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Method for detecting content of kanamycin in milk based on up-conversion and BHQ3 specific system

A kanamycin and detection method technology, applied in the detection field of kanamycin content, can solve the problems of cumbersome pretreatment and detection steps, high detection cost, long time, etc., achieve wide linear detection range, eliminate interference, Effect of Strong Fluorescent Responsivity

Active Publication Date: 2021-04-23
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies in the existing detection technology, such as: high detection cost, long time, cumbersome preprocessing and detection steps, etc., the present invention provides An up-conversion fluorescence detection method for kanamycin in food was developed. Fluorescent donors were prepared by nano-controllable self-assembly, and a steady-state specific kanamycin detection system was constructed to eliminate the influence of background fluorescence and interference from other factors. Achieving low-cost, highly sensitive and specific detection of kanamycin in food

Method used

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  • Method for detecting content of kanamycin in milk based on up-conversion and BHQ3 specific system
  • Method for detecting content of kanamycin in milk based on up-conversion and BHQ3 specific system
  • Method for detecting content of kanamycin in milk based on up-conversion and BHQ3 specific system

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Embodiment 1

[0026] Step 1, preparation of up-conversion fluorescent nanomaterials: Accurately weigh 0.53mmol yttrium chloride hexahydrate, 0.2mmol ytterbium chloride hexahydrate, 0.25mmol gadolinium chloride hexahydrate, 0.02mmol erbium chloride hexahydrate, 2.5mmol chloride Dissolve sodium and 0.3g polyethyleneimine in 15ml of ethylene glycol and stir vigorously until the solution is transparent. 5mmol of ammonium fluoride was dissolved in 5mL of ethylene glycol and added dropwise to the above transparent solution and stirred for 30min; the mixed solution was transferred to a 50mL reaction kettle and placed in an oven at 200°C for 12h; naturally cooled to room temperature, washed with ethanol and The water mixed solution washes the reaction product 2-3 times, and vacuum-dries to obtain the up-conversion fluorescent nanometer material.

[0027] Step 2, preparation of fluorescent nanoprobes: dissolving the up-conversion fluorescent nanomaterial obtained by the one-step synthesis method in ...

Embodiment 2

[0035] Embodiment 2: to 7 kanamycin standard samples, adopt method and step described in the embodiment of the present invention 1, measure kanamycin content, as shown in measurement result table 1, can find out that method of the present invention and The high-performance liquid chromatography has a high degree of coincidence, indicating that the method of the present invention has a high degree of accuracy.

[0036] Table 1 The result (unit, μM) of kanamycin in the method of the present invention and high performance liquid chromatography detection standard sample

[0037]

[0038] t=0.340.05

[0039] For 3 milk samples, adopt the method and steps described in the embodiment of the present invention 1, measure the content of kanamycin in the milk, as shown in Table 2 of the measurement results, it can be seen that the method of the present invention has a better effect in actual samples. The accuracy is good, and the application prospect is good.

[0040] Table 1 The in...

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Abstract

The invention relates to a method for detecting the content of kanamycin in milk based on an up-conversion and BHQ3 specific system, and belongs to the technical field of food safety detection. The method comprises the steps: using an ethylene glycol mixed solution of yttrium chloride hexahydrate, ytterbium chloride hexahydrate, erbium chloride hexahydrate, polyethyleneimine, sodium chloride and ammonium fluoride as a raw material, and perparing an up-conversion fluorescent nano material; connecting the up-conversion material with a kanamycin aptamer through a glutaraldehyde cross-linking method; mixing and incubating with a kanamycin aptamer complementary chain BHQ3 solution to obtain a specific detection system; after the kanamycin solution is added, the fluorescence intensity signal characteristic value of the detection solution is determined as a vertical coordinate, the kanamycin concentration is taken as a horizontal coordinate, a kanamycin detection standard curve is established, and the kanamycin content in the milk to be detected is determined; by constructing a steady-state specific kanamycin fluorescence detection system, high-sensitivity and specific detection of kanamycin in milk is realized, and s a wide linear detection range and a low detection limit can be achieved.

Description

technical field [0001] The invention belongs to the technical field of food safety detection and specifically designs a detection method for kanamycin content in food based on up-conversion fluorescence and a BHQ3 quencher. Background technique [0002] Kanamycin is an aminoglycoside antibiotic that binds to 30S ribosomes to cause misreading of mRNA codes, thereby inhibiting protein synthesis. Kanamycin has a great inhibitory effect on the growth and survival of certain microorganisms, and can be used to treat infections caused by Gram-positive and Gram-negative bacteria, and is widely used in food production and animal husbandry. At the same time, the unreasonable use of kanamycin will lead to residues in meat, milk and other animal foods, and then enter the human body through the food chain, which can cause great toxicity to the human body, including nephrotoxicity, antibiotic resistance , neuromuscular blockade and allergic reactions. In order to protect human health, t...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 张运莲陈全胜刘蕊欧阳琴李欢欢
Owner JIANGSU UNIV
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