An aptamer sensor based on upconversion and black phosphorus nanosheets for the detection of aflatoxin b1

A technique for the detection of aflatoxin and aflatoxin B1, which is applied in the field of detection of aflatoxin B1, and can solve problems such as the inability to meet the requirements of rapid and high-sensitivity detection of AFB1 content, the low detection accuracy and sensitivity of AFB1, and the inability to achieve specific detection of AFB1. Achieve the effects of eliminating background fluorescence and interference from other molecules, good practical prospects, and ensuring food security

Active Publication Date: 2022-07-01
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, traditional aflatoxin detection methods, such as high performance liquid chromatography, enzyme-linked immunosorbent assay, etc., are not only expensive in equipment, high in detection costs, and cumbersome in steps, but more importantly, they cannot achieve specific detection of AFB1. Therefore, the requirements for rapid and highly sensitive detection of AFB1 content cannot be met, and the detection accuracy and sensitivity of AFB1 are at a low level

Method used

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  • An aptamer sensor based on upconversion and black phosphorus nanosheets for the detection of aflatoxin b1
  • An aptamer sensor based on upconversion and black phosphorus nanosheets for the detection of aflatoxin b1
  • An aptamer sensor based on upconversion and black phosphorus nanosheets for the detection of aflatoxin b1

Examples

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Embodiment 1

[0046] Embodiment 1 Detection of aflatoxin B1 content in peanut oil

[0047] A method for detecting aflatoxin B1 based on an aptamer sensor based on upconversion and black phosphorus nanosheets, comprising the following steps:

[0048] Step 1, preparation of amino functionalized up-conversion nanomaterials: accurately weigh 0.2366g yttrium chloride hexahydrate, 0.0775g ytterbium chloride hexahydrate, 0.0076g erbium chloride hexahydrate, 0.1461g sodium chloride and 0.1852g fluoride Ammonium was added to 15 mL of ethylene glycol solution, dissolved by ultrasonic for 10 min, transferred to a 100 mL round-bottomed flask with magnetic stirring for 30 min, and mixed evenly; 0.2 g of polyethyleneimine was added to the mixed solution under light-proof conditions and fully stirred for 10 min; The uniformly mixed solution was transferred to a polytetrafluoroethylene-lined autoclave, and reacted at 200 °C for 12 h to obtain a reaction product; after cooling, the reaction product was prec...

Embodiment 2

[0062] Example 2 Detection of aflatoxin B1 content in wheat

[0063] A method for detecting aflatoxin B1 based on an aptamer sensor based on upconversion and black phosphorus nanosheets, comprising the following steps:

[0064] Step 1, preparation of amino functionalized up-conversion nanomaterials: accurately weigh 0.2366g yttrium chloride hexahydrate, 0.0775g ytterbium chloride hexahydrate, 0.0076g erbium chloride hexahydrate, 0.1461g sodium chloride and 0.1852g fluoride Ammonium was added to 15 mL of ethylene glycol solution, dissolved by ultrasonic for 15 min, transferred to a 100 mL round-bottomed flask with magnetic stirring for 40 min, and mixed evenly; under light-proof conditions, 0.2 g of polyethyleneimine was added to the mixed solution and fully stirred for 10 min; The uniformly mixed solution was transferred to a polytetrafluoroethylene-lined autoclave, and reacted at 180 °C for 8 hours to obtain the reaction product; after cooling, the reaction product was precip...

Embodiment 3

[0072] Example 3 Detection of aflatoxin B1 content in peanuts

[0073] A method for detecting aflatoxin B1 based on an aptamer sensor based on upconversion and black phosphorus nanosheets, comprising the following steps:

[0074] Step 1, preparation of amino functionalized upconversion nanomaterials: Accurately weigh 0.2366g yttrium chloride hexahydrate, 0.0775g ytterbium chloride hexahydrate, 0.0076g erbium chloride hexahydrate, 0.1461g sodium chloride and 0.1852g Ammonium fluoride was added to 15 mL of ethylene glycol solution, dissolved by ultrasonic for 15 min, transferred to a 100 mL round-bottomed flask with magnetic stirring for 40 min, and mixed evenly; under light-proof conditions, 0.2 g of polyethyleneimine was added to the mixed solution and fully stirred for 10 min ; Transfer the well-mixed solution to a polytetrafluoroethylene-lined high-pressure reaction kettle, and react at 190 ° C for 12 h to obtain the reaction product; after the reaction product is cooled, pr...

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Abstract

The invention discloses an aflatoxin B1 detection method based on an aptamer sensor of up-conversion and black phosphorus nanosheets, and relates to the field of food safety detection. The steps are as follows: step 1: prepare amino functionalized up-conversion nanomaterials; step 2: connect aflatoxin B1 aptamer on the surface of amino functionalized up-conversion nanomaterials; step 3: prepare black phosphorus nanosheets; step 4: combine step 2. The obtained upconversion nanoparticles with surface-linked aptamers are dissolved in the buffer, add the dispersion liquid made of black phosphorus nanosheets obtained in step 3, and incubate and bind to obtain a specific detection system; step 5: establish aflatoxin B1 Content detection standard curve; Step 6: Detect the content of aflatoxin B1 in the sample. By constructing a fluorescence detection system of aflatoxin B1, the invention realizes high specificity and sensitivity detection of aflatoxin B1 in food, has a wider concentration detection range and lower detection limit, and has good practical prospects.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a detection method for aflatoxin B1 based on an aptamer sensor of upconversion and black phosphorus nanosheets. Background technique [0002] Aflatoxins are secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus, and are highly toxic and carcinogenic mycotoxins. Aflatoxins mainly include B group (B1 and B2), G group (G1 and G2), and M group (M1 and M2), among which aflatoxin B1 (AFB1) is the most toxic and is listed by the International Agency for Research on Substances in Cancer. Class I carcinogens. [0003] AFB1 can lead to a series of health hazards such as severe liver damage, liver cancer, and growth disorders in children. Unfortunately, according to the Food and Agriculture Organization of the United Nations, about 25% of crops worldwide are affected by mycotoxins, and AFB1 contamination alone accounts for a quarter of all crops af...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 吴继忠陈全胜欧阳琴荣雅文李欢欢王丽徐夏霏
Owner JIANGSU UNIV
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