3g polyethyleneimine in 15ml of ethylene glycol and stir vigorously until the solution is transparent. 5mmol of ammonium fluoride was dissolved in 5mL of ethylene glycol and added dropwise to the above transparent solution and stirred for 30min; the mixed solution was transferred to a 50mL reaction kettle and placed in an oven at 200°C for 12h; naturally cooled to room temperature, washed with ethanol and The water mixed solution washes the reaction product 2-3 times, and vacuum-dries to obtain the up-conversion fluorescent nanometer material.
[0027] Step 2, preparation of fluorescent nanoprobes: dissolving the up-conversion fluorescent nanomaterial obtained by the one-step synthesis method in ...
Embodiment 2
[0035] Embodiment 2: to 7 kanamycin standard samples, adopt method and step described in the embodiment of the present invention 1, measure kanamycin content, as shown in measurement result table 1, can find out that method of the present invention and The high-performance liquid chromatography has a high degree of coincidence, indicating that the method of the present invention has a high degree of accuracy.
[0036] Table 1 The result (unit, μM) of kanamycin in the method of the present invention and high performance liquid chromatography detection standard sample
[0037]
[0038] t=0.340.05
[0039] For 3 milk samples, adopt the method and steps described in the embodiment of the present invention 1, measure the content of kanamycin in the milk, as shown in Table 2 of the measurement results, it can be seen that the method of the present invention has a better effect in actual samples. The accuracy is good, and the application prospect is good.
[0040] Table 1 The in...
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